Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13305 and PS417_13310 overlap by 8 nucleotides PS417_13310 and PS417_13315 are separated by 9 nucleotides PS417_13315 and PS417_13320 overlap by 4 nucleotides
PS417_13305: PS417_13305 - hypothetical protein, at 2,924,543 to 2,924,863
_13305
PS417_13310: PS417_13310 - sugar transporter, at 2,924,856 to 2,925,728
_13310
PS417_13315: PS417_13315 - hypothetical protein, at 2,925,738 to 2,927,459
_13315
PS417_13320: PS417_13320 - L-alanine-DL-glutamate epimerase, at 2,927,456 to 2,928,649
_13320
Position (kb)
2925
2926
2927
2928 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2924.989 kb on + strand, within PS417_13310 at 2924.989 kb on + strand, within PS417_13310 at 2924.990 kb on - strand at 2925.119 kb on - strand, within PS417_13310 at 2925.163 kb on + strand at 2925.211 kb on + strand, within PS417_13310 at 2925.212 kb on - strand, within PS417_13310 at 2925.274 kb on + strand, within PS417_13310 at 2925.275 kb on - strand, within PS417_13310 at 2925.275 kb on - strand, within PS417_13310 at 2925.364 kb on - strand, within PS417_13310 at 2925.578 kb on - strand, within PS417_13310 at 2925.688 kb on + strand at 2925.787 kb on + strand at 2925.788 kb on - strand at 2925.788 kb on - strand at 2925.788 kb on - strand at 2925.850 kb on + strand at 2925.983 kb on - strand, within PS417_13315 at 2925.983 kb on - strand, within PS417_13315 at 2926.180 kb on + strand, within PS417_13315 at 2926.181 kb on - strand, within PS417_13315 at 2926.969 kb on + strand, within PS417_13315 at 2926.970 kb on - strand, within PS417_13315 at 2926.970 kb on - strand, within PS417_13315 at 2927.272 kb on + strand, within PS417_13315 at 2927.272 kb on + strand, within PS417_13315 at 2927.273 kb on - strand, within PS417_13315 at 2927.273 kb on - strand, within PS417_13315 at 2927.404 kb on + strand at 2927.405 kb on - strand at 2927.452 kb on + strand at 2927.453 kb on - strand at 2927.600 kb on - strand, within PS417_13320 at 2927.748 kb on + strand, within PS417_13320 at 2928.270 kb on + strand, within PS417_13320 at 2928.271 kb on - strand, within PS417_13320 at 2928.271 kb on - strand, within PS417_13320 at 2928.271 kb on - strand at 2928.387 kb on + strand, within PS417_13320 at 2928.387 kb on + strand, within PS417_13320
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,924,989 + PS417_13310 0.15 +0.3 2,924,989 + PS417_13310 0.15 +0.7 2,924,990 - -0.2 2,925,119 - PS417_13310 0.30 -0.2 2,925,163 + +1.8 2,925,211 + PS417_13310 0.41 -0.3 2,925,212 - PS417_13310 0.41 -0.5 2,925,274 + PS417_13310 0.48 +0.1 2,925,275 - PS417_13310 0.48 +0.1 2,925,275 - PS417_13310 0.48 -1.6 2,925,364 - PS417_13310 0.58 +0.4 2,925,578 - PS417_13310 0.83 -0.1 2,925,688 + +0.3 2,925,787 + -1.0 2,925,788 - -0.4 2,925,788 - -0.6 2,925,788 - +0.1 2,925,850 + -1.8 2,925,983 - PS417_13315 0.14 -0.3 2,925,983 - PS417_13315 0.14 +0.2 2,926,180 + PS417_13315 0.26 +0.9 2,926,181 - PS417_13315 0.26 +0.6 2,926,969 + PS417_13315 0.71 +0.1 2,926,970 - PS417_13315 0.72 -0.9 2,926,970 - PS417_13315 0.72 -0.3 2,927,272 + PS417_13315 0.89 -0.8 2,927,272 + PS417_13315 0.89 -1.5 2,927,273 - PS417_13315 0.89 -0.0 2,927,273 - PS417_13315 0.89 +0.5 2,927,404 + -0.8 2,927,405 - -1.2 2,927,452 + -2.0 2,927,453 - -1.2 2,927,600 - PS417_13320 0.12 +2.1 2,927,748 + PS417_13320 0.24 +0.5 2,928,270 + PS417_13320 0.68 -0.4 2,928,271 - PS417_13320 0.68 -0.1 2,928,271 - PS417_13320 0.68 +0.2 2,928,271 - +2.5 2,928,387 + PS417_13320 0.78 -0.7 2,928,387 + PS417_13320 0.78 +0.6
Or see this region's nucleotide sequence