Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01360 and PS417_01365 are separated by 20 nucleotides PS417_01365 and PS417_01370 are separated by 3 nucleotides PS417_01370 and PS417_01375 are separated by 14 nucleotides PS417_01375 and PS417_01380 are separated by 37 nucleotides
PS417_01360: PS417_01360 - 4-oxalocrotonate tautomerase, at 297,612 to 297,815
_01360
PS417_01365: PS417_01365 - 3-oxoacyl-ACP reductase, at 297,836 to 298,537
_01365
PS417_01370: PS417_01370 - peptidase M48, at 298,541 to 300,118
_01370
PS417_01375: PS417_01375 - hypothetical protein, at 300,133 to 300,498
_01375
PS417_01380: PS417_01380 - nucleotide cyclase, at 300,536 to 301,876
_01380
Position (kb) 298
299
300
301 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 297.555 kb on + strand at 297.555 kb on + strand at 297.555 kb on + strand at 297.651 kb on - strand, within PS417_01360 at 297.771 kb on + strand, within PS417_01360 at 298.100 kb on + strand at 298.154 kb on + strand, within PS417_01365 at 298.154 kb on + strand, within PS417_01365 at 298.509 kb on + strand at 298.510 kb on - strand at 298.579 kb on + strand at 298.579 kb on + strand at 298.656 kb on - strand at 298.860 kb on + strand, within PS417_01370 at 298.971 kb on + strand, within PS417_01370 at 298.971 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.122 kb on + strand, within PS417_01370 at 299.123 kb on - strand, within PS417_01370 at 299.455 kb on - strand, within PS417_01370 at 299.642 kb on + strand, within PS417_01370 at 299.642 kb on + strand, within PS417_01370 at 299.642 kb on + strand, within PS417_01370 at 299.643 kb on - strand, within PS417_01370 at 299.643 kb on - strand at 299.643 kb on - strand, within PS417_01370 at 299.841 kb on + strand, within PS417_01370 at 299.860 kb on + strand, within PS417_01370 at 299.914 kb on - strand, within PS417_01370 at 299.914 kb on - strand, within PS417_01370 at 299.955 kb on + strand, within PS417_01370 at 299.956 kb on - strand, within PS417_01370 at 299.956 kb on - strand, within PS417_01370 at 299.956 kb on - strand, within PS417_01370 at 299.958 kb on + strand, within PS417_01370 at 299.959 kb on - strand, within PS417_01370 at 299.977 kb on + strand at 299.985 kb on + strand at 299.986 kb on - strand at 299.986 kb on - strand at 299.986 kb on - strand at 299.986 kb on - strand at 300.105 kb on - strand at 300.134 kb on + strand at 300.135 kb on - strand at 300.217 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.320 kb on + strand, within PS417_01375 at 300.321 kb on - strand at 300.381 kb on + strand, within PS417_01375 at 300.596 kb on + strand at 300.596 kb on + strand at 300.597 kb on - strand at 300.865 kb on - strand, within PS417_01380 at 301.072 kb on + strand, within PS417_01380 at 301.073 kb on - strand, within PS417_01380 at 301.073 kb on - strand, within PS417_01380 at 301.074 kb on + strand, within PS417_01380 at 301.075 kb on - strand, within PS417_01380
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 297,555 + -0.7 297,555 + -1.8 297,555 + -2.0 297,651 - PS417_01360 0.19 -1.2 297,771 + PS417_01360 0.78 +0.1 298,100 + -0.3 298,154 + PS417_01365 0.45 -2.2 298,154 + PS417_01365 0.45 -0.5 298,509 + +0.7 298,510 - +0.6 298,579 + +1.2 298,579 + -2.2 298,656 - -0.4 298,860 + PS417_01370 0.20 +0.1 298,971 + PS417_01370 0.27 +1.4 298,971 + PS417_01370 0.27 +1.2 299,122 + PS417_01370 0.37 -0.2 299,122 + PS417_01370 0.37 +0.7 299,122 + PS417_01370 0.37 -0.3 299,122 + PS417_01370 0.37 -1.3 299,123 - PS417_01370 0.37 +0.4 299,455 - PS417_01370 0.58 +0.8 299,642 + PS417_01370 0.70 +0.3 299,642 + PS417_01370 0.70 +0.8 299,642 + PS417_01370 0.70 +1.7 299,643 - PS417_01370 0.70 +0.3 299,643 - -0.3 299,643 - PS417_01370 0.70 +0.1 299,841 + PS417_01370 0.82 -0.6 299,860 + PS417_01370 0.84 +0.7 299,914 - PS417_01370 0.87 +0.1 299,914 - PS417_01370 0.87 +0.1 299,955 + PS417_01370 0.90 +0.8 299,956 - PS417_01370 0.90 -1.9 299,956 - PS417_01370 0.90 -0.3 299,956 - PS417_01370 0.90 +0.3 299,958 + PS417_01370 0.90 +0.2 299,959 - PS417_01370 0.90 -0.8 299,977 + +3.9 299,985 + -2.4 299,986 - -0.2 299,986 - +0.5 299,986 - -1.1 299,986 - +0.1 300,105 - +0.4 300,134 + -0.0 300,135 - +0.7 300,217 + PS417_01375 0.23 +2.0 300,320 + PS417_01375 0.51 +0.4 300,320 + PS417_01375 0.51 -0.5 300,320 + PS417_01375 0.51 -0.5 300,320 + PS417_01375 0.51 +0.4 300,321 - -1.8 300,381 + PS417_01375 0.68 +0.6 300,596 + +0.3 300,596 + -1.4 300,597 - -0.3 300,865 - PS417_01380 0.25 -0.9 301,072 + PS417_01380 0.40 -1.6 301,073 - PS417_01380 0.40 -1.4 301,073 - PS417_01380 0.40 -0.2 301,074 + PS417_01380 0.40 +0.2 301,075 - PS417_01380 0.40 -1.9
Or see this region's nucleotide sequence