Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13430 and PS417_13435 are separated by 132 nucleotides PS417_13435 and PS417_13440 are separated by 200 nucleotides
PS417_13430: PS417_13430 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,953,973 to 2,955,052
_13430
PS417_13435: PS417_13435 - LuxR family transcriptional regulator, at 2,955,185 to 2,955,970
_13435
PS417_13440: PS417_13440 - amino acid permease, at 2,956,171 to 2,957,535
_13440
Position (kb)
2955
2956 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2954.344 kb on - strand, within PS417_13430 at 2954.519 kb on + strand, within PS417_13430 at 2954.520 kb on - strand, within PS417_13430 at 2954.520 kb on - strand, within PS417_13430 at 2954.559 kb on - strand, within PS417_13430 at 2954.597 kb on + strand, within PS417_13430 at 2954.598 kb on - strand, within PS417_13430 at 2954.598 kb on - strand, within PS417_13430 at 2954.606 kb on + strand, within PS417_13430 at 2954.624 kb on + strand, within PS417_13430 at 2954.624 kb on + strand, within PS417_13430 at 2954.735 kb on + strand, within PS417_13430 at 2954.817 kb on - strand, within PS417_13430 at 2954.832 kb on - strand at 2954.870 kb on + strand, within PS417_13430 at 2954.870 kb on + strand, within PS417_13430 at 2954.871 kb on - strand, within PS417_13430 at 2954.871 kb on - strand, within PS417_13430 at 2954.871 kb on - strand, within PS417_13430 at 2954.880 kb on - strand, within PS417_13430 at 2955.047 kb on - strand at 2955.050 kb on + strand at 2955.051 kb on - strand at 2955.051 kb on - strand at 2955.051 kb on - strand at 2955.131 kb on - strand at 2955.172 kb on + strand at 2955.172 kb on + strand at 2955.221 kb on - strand at 2955.627 kb on + strand, within PS417_13435 at 2955.627 kb on + strand, within PS417_13435 at 2955.627 kb on + strand, within PS417_13435 at 2955.639 kb on + strand, within PS417_13435 at 2955.685 kb on - strand, within PS417_13435 at 2955.753 kb on + strand, within PS417_13435 at 2955.754 kb on - strand, within PS417_13435 at 2955.754 kb on - strand, within PS417_13435 at 2955.832 kb on - strand, within PS417_13435 at 2956.047 kb on - strand at 2956.105 kb on + strand at 2956.106 kb on - strand at 2956.171 kb on - strand at 2956.171 kb on - strand at 2956.370 kb on - strand, within PS417_13440 at 2956.411 kb on + strand, within PS417_13440 at 2956.411 kb on + strand, within PS417_13440 at 2956.412 kb on - strand, within PS417_13440 at 2956.522 kb on + strand, within PS417_13440 at 2956.522 kb on + strand, within PS417_13440 at 2956.592 kb on - strand, within PS417_13440 at 2956.810 kb on + strand, within PS417_13440 at 2956.810 kb on + strand, within PS417_13440 at 2956.811 kb on - strand, within PS417_13440 at 2956.819 kb on + strand, within PS417_13440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,954,344 - PS417_13430 0.34 +0.9 2,954,519 + PS417_13430 0.51 -0.8 2,954,520 - PS417_13430 0.51 -0.2 2,954,520 - PS417_13430 0.51 +2.0 2,954,559 - PS417_13430 0.54 +1.0 2,954,597 + PS417_13430 0.58 -0.0 2,954,598 - PS417_13430 0.58 +0.3 2,954,598 - PS417_13430 0.58 +1.4 2,954,606 + PS417_13430 0.59 +0.0 2,954,624 + PS417_13430 0.60 +0.0 2,954,624 + PS417_13430 0.60 -1.1 2,954,735 + PS417_13430 0.71 -1.5 2,954,817 - PS417_13430 0.78 +0.9 2,954,832 - +1.2 2,954,870 + PS417_13430 0.83 -0.2 2,954,870 + PS417_13430 0.83 -1.2 2,954,871 - PS417_13430 0.83 +1.1 2,954,871 - PS417_13430 0.83 -0.2 2,954,871 - PS417_13430 0.83 +0.4 2,954,880 - PS417_13430 0.84 -0.4 2,955,047 - -0.4 2,955,050 + -0.2 2,955,051 - -0.5 2,955,051 - +0.2 2,955,051 - +0.3 2,955,131 - +0.2 2,955,172 + +1.1 2,955,172 + -1.7 2,955,221 - -1.4 2,955,627 + PS417_13435 0.56 +1.1 2,955,627 + PS417_13435 0.56 -0.7 2,955,627 + PS417_13435 0.56 -1.9 2,955,639 + PS417_13435 0.58 +0.4 2,955,685 - PS417_13435 0.64 -0.3 2,955,753 + PS417_13435 0.72 +0.4 2,955,754 - PS417_13435 0.72 -2.0 2,955,754 - PS417_13435 0.72 +1.1 2,955,832 - PS417_13435 0.82 +3.0 2,956,047 - -0.5 2,956,105 + -0.0 2,956,106 - -0.3 2,956,171 - +1.1 2,956,171 - -0.4 2,956,370 - PS417_13440 0.15 -0.0 2,956,411 + PS417_13440 0.18 +0.8 2,956,411 + PS417_13440 0.18 -0.4 2,956,412 - PS417_13440 0.18 +0.8 2,956,522 + PS417_13440 0.26 +0.0 2,956,522 + PS417_13440 0.26 -0.5 2,956,592 - PS417_13440 0.31 -0.2 2,956,810 + PS417_13440 0.47 +1.7 2,956,810 + PS417_13440 0.47 -0.0 2,956,811 - PS417_13440 0.47 -0.3 2,956,819 + PS417_13440 0.47 +0.1
Or see this region's nucleotide sequence