Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19065 and PS417_19070 overlap by 12 nucleotides PS417_19070 and PS417_19075 are separated by 4 nucleotides PS417_19075 and PS417_19080 are separated by 298 nucleotides PS417_19080 and PS417_19085 are separated by 454 nucleotides
PS417_19065: PS417_19065 - monooxygenase, at 4,125,255 to 4,125,593
_19065
PS417_19070: PS417_19070 - hypothetical protein, at 4,125,582 to 4,126,310
_19070
PS417_19075: PS417_19075 - ABC transporter ATP-binding protein, at 4,126,315 to 4,127,646
_19075
PS417_19080: PS417_19080 - tRNA-Asn, at 4,127,945 to 4,128,017
_19080
PS417_19085: PS417_19085 - hypothetical protein, at 4,128,472 to 4,128,705
_19085
Position (kb)
4126
4127
4128 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4125.522 kb on + strand, within PS417_19065 at 4125.660 kb on + strand, within PS417_19070 at 4125.674 kb on - strand, within PS417_19070 at 4125.688 kb on - strand, within PS417_19070 at 4125.945 kb on + strand, within PS417_19070 at 4125.945 kb on + strand, within PS417_19070 at 4125.946 kb on - strand, within PS417_19070 at 4125.947 kb on + strand at 4125.947 kb on + strand, within PS417_19070 at 4125.948 kb on - strand, within PS417_19070 at 4125.948 kb on - strand, within PS417_19070 at 4126.056 kb on + strand, within PS417_19070 at 4126.057 kb on - strand, within PS417_19070 at 4126.076 kb on - strand, within PS417_19070 at 4126.085 kb on + strand, within PS417_19070 at 4126.086 kb on - strand, within PS417_19070 at 4126.258 kb on + strand at 4126.259 kb on - strand at 4126.377 kb on + strand at 4126.378 kb on - strand at 4126.499 kb on - strand at 4126.509 kb on - strand, within PS417_19075 at 4126.509 kb on - strand, within PS417_19075 at 4126.627 kb on + strand, within PS417_19075 at 4126.628 kb on - strand, within PS417_19075 at 4126.628 kb on - strand, within PS417_19075 at 4126.665 kb on + strand, within PS417_19075 at 4126.666 kb on - strand, within PS417_19075 at 4126.666 kb on - strand, within PS417_19075 at 4126.666 kb on - strand, within PS417_19075 at 4126.666 kb on - strand, within PS417_19075 at 4126.666 kb on - strand, within PS417_19075 at 4126.667 kb on + strand, within PS417_19075 at 4126.667 kb on + strand, within PS417_19075 at 4126.668 kb on - strand, within PS417_19075 at 4126.673 kb on + strand, within PS417_19075 at 4126.673 kb on + strand, within PS417_19075 at 4126.674 kb on - strand, within PS417_19075 at 4126.674 kb on - strand, within PS417_19075 at 4126.674 kb on - strand at 4126.761 kb on - strand, within PS417_19075 at 4126.799 kb on + strand, within PS417_19075 at 4126.938 kb on - strand, within PS417_19075 at 4127.017 kb on + strand, within PS417_19075 at 4127.073 kb on + strand, within PS417_19075 at 4127.074 kb on - strand, within PS417_19075 at 4127.080 kb on + strand, within PS417_19075 at 4127.097 kb on + strand, within PS417_19075 at 4127.097 kb on + strand, within PS417_19075 at 4127.098 kb on - strand, within PS417_19075 at 4127.207 kb on + strand at 4127.207 kb on + strand, within PS417_19075 at 4127.207 kb on + strand, within PS417_19075 at 4127.318 kb on + strand, within PS417_19075 at 4127.319 kb on - strand, within PS417_19075 at 4127.454 kb on + strand, within PS417_19075 at 4127.500 kb on - strand, within PS417_19075 at 4127.539 kb on - strand at 4127.541 kb on - strand at 4127.650 kb on + strand at 4127.651 kb on - strand at 4127.796 kb on + strand at 4127.796 kb on + strand at 4127.941 kb on + strand at 4128.056 kb on + strand at 4128.168 kb on - strand at 4128.258 kb on + strand at 4128.368 kb on + strand at 4128.368 kb on + strand at 4128.509 kb on + strand at 4128.514 kb on - strand, within PS417_19085 at 4128.516 kb on - strand, within PS417_19085 at 4128.568 kb on + strand, within PS417_19085 at 4128.569 kb on - strand, within PS417_19085 at 4128.617 kb on - strand, within PS417_19085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,125,522 + PS417_19065 0.79 -0.0 4,125,660 + PS417_19070 0.11 -0.3 4,125,674 - PS417_19070 0.13 -0.7 4,125,688 - PS417_19070 0.15 +0.7 4,125,945 + PS417_19070 0.50 -0.8 4,125,945 + PS417_19070 0.50 -0.6 4,125,946 - PS417_19070 0.50 -1.0 4,125,947 + +0.7 4,125,947 + PS417_19070 0.50 +0.1 4,125,948 - PS417_19070 0.50 -1.7 4,125,948 - PS417_19070 0.50 -1.7 4,126,056 + PS417_19070 0.65 -1.0 4,126,057 - PS417_19070 0.65 +0.5 4,126,076 - PS417_19070 0.68 -0.6 4,126,085 + PS417_19070 0.69 -0.2 4,126,086 - PS417_19070 0.69 +1.7 4,126,258 + +0.2 4,126,259 - -0.8 4,126,377 + +0.9 4,126,378 - +0.9 4,126,499 - +2.3 4,126,509 - PS417_19075 0.15 -0.2 4,126,509 - PS417_19075 0.15 +1.4 4,126,627 + PS417_19075 0.23 -0.1 4,126,628 - PS417_19075 0.23 +1.5 4,126,628 - PS417_19075 0.23 +0.9 4,126,665 + PS417_19075 0.26 +0.5 4,126,666 - PS417_19075 0.26 -0.2 4,126,666 - PS417_19075 0.26 +0.6 4,126,666 - PS417_19075 0.26 +0.9 4,126,666 - PS417_19075 0.26 +1.1 4,126,666 - PS417_19075 0.26 +1.2 4,126,667 + PS417_19075 0.26 -0.7 4,126,667 + PS417_19075 0.26 +1.0 4,126,668 - PS417_19075 0.27 +1.0 4,126,673 + PS417_19075 0.27 +1.1 4,126,673 + PS417_19075 0.27 +0.3 4,126,674 - PS417_19075 0.27 +1.1 4,126,674 - PS417_19075 0.27 +1.4 4,126,674 - +1.4 4,126,761 - PS417_19075 0.33 -0.2 4,126,799 + PS417_19075 0.36 -0.3 4,126,938 - PS417_19075 0.47 +1.5 4,127,017 + PS417_19075 0.53 +0.4 4,127,073 + PS417_19075 0.57 +0.2 4,127,074 - PS417_19075 0.57 +1.7 4,127,080 + PS417_19075 0.57 -0.1 4,127,097 + PS417_19075 0.59 -0.1 4,127,097 + PS417_19075 0.59 -0.5 4,127,098 - PS417_19075 0.59 +1.1 4,127,207 + -2.0 4,127,207 + PS417_19075 0.67 -0.2 4,127,207 + PS417_19075 0.67 +0.2 4,127,318 + PS417_19075 0.75 +1.2 4,127,319 - PS417_19075 0.75 +1.1 4,127,454 + PS417_19075 0.86 +0.2 4,127,500 - PS417_19075 0.89 +1.3 4,127,539 - +1.5 4,127,541 - +1.2 4,127,650 + -1.3 4,127,651 - +0.7 4,127,796 + -0.4 4,127,796 + -0.1 4,127,941 + -0.0 4,128,056 + -0.5 4,128,168 - +0.4 4,128,258 + -0.8 4,128,368 + -1.7 4,128,368 + +0.0 4,128,509 + -0.3 4,128,514 - PS417_19085 0.18 +1.8 4,128,516 - PS417_19085 0.19 +0.2 4,128,568 + PS417_19085 0.41 -0.7 4,128,569 - PS417_19085 0.41 -0.7 4,128,617 - PS417_19085 0.62 -0.6
Or see this region's nucleotide sequence