Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17170 and PS417_17175 overlap by 4 nucleotides PS417_17175 and PS417_17185 are separated by 333 nucleotides
PS417_17170: PS417_17170 - carbon-nitrogen hydrolase, at 3,749,741 to 3,750,871
_17170
PS417_17175: PS417_17175 - AraC family transcriptional regulator, at 3,750,868 to 3,751,896
_17175
PS417_17185: PS417_17185 - pyruvate dehydrogenase, at 3,752,230 to 3,753,246
_17185
Position (kb)
3750
3751
3752 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3750.080 kb on + strand, within PS417_17170 at 3750.081 kb on - strand, within PS417_17170 at 3750.192 kb on - strand, within PS417_17170 at 3750.192 kb on - strand, within PS417_17170 at 3750.227 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.348 kb on - strand, within PS417_17170 at 3750.348 kb on - strand, within PS417_17170 at 3750.348 kb on - strand at 3750.350 kb on - strand, within PS417_17170 at 3750.419 kb on + strand, within PS417_17170 at 3750.420 kb on - strand, within PS417_17170 at 3750.436 kb on + strand, within PS417_17170 at 3750.498 kb on + strand, within PS417_17170 at 3750.498 kb on + strand, within PS417_17170 at 3750.499 kb on - strand, within PS417_17170 at 3750.700 kb on + strand, within PS417_17170 at 3750.701 kb on - strand, within PS417_17170 at 3750.859 kb on + strand at 3751.285 kb on + strand, within PS417_17175 at 3751.286 kb on - strand, within PS417_17175 at 3751.399 kb on + strand at 3751.526 kb on + strand, within PS417_17175 at 3751.527 kb on - strand, within PS417_17175 at 3751.527 kb on - strand, within PS417_17175 at 3751.753 kb on + strand, within PS417_17175 at 3751.753 kb on + strand, within PS417_17175 at 3751.792 kb on + strand, within PS417_17175 at 3751.792 kb on + strand, within PS417_17175 at 3751.792 kb on + strand, within PS417_17175 at 3751.907 kb on - strand at 3752.027 kb on + strand at 3752.028 kb on - strand at 3752.028 kb on - strand at 3752.107 kb on - strand at 3752.113 kb on - strand at 3752.124 kb on - strand at 3752.145 kb on - strand at 3752.199 kb on + strand at 3752.200 kb on - strand at 3752.200 kb on - strand at 3752.200 kb on - strand at 3752.311 kb on + strand at 3752.311 kb on + strand at 3752.425 kb on - strand, within PS417_17185 at 3752.447 kb on - strand, within PS417_17185 at 3752.605 kb on - strand, within PS417_17185 at 3752.611 kb on + strand, within PS417_17185 at 3752.612 kb on - strand, within PS417_17185 at 3752.671 kb on + strand, within PS417_17185 at 3752.695 kb on + strand, within PS417_17185 at 3752.695 kb on + strand, within PS417_17185 at 3752.695 kb on + strand, within PS417_17185 at 3752.695 kb on + strand, within PS417_17185 at 3752.698 kb on + strand, within PS417_17185 at 3752.773 kb on + strand at 3752.774 kb on - strand, within PS417_17185 at 3752.774 kb on - strand, within PS417_17185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,750,080 + PS417_17170 0.30 -0.8 3,750,081 - PS417_17170 0.30 +0.2 3,750,192 - PS417_17170 0.40 -0.1 3,750,192 - PS417_17170 0.40 +0.3 3,750,227 + PS417_17170 0.43 +0.0 3,750,347 + PS417_17170 0.54 +0.5 3,750,347 + PS417_17170 0.54 -2.5 3,750,347 + PS417_17170 0.54 -0.6 3,750,347 + PS417_17170 0.54 -0.0 3,750,347 + PS417_17170 0.54 -0.2 3,750,347 + PS417_17170 0.54 -0.2 3,750,348 - PS417_17170 0.54 -0.9 3,750,348 - PS417_17170 0.54 +0.1 3,750,348 - -0.7 3,750,350 - PS417_17170 0.54 +0.1 3,750,419 + PS417_17170 0.60 +0.2 3,750,420 - PS417_17170 0.60 +1.1 3,750,436 + PS417_17170 0.61 -0.3 3,750,498 + PS417_17170 0.67 -0.2 3,750,498 + PS417_17170 0.67 -0.6 3,750,499 - PS417_17170 0.67 -1.0 3,750,700 + PS417_17170 0.85 -0.4 3,750,701 - PS417_17170 0.85 -3.8 3,750,859 + -0.7 3,751,285 + PS417_17175 0.41 -0.4 3,751,286 - PS417_17175 0.41 +1.3 3,751,399 + -0.4 3,751,526 + PS417_17175 0.64 -0.0 3,751,527 - PS417_17175 0.64 -2.8 3,751,527 - PS417_17175 0.64 +1.6 3,751,753 + PS417_17175 0.86 +0.3 3,751,753 + PS417_17175 0.86 +0.4 3,751,792 + PS417_17175 0.90 +5.0 3,751,792 + PS417_17175 0.90 -1.8 3,751,792 + PS417_17175 0.90 +0.9 3,751,907 - -1.3 3,752,027 + -0.8 3,752,028 - +0.0 3,752,028 - +0.8 3,752,107 - +0.5 3,752,113 - +0.2 3,752,124 - -0.5 3,752,145 - -0.8 3,752,199 + +0.3 3,752,200 - +0.3 3,752,200 - +0.3 3,752,200 - +1.2 3,752,311 + +0.4 3,752,311 + +0.3 3,752,425 - PS417_17185 0.19 -0.2 3,752,447 - PS417_17185 0.21 -0.2 3,752,605 - PS417_17185 0.37 -0.0 3,752,611 + PS417_17185 0.37 -1.0 3,752,612 - PS417_17185 0.38 +0.2 3,752,671 + PS417_17185 0.43 -1.0 3,752,695 + PS417_17185 0.46 +0.7 3,752,695 + PS417_17185 0.46 -0.4 3,752,695 + PS417_17185 0.46 -0.4 3,752,695 + PS417_17185 0.46 +0.1 3,752,698 + PS417_17185 0.46 +0.1 3,752,773 + +0.2 3,752,774 - PS417_17185 0.53 +0.1 3,752,774 - PS417_17185 0.53 +0.0
Or see this region's nucleotide sequence