Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20320 and PS417_20325 are separated by 118 nucleotides PS417_20325 and PS417_20330 overlap by 32 nucleotides PS417_20330 and PS417_20335 are separated by 296 nucleotides
PS417_20320: PS417_20320 - exonuclease, at 4,412,310 to 4,412,930
_20320
PS417_20325: PS417_20325 - hypothetical protein, at 4,413,049 to 4,414,131
_20325
PS417_20330: PS417_20330 - S-adenosylhomocysteine hydrolase, at 4,414,100 to 4,414,519
_20330
PS417_20335: PS417_20335 - hypothetical protein, at 4,414,816 to 4,415,634
_20335
Position (kb)
4413
4414
4415 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4412.056 kb on + strand at 4412.083 kb on + strand at 4412.083 kb on + strand at 4412.084 kb on - strand at 4412.084 kb on - strand at 4412.087 kb on + strand at 4412.087 kb on + strand at 4412.088 kb on - strand at 4412.088 kb on - strand at 4412.314 kb on + strand at 4412.314 kb on + strand at 4412.314 kb on + strand at 4412.377 kb on + strand, within PS417_20320 at 4412.377 kb on + strand, within PS417_20320 at 4413.002 kb on - strand at 4413.011 kb on + strand at 4413.012 kb on - strand at 4413.063 kb on + strand at 4413.064 kb on - strand at 4413.195 kb on + strand, within PS417_20325 at 4413.380 kb on + strand, within PS417_20325 at 4413.381 kb on - strand, within PS417_20325 at 4413.549 kb on + strand, within PS417_20325 at 4413.550 kb on - strand, within PS417_20325 at 4413.550 kb on - strand, within PS417_20325 at 4413.551 kb on + strand, within PS417_20325 at 4413.551 kb on + strand, within PS417_20325 at 4413.551 kb on + strand, within PS417_20325 at 4413.552 kb on - strand, within PS417_20325 at 4413.552 kb on - strand, within PS417_20325 at 4413.627 kb on + strand, within PS417_20325 at 4413.627 kb on + strand at 4413.628 kb on - strand, within PS417_20325 at 4413.657 kb on - strand, within PS417_20325 at 4413.657 kb on - strand, within PS417_20325 at 4413.674 kb on + strand, within PS417_20325 at 4413.675 kb on - strand, within PS417_20325 at 4413.754 kb on + strand, within PS417_20325 at 4413.794 kb on - strand, within PS417_20325 at 4413.794 kb on - strand at 4413.813 kb on - strand, within PS417_20325 at 4413.873 kb on + strand, within PS417_20325 at 4414.101 kb on + strand at 4414.335 kb on + strand, within PS417_20330 at 4414.519 kb on + strand at 4414.547 kb on + strand at 4414.563 kb on + strand at 4414.564 kb on - strand at 4414.564 kb on - strand at 4414.719 kb on + strand at 4414.720 kb on - strand at 4414.807 kb on + strand at 4414.808 kb on - strand at 4414.825 kb on + strand at 4414.825 kb on + strand at 4414.826 kb on - strand at 4414.826 kb on - strand at 4414.826 kb on - strand at 4414.834 kb on + strand at 4414.834 kb on + strand at 4414.834 kb on + strand at 4414.834 kb on + strand at 4414.835 kb on - strand at 4414.892 kb on - strand at 4414.892 kb on - strand at 4414.963 kb on + strand at 4414.963 kb on + strand, within PS417_20335 at 4414.964 kb on - strand, within PS417_20335 at 4415.029 kb on + strand, within PS417_20335 at 4415.029 kb on + strand, within PS417_20335 at 4415.030 kb on - strand, within PS417_20335 at 4415.030 kb on - strand, within PS417_20335 at 4415.113 kb on + strand, within PS417_20335 at 4415.113 kb on + strand, within PS417_20335 at 4415.119 kb on + strand, within PS417_20335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,412,056 + +0.0 4,412,083 + -2.1 4,412,083 + +1.6 4,412,084 - -1.0 4,412,084 - +0.7 4,412,087 + -0.2 4,412,087 + +0.1 4,412,088 - +1.2 4,412,088 - -2.5 4,412,314 + -0.7 4,412,314 + +0.2 4,412,314 + +1.0 4,412,377 + PS417_20320 0.11 -1.2 4,412,377 + PS417_20320 0.11 -2.1 4,413,002 - -0.6 4,413,011 + -0.2 4,413,012 - -1.3 4,413,063 + -2.5 4,413,064 - -2.5 4,413,195 + PS417_20325 0.13 -2.7 4,413,380 + PS417_20325 0.31 +4.5 4,413,381 - PS417_20325 0.31 +0.4 4,413,549 + PS417_20325 0.46 -0.8 4,413,550 - PS417_20325 0.46 -1.5 4,413,550 - PS417_20325 0.46 +0.7 4,413,551 + PS417_20325 0.46 +0.7 4,413,551 + PS417_20325 0.46 -0.2 4,413,551 + PS417_20325 0.46 +0.2 4,413,552 - PS417_20325 0.46 -0.3 4,413,552 - PS417_20325 0.46 -0.1 4,413,627 + PS417_20325 0.53 +0.1 4,413,627 + +0.4 4,413,628 - PS417_20325 0.53 +1.2 4,413,657 - PS417_20325 0.56 +1.9 4,413,657 - PS417_20325 0.56 +0.8 4,413,674 + PS417_20325 0.58 +2.1 4,413,675 - PS417_20325 0.58 -0.8 4,413,754 + PS417_20325 0.65 +4.1 4,413,794 - PS417_20325 0.69 -0.2 4,413,794 - +0.0 4,413,813 - PS417_20325 0.71 +0.4 4,413,873 + PS417_20325 0.76 -1.9 4,414,101 + +2.5 4,414,335 + PS417_20330 0.56 -0.3 4,414,519 + -0.7 4,414,547 + +1.3 4,414,563 + +0.3 4,414,564 - -2.2 4,414,564 - -1.7 4,414,719 + +0.3 4,414,720 - +0.4 4,414,807 + +0.1 4,414,808 - +0.1 4,414,825 + -0.4 4,414,825 + +0.9 4,414,826 - +0.3 4,414,826 - -0.7 4,414,826 - +0.8 4,414,834 + -0.7 4,414,834 + -1.2 4,414,834 + -1.7 4,414,834 + -0.9 4,414,835 - -0.8 4,414,892 - +2.1 4,414,892 - -0.4 4,414,963 + -1.7 4,414,963 + PS417_20335 0.18 +1.2 4,414,964 - PS417_20335 0.18 +0.3 4,415,029 + PS417_20335 0.26 +0.0 4,415,029 + PS417_20335 0.26 -1.2 4,415,030 - PS417_20335 0.26 -0.3 4,415,030 - PS417_20335 0.26 -0.8 4,415,113 + PS417_20335 0.36 +1.0 4,415,113 + PS417_20335 0.36 -0.6 4,415,119 + PS417_20335 0.37 -1.7
Or see this region's nucleotide sequence