Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13655 and PS417_13660 are separated by 10 nucleotides PS417_13660 and PS417_13665 are separated by 109 nucleotides PS417_13665 and PS417_13670 overlap by 23 nucleotides
PS417_13655: PS417_13655 - xylulose kinase, at 3,004,799 to 3,006,289
_13655
PS417_13660: PS417_13660 - AsnC family transcriptional regulator, at 3,006,300 to 3,006,743
_13660
PS417_13665: PS417_13665 - multidrug DMT transporter permease, at 3,006,853 to 3,007,755
_13665
PS417_13670: PS417_13670 - protease, at 3,007,733 to 3,009,712
_13670
Position (kb)
3006
3007
3008 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3005.887 kb on + strand, within PS417_13655 at 3006.053 kb on + strand, within PS417_13655 at 3006.053 kb on + strand, within PS417_13655 at 3006.053 kb on + strand, within PS417_13655 at 3006.054 kb on - strand, within PS417_13655 at 3006.167 kb on + strand at 3006.374 kb on - strand, within PS417_13660 at 3006.593 kb on - strand, within PS417_13660 at 3006.593 kb on - strand, within PS417_13660 at 3006.754 kb on + strand at 3006.758 kb on - strand at 3006.824 kb on - strand at 3006.824 kb on - strand at 3007.154 kb on - strand, within PS417_13665 at 3007.170 kb on + strand, within PS417_13665 at 3007.171 kb on - strand, within PS417_13665 at 3007.171 kb on - strand, within PS417_13665 at 3007.303 kb on + strand, within PS417_13665 at 3007.345 kb on + strand, within PS417_13665 at 3007.528 kb on + strand, within PS417_13665 at 3007.528 kb on + strand, within PS417_13665 at 3007.529 kb on - strand, within PS417_13665 at 3007.529 kb on - strand, within PS417_13665 at 3007.595 kb on - strand, within PS417_13665 at 3007.741 kb on + strand at 3007.997 kb on + strand, within PS417_13670 at 3008.085 kb on + strand at 3008.085 kb on + strand, within PS417_13670 at 3008.086 kb on - strand, within PS417_13670 at 3008.283 kb on + strand, within PS417_13670 at 3008.323 kb on + strand, within PS417_13670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,005,887 + PS417_13655 0.73 +0.7 3,006,053 + PS417_13655 0.84 -0.2 3,006,053 + PS417_13655 0.84 +0.8 3,006,053 + PS417_13655 0.84 -1.8 3,006,054 - PS417_13655 0.84 +0.1 3,006,167 + +0.5 3,006,374 - PS417_13660 0.17 +0.6 3,006,593 - PS417_13660 0.66 -1.4 3,006,593 - PS417_13660 0.66 -0.1 3,006,754 + -0.4 3,006,758 - +0.1 3,006,824 - -0.5 3,006,824 - -0.5 3,007,154 - PS417_13665 0.33 +0.1 3,007,170 + PS417_13665 0.35 +0.4 3,007,171 - PS417_13665 0.35 -0.6 3,007,171 - PS417_13665 0.35 +0.3 3,007,303 + PS417_13665 0.50 -0.2 3,007,345 + PS417_13665 0.54 +0.7 3,007,528 + PS417_13665 0.75 -0.5 3,007,528 + PS417_13665 0.75 +0.1 3,007,529 - PS417_13665 0.75 -0.8 3,007,529 - PS417_13665 0.75 +3.9 3,007,595 - PS417_13665 0.82 +1.1 3,007,741 + +0.6 3,007,997 + PS417_13670 0.13 -1.7 3,008,085 + +3.4 3,008,085 + PS417_13670 0.18 -2.1 3,008,086 - PS417_13670 0.18 -0.6 3,008,283 + PS417_13670 0.28 -0.4 3,008,323 + PS417_13670 0.30 +2.6
Or see this region's nucleotide sequence