Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14925 and PS417_14930 are separated by 10 nucleotides PS417_14930 and PS417_14935 are separated by 28 nucleotides PS417_14935 and PS417_14940 are separated by 131 nucleotides
PS417_14925: PS417_14925 - 2-methylcitrate dehydratase, at 3,259,129 to 3,260,478
_14925
PS417_14930: PS417_14930 - acyl-CoA dehydrogenase, at 3,260,489 to 3,261,652
_14930
PS417_14935: PS417_14935 - transposase, at 3,261,681 to 3,262,499
_14935
PS417_14940: PS417_14940 - LysR family transcriptional regulator, at 3,262,631 to 3,263,521
_14940
Position (kb)
3260
3261
3262 Strain fitness (log2 ratio)
-2
-1
0
1 at 3259.794 kb on + strand, within PS417_14925 at 3259.795 kb on - strand, within PS417_14925 at 3259.909 kb on + strand, within PS417_14925 at 3260.322 kb on - strand, within PS417_14925 at 3260.529 kb on + strand at 3260.530 kb on - strand at 3260.604 kb on + strand at 3260.604 kb on + strand at 3260.605 kb on - strand at 3260.683 kb on - strand, within PS417_14930 at 3260.728 kb on - strand, within PS417_14930 at 3260.943 kb on + strand, within PS417_14930 at 3260.943 kb on + strand, within PS417_14930 at 3261.035 kb on - strand, within PS417_14930 at 3261.035 kb on - strand at 3261.213 kb on + strand, within PS417_14930 at 3261.213 kb on + strand at 3261.214 kb on - strand, within PS417_14930 at 3261.382 kb on - strand, within PS417_14930 at 3261.382 kb on - strand, within PS417_14930 at 3261.382 kb on - strand, within PS417_14930 at 3261.815 kb on - strand, within PS417_14935 at 3261.943 kb on + strand, within PS417_14935 at 3262.126 kb on + strand, within PS417_14935
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,259,794 + PS417_14925 0.49 -0.9 3,259,795 - PS417_14925 0.49 +0.7 3,259,909 + PS417_14925 0.58 +1.2 3,260,322 - PS417_14925 0.88 +0.1 3,260,529 + +0.4 3,260,530 - -1.0 3,260,604 + -0.0 3,260,604 + -2.3 3,260,605 - +0.5 3,260,683 - PS417_14930 0.17 +1.0 3,260,728 - PS417_14930 0.21 -1.4 3,260,943 + PS417_14930 0.39 -0.8 3,260,943 + PS417_14930 0.39 +1.5 3,261,035 - PS417_14930 0.47 +0.6 3,261,035 - -0.4 3,261,213 + PS417_14930 0.62 +0.0 3,261,213 + +1.1 3,261,214 - PS417_14930 0.62 +0.0 3,261,382 - PS417_14930 0.77 +1.3 3,261,382 - PS417_14930 0.77 +0.1 3,261,382 - PS417_14930 0.77 +0.8 3,261,815 - PS417_14935 0.16 +0.1 3,261,943 + PS417_14935 0.32 -0.3 3,262,126 + PS417_14935 0.54 -0.2
Or see this region's nucleotide sequence