Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01675 and PS417_01680 are separated by 200 nucleotides PS417_01680 and PS417_01685 overlap by 1 nucleotides
PS417_01675: PS417_01675 - maltodextrin phosphorylase, at 368,200 to 370,650
_01675
PS417_01680: PS417_01680 - membrane protein, at 370,851 to 373,922
_01680
PS417_01685: PS417_01685 - membrane protein, at 373,922 to 375,277
_01685
Position (kb) 370
371
372
373
374 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 369.865 kb on - strand, within PS417_01675 at 369.875 kb on - strand, within PS417_01675 at 370.099 kb on + strand, within PS417_01675 at 370.284 kb on + strand, within PS417_01675 at 370.668 kb on - strand at 370.668 kb on - strand at 370.729 kb on + strand at 370.804 kb on + strand at 370.804 kb on + strand at 370.804 kb on + strand at 370.805 kb on - strand at 370.805 kb on - strand at 370.807 kb on + strand at 370.807 kb on + strand at 370.808 kb on - strand at 370.808 kb on - strand at 370.808 kb on - strand at 370.838 kb on - strand at 371.484 kb on - strand, within PS417_01680 at 371.526 kb on + strand at 371.527 kb on - strand, within PS417_01680 at 371.733 kb on + strand, within PS417_01680 at 371.733 kb on + strand, within PS417_01680 at 371.734 kb on - strand at 371.853 kb on + strand, within PS417_01680 at 371.910 kb on + strand, within PS417_01680 at 371.910 kb on + strand, within PS417_01680 at 371.910 kb on + strand, within PS417_01680 at 371.911 kb on - strand, within PS417_01680 at 371.943 kb on - strand, within PS417_01680 at 372.021 kb on + strand, within PS417_01680 at 372.021 kb on + strand, within PS417_01680 at 372.021 kb on + strand, within PS417_01680 at 372.021 kb on + strand, within PS417_01680 at 372.021 kb on + strand, within PS417_01680 at 372.040 kb on + strand, within PS417_01680 at 372.040 kb on + strand, within PS417_01680 at 372.041 kb on - strand, within PS417_01680 at 372.086 kb on - strand, within PS417_01680 at 372.138 kb on + strand, within PS417_01680 at 372.139 kb on - strand, within PS417_01680 at 372.139 kb on - strand, within PS417_01680 at 372.139 kb on - strand, within PS417_01680 at 372.195 kb on + strand, within PS417_01680 at 372.307 kb on + strand, within PS417_01680 at 372.308 kb on - strand, within PS417_01680 at 372.343 kb on + strand, within PS417_01680 at 372.343 kb on + strand, within PS417_01680 at 372.472 kb on + strand, within PS417_01680 at 372.472 kb on + strand, within PS417_01680 at 372.472 kb on + strand, within PS417_01680 at 372.473 kb on - strand, within PS417_01680 at 373.033 kb on - strand, within PS417_01680 at 373.033 kb on - strand, within PS417_01680 at 373.151 kb on - strand, within PS417_01680 at 373.269 kb on + strand, within PS417_01680 at 373.428 kb on + strand, within PS417_01680 at 373.429 kb on - strand, within PS417_01680 at 373.429 kb on - strand, within PS417_01680 at 373.486 kb on - strand, within PS417_01680 at 373.869 kb on + strand at 373.870 kb on - strand at 374.048 kb on - strand at 374.142 kb on - strand, within PS417_01685 at 374.142 kb on - strand, within PS417_01685 at 374.214 kb on - strand, within PS417_01685
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 369,865 - PS417_01675 0.68 +0.8 369,875 - PS417_01675 0.68 -0.6 370,099 + PS417_01675 0.77 -0.0 370,284 + PS417_01675 0.85 +0.2 370,668 - -2.5 370,668 - -0.1 370,729 + -0.1 370,804 + +0.2 370,804 + +1.5 370,804 + +0.9 370,805 - -2.3 370,805 - +0.1 370,807 + -0.0 370,807 + +0.5 370,808 - -0.6 370,808 - -0.1 370,808 - -0.6 370,838 - -1.8 371,484 - PS417_01680 0.21 -1.3 371,526 + +0.3 371,527 - PS417_01680 0.22 -2.3 371,733 + PS417_01680 0.29 -1.7 371,733 + PS417_01680 0.29 +0.2 371,734 - +0.9 371,853 + PS417_01680 0.33 -1.1 371,910 + PS417_01680 0.34 -1.7 371,910 + PS417_01680 0.34 -0.3 371,910 + PS417_01680 0.34 +0.3 371,911 - PS417_01680 0.35 -0.1 371,943 - PS417_01680 0.36 -0.2 372,021 + PS417_01680 0.38 -0.7 372,021 + PS417_01680 0.38 -1.0 372,021 + PS417_01680 0.38 +0.0 372,021 + PS417_01680 0.38 -1.2 372,021 + PS417_01680 0.38 +0.8 372,040 + PS417_01680 0.39 -1.0 372,040 + PS417_01680 0.39 -0.8 372,041 - PS417_01680 0.39 +0.0 372,086 - PS417_01680 0.40 +0.4 372,138 + PS417_01680 0.42 -1.4 372,139 - PS417_01680 0.42 +0.3 372,139 - PS417_01680 0.42 +0.8 372,139 - PS417_01680 0.42 +0.7 372,195 + PS417_01680 0.44 -1.3 372,307 + PS417_01680 0.47 -0.0 372,308 - PS417_01680 0.47 -1.1 372,343 + PS417_01680 0.49 -0.1 372,343 + PS417_01680 0.49 -0.6 372,472 + PS417_01680 0.53 -0.3 372,472 + PS417_01680 0.53 -1.9 372,472 + PS417_01680 0.53 -1.9 372,473 - PS417_01680 0.53 +0.1 373,033 - PS417_01680 0.71 -0.8 373,033 - PS417_01680 0.71 +0.1 373,151 - PS417_01680 0.75 +3.0 373,269 + PS417_01680 0.79 +0.6 373,428 + PS417_01680 0.84 -2.2 373,429 - PS417_01680 0.84 -0.1 373,429 - PS417_01680 0.84 +0.1 373,486 - PS417_01680 0.86 +0.2 373,869 + +1.4 373,870 - -0.0 374,048 - -0.3 374,142 - PS417_01685 0.16 -1.0 374,142 - PS417_01685 0.16 -0.3 374,214 - PS417_01685 0.22 -2.2
Or see this region's nucleotide sequence