Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01370 and PS417_01375 are separated by 14 nucleotides PS417_01375 and PS417_01380 are separated by 37 nucleotides PS417_01380 and PS417_01385 are separated by 519 nucleotides
PS417_01370: PS417_01370 - peptidase M48, at 298,541 to 300,118
_01370
PS417_01375: PS417_01375 - hypothetical protein, at 300,133 to 300,498
_01375
PS417_01380: PS417_01380 - nucleotide cyclase, at 300,536 to 301,876
_01380
PS417_01385: PS417_01385 - NAD-dependent dehydratase, at 302,396 to 303,328
_01385
Position (kb) 300
301
302 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 299.642 kb on + strand, within PS417_01370 at 299.642 kb on + strand, within PS417_01370 at 299.642 kb on + strand, within PS417_01370 at 299.643 kb on - strand, within PS417_01370 at 299.643 kb on - strand at 299.643 kb on - strand, within PS417_01370 at 299.841 kb on + strand, within PS417_01370 at 299.860 kb on + strand, within PS417_01370 at 299.914 kb on - strand, within PS417_01370 at 299.914 kb on - strand, within PS417_01370 at 299.955 kb on + strand, within PS417_01370 at 299.956 kb on - strand, within PS417_01370 at 299.956 kb on - strand, within PS417_01370 at 299.956 kb on - strand, within PS417_01370 at 299.958 kb on + strand, within PS417_01370 at 299.959 kb on - strand, within PS417_01370 at 299.977 kb on + strand at 299.985 kb on + strand at 299.986 kb on - strand at 299.986 kb on - strand at 299.986 kb on - strand at 299.986 kb on - strand at 300.105 kb on - strand at 300.134 kb on + strand at 300.135 kb on - strand at 300.217 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.321 kb on - strand at 300.381 kb on + strand, within PS417_01375 at 300.596 kb on + strand at 300.596 kb on + strand at 300.597 kb on - strand at 300.865 kb on - strand, within PS417_01380 at 301.072 kb on + strand, within PS417_01380 at 301.073 kb on - strand, within PS417_01380 at 301.073 kb on - strand, within PS417_01380 at 301.074 kb on + strand, within PS417_01380 at 301.075 kb on - strand, within PS417_01380 at 301.340 kb on + strand, within PS417_01380 at 301.526 kb on + strand, within PS417_01380 at 301.625 kb on + strand, within PS417_01380 at 301.856 kb on - strand at 301.966 kb on + strand at 301.967 kb on - strand at 301.967 kb on - strand at 301.967 kb on - strand at 301.967 kb on - strand at 302.029 kb on - strand at 302.081 kb on + strand at 302.081 kb on + strand at 302.082 kb on - strand at 302.341 kb on + strand at 302.382 kb on - strand at 302.404 kb on - strand at 302.438 kb on - strand at 302.477 kb on + strand at 302.512 kb on + strand, within PS417_01385 at 302.832 kb on - strand, within PS417_01385 at 302.859 kb on - strand, within PS417_01385 at 302.859 kb on - strand at 302.861 kb on + strand, within PS417_01385 at 302.874 kb on - strand, within PS417_01385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 299,642 + PS417_01370 0.70 +0.3 299,642 + PS417_01370 0.70 +0.8 299,642 + PS417_01370 0.70 +1.7 299,643 - PS417_01370 0.70 +0.3 299,643 - -0.3 299,643 - PS417_01370 0.70 +0.1 299,841 + PS417_01370 0.82 -0.6 299,860 + PS417_01370 0.84 +0.7 299,914 - PS417_01370 0.87 +0.1 299,914 - PS417_01370 0.87 +0.1 299,955 + PS417_01370 0.90 +0.8 299,956 - PS417_01370 0.90 -1.9 299,956 - PS417_01370 0.90 -0.3 299,956 - PS417_01370 0.90 +0.3 299,958 + PS417_01370 0.90 +0.2 299,959 - PS417_01370 0.90 -0.8 299,977 + +3.9 299,985 + -2.4 299,986 - -0.2 299,986 - +0.5 299,986 - -1.1 299,986 - +0.1 300,105 - +0.4 300,134 + -0.0 300,135 - +0.7 300,217 + PS417_01375 0.23 +2.0 300,320 + PS417_01375 0.51 +0.4 300,320 + PS417_01375 0.51 -0.5 300,320 + PS417_01375 0.51 -0.5 300,320 + PS417_01375 0.51 +0.4 300,321 - -1.8 300,381 + PS417_01375 0.68 +0.6 300,596 + +0.3 300,596 + -1.4 300,597 - -0.3 300,865 - PS417_01380 0.25 -0.9 301,072 + PS417_01380 0.40 -1.6 301,073 - PS417_01380 0.40 -1.4 301,073 - PS417_01380 0.40 -0.2 301,074 + PS417_01380 0.40 +0.2 301,075 - PS417_01380 0.40 -1.9 301,340 + PS417_01380 0.60 +0.7 301,526 + PS417_01380 0.74 -1.7 301,625 + PS417_01380 0.81 -0.1 301,856 - -1.2 301,966 + +2.0 301,967 - -0.2 301,967 - -2.5 301,967 - +0.1 301,967 - +0.5 302,029 - +1.0 302,081 + -1.4 302,081 + -2.1 302,082 - -1.8 302,341 + +1.6 302,382 - -1.8 302,404 - -0.9 302,438 - -0.1 302,477 + -1.0 302,512 + PS417_01385 0.12 -0.3 302,832 - PS417_01385 0.47 +0.4 302,859 - PS417_01385 0.50 -0.7 302,859 - -0.3 302,861 + PS417_01385 0.50 +1.8 302,874 - PS417_01385 0.51 +0.9
Or see this region's nucleotide sequence