Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18230 and PS417_18235 are separated by 73 nucleotides PS417_18235 and PS417_18240 are separated by 41 nucleotides PS417_18240 and PS417_18245 overlap by 4 nucleotides
PS417_18230: PS417_18230 - amidase, at 3,972,172 to 3,973,686
_18230
PS417_18235: PS417_18235 - nitrile hydratase, at 3,973,760 to 3,974,353
_18235
PS417_18240: PS417_18240 - nitrile hydratase, at 3,974,395 to 3,975,057
_18240
PS417_18245: PS417_18245 - hypothetical protein, at 3,975,054 to 3,976,313
_18245
Position (kb)
3974
3975
3976 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3973.489 kb on + strand, within PS417_18230 at 3973.490 kb on - strand, within PS417_18230 at 3973.618 kb on + strand at 3973.733 kb on - strand at 3973.932 kb on + strand, within PS417_18235 at 3974.016 kb on - strand, within PS417_18235 at 3974.241 kb on - strand, within PS417_18235 at 3974.457 kb on + strand at 3974.477 kb on + strand, within PS417_18240 at 3974.477 kb on + strand, within PS417_18240 at 3974.507 kb on - strand, within PS417_18240 at 3974.615 kb on - strand, within PS417_18240 at 3974.623 kb on + strand, within PS417_18240 at 3974.623 kb on + strand, within PS417_18240 at 3974.624 kb on - strand, within PS417_18240 at 3974.624 kb on - strand, within PS417_18240 at 3974.800 kb on + strand, within PS417_18240 at 3974.801 kb on - strand, within PS417_18240 at 3974.801 kb on - strand, within PS417_18240 at 3974.830 kb on + strand, within PS417_18240 at 3974.831 kb on - strand, within PS417_18240 at 3974.831 kb on - strand, within PS417_18240 at 3974.935 kb on + strand, within PS417_18240 at 3974.935 kb on + strand, within PS417_18240 at 3974.936 kb on - strand, within PS417_18240 at 3974.954 kb on + strand, within PS417_18240 at 3975.016 kb on + strand at 3975.183 kb on - strand, within PS417_18245 at 3975.562 kb on + strand, within PS417_18245 at 3975.591 kb on + strand, within PS417_18245 at 3975.592 kb on - strand, within PS417_18245 at 3975.831 kb on + strand, within PS417_18245 at 3975.831 kb on + strand, within PS417_18245 at 3975.831 kb on + strand, within PS417_18245 at 3975.832 kb on - strand, within PS417_18245 at 3976.014 kb on + strand, within PS417_18245 at 3976.014 kb on + strand, within PS417_18245 at 3976.014 kb on + strand, within PS417_18245 at 3976.014 kb on + strand, within PS417_18245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,973,489 + PS417_18230 0.87 -0.8 3,973,490 - PS417_18230 0.87 -1.5 3,973,618 + +0.4 3,973,733 - +0.9 3,973,932 + PS417_18235 0.29 +0.4 3,974,016 - PS417_18235 0.43 -1.2 3,974,241 - PS417_18235 0.81 -0.3 3,974,457 + -0.7 3,974,477 + PS417_18240 0.12 -1.2 3,974,477 + PS417_18240 0.12 +0.5 3,974,507 - PS417_18240 0.17 +0.5 3,974,615 - PS417_18240 0.33 -0.2 3,974,623 + PS417_18240 0.34 +0.6 3,974,623 + PS417_18240 0.34 -1.9 3,974,624 - PS417_18240 0.35 -0.9 3,974,624 - PS417_18240 0.35 +1.3 3,974,800 + PS417_18240 0.61 +0.3 3,974,801 - PS417_18240 0.61 +0.3 3,974,801 - PS417_18240 0.61 +0.1 3,974,830 + PS417_18240 0.66 +0.0 3,974,831 - PS417_18240 0.66 +0.3 3,974,831 - PS417_18240 0.66 -0.1 3,974,935 + PS417_18240 0.81 -0.3 3,974,935 + PS417_18240 0.81 +2.5 3,974,936 - PS417_18240 0.82 +1.1 3,974,954 + PS417_18240 0.84 +0.4 3,975,016 + -0.4 3,975,183 - PS417_18245 0.10 -0.5 3,975,562 + PS417_18245 0.40 +0.1 3,975,591 + PS417_18245 0.43 +0.4 3,975,592 - PS417_18245 0.43 +0.1 3,975,831 + PS417_18245 0.62 -2.5 3,975,831 + PS417_18245 0.62 +0.4 3,975,831 + PS417_18245 0.62 -0.4 3,975,832 - PS417_18245 0.62 +1.0 3,976,014 + PS417_18245 0.76 -1.2 3,976,014 + PS417_18245 0.76 -0.6 3,976,014 + PS417_18245 0.76 +1.0 3,976,014 + PS417_18245 0.76 +1.0
Or see this region's nucleotide sequence