Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19080 and PS417_19085 are separated by 454 nucleotides PS417_19085 and PS417_19090 overlap by 14 nucleotides PS417_19090 and PS417_19095 overlap by 1 nucleotides PS417_19095 and PS417_19100 are separated by 65 nucleotides PS417_19100 and PS417_19105 are separated by 15 nucleotides PS417_19105 and PS417_19110 are separated by 82 nucleotides
PS417_19080: PS417_19080 - tRNA-Asn, at 4,127,945 to 4,128,017
_19080
PS417_19085: PS417_19085 - hypothetical protein, at 4,128,472 to 4,128,705
_19085
PS417_19090: PS417_19090 - hypothetical protein, at 4,128,692 to 4,128,880
_19090
PS417_19095: PS417_19095 - hypothetical protein, at 4,128,880 to 4,129,164
_19095
PS417_19100: PS417_19100 - hypothetical protein, at 4,129,230 to 4,129,460
_19100
PS417_19105: PS417_19105 - hypothetical protein, at 4,129,476 to 4,129,697
_19105
PS417_19110: PS417_19110 - hypothetical protein, at 4,129,780 to 4,130,079
_19110
Position (kb)
4128
4129
4130 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4127.941 kb on + strand at 4128.056 kb on + strand at 4128.168 kb on - strand at 4128.258 kb on + strand at 4128.368 kb on + strand at 4128.368 kb on + strand at 4128.509 kb on + strand at 4128.514 kb on - strand, within PS417_19085 at 4128.516 kb on - strand, within PS417_19085 at 4128.568 kb on + strand, within PS417_19085 at 4128.569 kb on - strand, within PS417_19085 at 4128.617 kb on - strand, within PS417_19085 at 4128.650 kb on - strand, within PS417_19085 at 4128.694 kb on - strand at 4128.698 kb on - strand at 4128.737 kb on - strand, within PS417_19090 at 4128.756 kb on + strand, within PS417_19090 at 4128.757 kb on - strand, within PS417_19090 at 4128.795 kb on - strand, within PS417_19090 at 4128.920 kb on + strand, within PS417_19095 at 4128.926 kb on - strand, within PS417_19095 at 4129.036 kb on - strand at 4129.040 kb on - strand, within PS417_19095 at 4129.151 kb on - strand at 4129.507 kb on + strand, within PS417_19105 at 4129.508 kb on - strand, within PS417_19105 at 4129.528 kb on + strand, within PS417_19105 at 4129.528 kb on + strand, within PS417_19105 at 4129.529 kb on - strand, within PS417_19105 at 4129.529 kb on - strand, within PS417_19105 at 4129.529 kb on - strand, within PS417_19105 at 4129.529 kb on - strand, within PS417_19105 at 4129.532 kb on + strand, within PS417_19105 at 4129.532 kb on + strand, within PS417_19105 at 4129.532 kb on + strand, within PS417_19105 at 4129.532 kb on + strand, within PS417_19105 at 4129.532 kb on + strand, within PS417_19105 at 4129.532 kb on + strand, within PS417_19105 at 4129.533 kb on - strand, within PS417_19105 at 4129.533 kb on - strand, within PS417_19105 at 4129.533 kb on - strand, within PS417_19105 at 4129.533 kb on - strand, within PS417_19105 at 4129.533 kb on - strand, within PS417_19105 at 4129.534 kb on + strand at 4129.535 kb on - strand, within PS417_19105 at 4129.548 kb on + strand, within PS417_19105 at 4129.578 kb on - strand, within PS417_19105 at 4129.644 kb on + strand at 4129.645 kb on - strand, within PS417_19105 at 4129.646 kb on + strand, within PS417_19105 at 4129.672 kb on + strand at 4129.672 kb on + strand, within PS417_19105 at 4129.672 kb on + strand, within PS417_19105 at 4129.673 kb on - strand, within PS417_19105 at 4129.673 kb on - strand, within PS417_19105 at 4129.695 kb on + strand at 4129.695 kb on + strand at 4129.832 kb on - strand, within PS417_19110 at 4129.832 kb on - strand, within PS417_19110 at 4129.848 kb on + strand, within PS417_19110 at 4129.848 kb on + strand, within PS417_19110 at 4129.849 kb on - strand, within PS417_19110 at 4129.852 kb on + strand, within PS417_19110 at 4129.852 kb on + strand, within PS417_19110 at 4129.925 kb on + strand, within PS417_19110 at 4129.926 kb on - strand, within PS417_19110 at 4129.926 kb on - strand, within PS417_19110 at 4129.959 kb on + strand, within PS417_19110 at 4129.959 kb on + strand, within PS417_19110 at 4130.001 kb on + strand, within PS417_19110 at 4130.001 kb on + strand, within PS417_19110 at 4130.014 kb on + strand, within PS417_19110 at 4130.157 kb on + strand at 4130.158 kb on - strand at 4130.158 kb on - strand at 4130.158 kb on - strand at 4130.158 kb on - strand at 4130.161 kb on + strand at 4130.161 kb on + strand at 4130.162 kb on - strand at 4130.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,127,941 + -0.0 4,128,056 + -0.5 4,128,168 - +0.4 4,128,258 + -0.8 4,128,368 + -1.7 4,128,368 + +0.0 4,128,509 + -0.3 4,128,514 - PS417_19085 0.18 +1.8 4,128,516 - PS417_19085 0.19 +0.2 4,128,568 + PS417_19085 0.41 -0.7 4,128,569 - PS417_19085 0.41 -0.7 4,128,617 - PS417_19085 0.62 -0.6 4,128,650 - PS417_19085 0.76 -0.1 4,128,694 - -0.1 4,128,698 - +0.4 4,128,737 - PS417_19090 0.24 -0.2 4,128,756 + PS417_19090 0.34 -1.3 4,128,757 - PS417_19090 0.34 +0.1 4,128,795 - PS417_19090 0.54 -0.1 4,128,920 + PS417_19095 0.14 -2.0 4,128,926 - PS417_19095 0.16 -2.0 4,129,036 - -0.9 4,129,040 - PS417_19095 0.56 -0.7 4,129,151 - -0.5 4,129,507 + PS417_19105 0.14 -0.6 4,129,508 - PS417_19105 0.14 +0.2 4,129,528 + PS417_19105 0.23 -0.1 4,129,528 + PS417_19105 0.23 -0.4 4,129,529 - PS417_19105 0.24 -0.7 4,129,529 - PS417_19105 0.24 -0.0 4,129,529 - PS417_19105 0.24 -0.3 4,129,529 - PS417_19105 0.24 -1.1 4,129,532 + PS417_19105 0.25 +0.4 4,129,532 + PS417_19105 0.25 -0.2 4,129,532 + PS417_19105 0.25 +0.8 4,129,532 + PS417_19105 0.25 +1.9 4,129,532 + PS417_19105 0.25 -0.2 4,129,532 + PS417_19105 0.25 +0.4 4,129,533 - PS417_19105 0.26 -0.2 4,129,533 - PS417_19105 0.26 -0.5 4,129,533 - PS417_19105 0.26 +0.2 4,129,533 - PS417_19105 0.26 +1.1 4,129,533 - PS417_19105 0.26 +1.8 4,129,534 + +1.7 4,129,535 - PS417_19105 0.27 -0.2 4,129,548 + PS417_19105 0.32 -1.3 4,129,578 - PS417_19105 0.46 +0.8 4,129,644 + +0.9 4,129,645 - PS417_19105 0.76 +0.2 4,129,646 + PS417_19105 0.77 -0.4 4,129,672 + -0.9 4,129,672 + PS417_19105 0.88 -0.1 4,129,672 + PS417_19105 0.88 -1.4 4,129,673 - PS417_19105 0.89 -0.0 4,129,673 - PS417_19105 0.89 +1.4 4,129,695 + -0.5 4,129,695 + -1.0 4,129,832 - PS417_19110 0.17 +0.9 4,129,832 - PS417_19110 0.17 -1.6 4,129,848 + PS417_19110 0.23 +3.1 4,129,848 + PS417_19110 0.23 +1.1 4,129,849 - PS417_19110 0.23 +0.7 4,129,852 + PS417_19110 0.24 -0.5 4,129,852 + PS417_19110 0.24 -0.4 4,129,925 + PS417_19110 0.48 -1.3 4,129,926 - PS417_19110 0.49 +0.7 4,129,926 - PS417_19110 0.49 +0.5 4,129,959 + PS417_19110 0.60 -0.4 4,129,959 + PS417_19110 0.60 +0.7 4,130,001 + PS417_19110 0.74 -0.3 4,130,001 + PS417_19110 0.74 +0.4 4,130,014 + PS417_19110 0.78 +1.2 4,130,157 + -0.2 4,130,158 - +1.7 4,130,158 - -0.5 4,130,158 - -0.1 4,130,158 - +0.7 4,130,161 + +0.1 4,130,161 + +0.7 4,130,162 - -1.9 4,130,162 - +0.9
Or see this region's nucleotide sequence