Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19075 and PS417_19080 are separated by 298 nucleotides PS417_19080 and PS417_19085 are separated by 454 nucleotides PS417_19085 and PS417_19090 overlap by 14 nucleotides PS417_19090 and PS417_19095 overlap by 1 nucleotides
PS417_19075: PS417_19075 - ABC transporter ATP-binding protein, at 4,126,315 to 4,127,646
_19075
PS417_19080: PS417_19080 - tRNA-Asn, at 4,127,945 to 4,128,017
_19080
PS417_19085: PS417_19085 - hypothetical protein, at 4,128,472 to 4,128,705
_19085
PS417_19090: PS417_19090 - hypothetical protein, at 4,128,692 to 4,128,880
_19090
PS417_19095: PS417_19095 - hypothetical protein, at 4,128,880 to 4,129,164
_19095
Position (kb)
4127
4128
4129 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4127.017 kb on + strand, within PS417_19075 at 4127.073 kb on + strand, within PS417_19075 at 4127.074 kb on - strand, within PS417_19075 at 4127.080 kb on + strand, within PS417_19075 at 4127.097 kb on + strand, within PS417_19075 at 4127.097 kb on + strand, within PS417_19075 at 4127.098 kb on - strand, within PS417_19075 at 4127.207 kb on + strand at 4127.207 kb on + strand, within PS417_19075 at 4127.207 kb on + strand, within PS417_19075 at 4127.318 kb on + strand, within PS417_19075 at 4127.319 kb on - strand, within PS417_19075 at 4127.454 kb on + strand, within PS417_19075 at 4127.500 kb on - strand, within PS417_19075 at 4127.539 kb on - strand at 4127.541 kb on - strand at 4127.650 kb on + strand at 4127.651 kb on - strand at 4127.796 kb on + strand at 4127.796 kb on + strand at 4127.941 kb on + strand at 4128.056 kb on + strand at 4128.168 kb on - strand at 4128.258 kb on + strand at 4128.368 kb on + strand at 4128.368 kb on + strand at 4128.509 kb on + strand at 4128.514 kb on - strand, within PS417_19085 at 4128.516 kb on - strand, within PS417_19085 at 4128.568 kb on + strand, within PS417_19085 at 4128.569 kb on - strand, within PS417_19085 at 4128.617 kb on - strand, within PS417_19085 at 4128.650 kb on - strand, within PS417_19085 at 4128.694 kb on - strand at 4128.698 kb on - strand at 4128.737 kb on - strand, within PS417_19090 at 4128.756 kb on + strand, within PS417_19090 at 4128.757 kb on - strand, within PS417_19090 at 4128.795 kb on - strand, within PS417_19090 at 4128.920 kb on + strand, within PS417_19095 at 4128.926 kb on - strand, within PS417_19095
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,127,017 + PS417_19075 0.53 +0.4 4,127,073 + PS417_19075 0.57 +0.2 4,127,074 - PS417_19075 0.57 +1.7 4,127,080 + PS417_19075 0.57 -0.1 4,127,097 + PS417_19075 0.59 -0.1 4,127,097 + PS417_19075 0.59 -0.5 4,127,098 - PS417_19075 0.59 +1.1 4,127,207 + -2.0 4,127,207 + PS417_19075 0.67 -0.2 4,127,207 + PS417_19075 0.67 +0.2 4,127,318 + PS417_19075 0.75 +1.2 4,127,319 - PS417_19075 0.75 +1.1 4,127,454 + PS417_19075 0.86 +0.2 4,127,500 - PS417_19075 0.89 +1.3 4,127,539 - +1.5 4,127,541 - +1.2 4,127,650 + -1.3 4,127,651 - +0.7 4,127,796 + -0.4 4,127,796 + -0.1 4,127,941 + -0.0 4,128,056 + -0.5 4,128,168 - +0.4 4,128,258 + -0.8 4,128,368 + -1.7 4,128,368 + +0.0 4,128,509 + -0.3 4,128,514 - PS417_19085 0.18 +1.8 4,128,516 - PS417_19085 0.19 +0.2 4,128,568 + PS417_19085 0.41 -0.7 4,128,569 - PS417_19085 0.41 -0.7 4,128,617 - PS417_19085 0.62 -0.6 4,128,650 - PS417_19085 0.76 -0.1 4,128,694 - -0.1 4,128,698 - +0.4 4,128,737 - PS417_19090 0.24 -0.2 4,128,756 + PS417_19090 0.34 -1.3 4,128,757 - PS417_19090 0.34 +0.1 4,128,795 - PS417_19090 0.54 -0.1 4,128,920 + PS417_19095 0.14 -2.0 4,128,926 - PS417_19095 0.16 -2.0
Or see this region's nucleotide sequence