Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01755 and PS417_01760 are separated by 15 nucleotides PS417_01760 and PS417_01765 are separated by 12 nucleotides PS417_01765 and PS417_01770 are separated by 129 nucleotides
PS417_01755: PS417_01755 - proline-specific permease, at 390,179 to 391,588
_01755
PS417_01760: PS417_01760 - imidazolonepropionase, at 391,604 to 392,809
_01760
PS417_01765: PS417_01765 - N-formylglutamate amidohydrolase, at 392,822 to 393,622
_01765
PS417_01770: PS417_01770 - glycine/betaine ABC transporter substrate-binding protein, at 393,752 to 394,687
_01770
Position (kb) 391
392
393 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 390.927 kb on - strand, within PS417_01755 at 390.927 kb on - strand, within PS417_01755 at 390.927 kb on - strand, within PS417_01755 at 390.955 kb on + strand, within PS417_01755 at 390.956 kb on - strand, within PS417_01755 at 390.960 kb on - strand, within PS417_01755 at 391.115 kb on + strand, within PS417_01755 at 391.116 kb on - strand, within PS417_01755 at 391.656 kb on - strand at 391.732 kb on + strand, within PS417_01760 at 391.733 kb on - strand at 391.733 kb on - strand, within PS417_01760 at 391.866 kb on - strand, within PS417_01760 at 391.998 kb on + strand at 392.098 kb on + strand, within PS417_01760 at 392.098 kb on + strand, within PS417_01760 at 392.153 kb on + strand, within PS417_01760 at 392.460 kb on - strand, within PS417_01760 at 392.747 kb on + strand at 392.817 kb on + strand at 392.817 kb on + strand at 392.817 kb on + strand at 392.818 kb on - strand at 392.818 kb on - strand at 392.818 kb on - strand at 392.818 kb on - strand at 393.687 kb on - strand at 393.721 kb on + strand at 393.722 kb on - strand at 393.726 kb on + strand at 393.726 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 390,927 - PS417_01755 0.53 +0.6 390,927 - PS417_01755 0.53 +0.6 390,927 - PS417_01755 0.53 +0.1 390,955 + PS417_01755 0.55 +0.2 390,956 - PS417_01755 0.55 -0.7 390,960 - PS417_01755 0.55 -0.8 391,115 + PS417_01755 0.66 -0.0 391,116 - PS417_01755 0.66 +0.3 391,656 - -0.7 391,732 + PS417_01760 0.11 -1.4 391,733 - +0.7 391,733 - PS417_01760 0.11 -0.7 391,866 - PS417_01760 0.22 +1.9 391,998 + -0.5 392,098 + PS417_01760 0.41 -1.1 392,098 + PS417_01760 0.41 -0.9 392,153 + PS417_01760 0.46 -0.9 392,460 - PS417_01760 0.71 -1.0 392,747 + +0.0 392,817 + -1.7 392,817 + -0.3 392,817 + -1.3 392,818 - -1.3 392,818 - +0.1 392,818 - -1.5 392,818 - -3.4 393,687 - -1.8 393,721 + +2.3 393,722 - -0.0 393,726 + -0.1 393,726 + -0.2
Or see this region's nucleotide sequence