Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15160 and PS417_15165 are separated by 306 nucleotides PS417_15165 and PS417_15170 are separated by 111 nucleotides
PS417_15160: PS417_15160 - hypothetical protein, at 3,321,246 to 3,322,274
_15160
PS417_15165: PS417_15165 - 1-carboxy-3-chloro-3,4-dihydroxycyclo hexa-1,5-diene dehydrogenase, at 3,322,581 to 3,323,696
_15165
PS417_15170: PS417_15170 - long-chain fatty acid--CoA ligase, at 3,323,808 to 3,325,511
_15170
Position (kb)
3322
3323
3324 Strain fitness (log2 ratio)
-1
0
1
2 at 3322.054 kb on + strand, within PS417_15160 at 3322.289 kb on + strand at 3322.290 kb on - strand at 3322.290 kb on - strand at 3322.306 kb on + strand at 3322.306 kb on + strand at 3322.351 kb on - strand at 3322.458 kb on - strand at 3322.459 kb on + strand at 3322.459 kb on + strand at 3322.459 kb on + strand at 3322.460 kb on - strand at 3322.460 kb on - strand at 3322.679 kb on - strand at 3322.805 kb on - strand, within PS417_15165 at 3322.968 kb on + strand at 3322.969 kb on - strand, within PS417_15165 at 3322.994 kb on + strand, within PS417_15165 at 3322.994 kb on + strand, within PS417_15165 at 3323.542 kb on + strand, within PS417_15165 at 3323.694 kb on + strand at 3323.695 kb on - strand at 3323.752 kb on + strand at 3323.879 kb on - strand at 3323.905 kb on + strand at 3323.905 kb on + strand at 3324.001 kb on - strand, within PS417_15170 at 3324.095 kb on + strand, within PS417_15170 at 3324.124 kb on + strand, within PS417_15170 at 3324.214 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,322,054 + PS417_15160 0.79 +0.1 3,322,289 + +0.1 3,322,290 - -1.2 3,322,290 - -1.0 3,322,306 + +0.4 3,322,306 + -1.4 3,322,351 - -0.0 3,322,458 - +0.0 3,322,459 + +0.2 3,322,459 + +0.5 3,322,459 + +0.4 3,322,460 - +0.5 3,322,460 - -0.0 3,322,679 - -0.2 3,322,805 - PS417_15165 0.20 +1.4 3,322,968 + +0.8 3,322,969 - PS417_15165 0.35 +0.9 3,322,994 + PS417_15165 0.37 -1.3 3,322,994 + PS417_15165 0.37 +0.6 3,323,542 + PS417_15165 0.86 +1.0 3,323,694 + -1.6 3,323,695 - +0.8 3,323,752 + -0.4 3,323,879 - -1.5 3,323,905 + -0.2 3,323,905 + -1.0 3,324,001 - PS417_15170 0.11 -0.4 3,324,095 + PS417_15170 0.17 -0.1 3,324,124 + PS417_15170 0.19 +2.0 3,324,214 + +2.5
Or see this region's nucleotide sequence