Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12610 and PS417_12615 are separated by 170 nucleotides PS417_12615 and PS417_12620 are separated by 138 nucleotides PS417_12620 and PS417_12625 are separated by 91 nucleotides
PS417_12610: PS417_12610 - RNA polymerase sigma factor, at 2,745,135 to 2,745,650
_12610
PS417_12615: PS417_12615 - hypothetical protein, at 2,745,821 to 2,746,342
_12615
PS417_12620: PS417_12620 - SAM-dependent methyltransferase, at 2,746,481 to 2,747,251
_12620
PS417_12625: PS417_12625 - membrane protein, at 2,747,343 to 2,748,128
_12625
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2745.583 kb on + strand at 2745.584 kb on - strand, within PS417_12610 at 2745.652 kb on + strand at 2745.652 kb on + strand at 2745.652 kb on + strand at 2745.653 kb on - strand at 2745.653 kb on - strand at 2745.653 kb on - strand at 2745.684 kb on - strand at 2745.684 kb on - strand at 2745.684 kb on - strand at 2745.777 kb on + strand at 2745.777 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.784 kb on + strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.785 kb on - strand at 2745.808 kb on + strand at 2745.808 kb on + strand at 2745.943 kb on - strand, within PS417_12615 at 2746.095 kb on + strand, within PS417_12615 at 2746.095 kb on + strand, within PS417_12615 at 2746.198 kb on - strand, within PS417_12615 at 2746.198 kb on - strand, within PS417_12615 at 2746.312 kb on + strand at 2746.334 kb on - strand at 2746.347 kb on - strand at 2746.362 kb on + strand at 2746.491 kb on + strand at 2746.491 kb on + strand at 2746.492 kb on - strand at 2746.492 kb on - strand at 2746.492 kb on - strand at 2746.946 kb on - strand, within PS417_12620 at 2747.035 kb on - strand, within PS417_12620 at 2747.035 kb on - strand, within PS417_12620 at 2747.228 kb on + strand at 2747.228 kb on + strand at 2747.228 kb on + strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.229 kb on - strand at 2747.278 kb on - strand at 2747.280 kb on - strand at 2747.323 kb on + strand at 2747.335 kb on + strand at 2747.336 kb on - strand at 2747.336 kb on - strand at 2747.394 kb on - strand at 2747.493 kb on + strand, within PS417_12625 at 2747.546 kb on + strand, within PS417_12625 at 2747.677 kb on + strand, within PS417_12625 at 2747.757 kb on + strand, within PS417_12625 at 2747.757 kb on + strand, within PS417_12625 at 2747.758 kb on - strand, within PS417_12625 at 2747.874 kb on - strand at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.030 kb on + strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.031 kb on - strand, within PS417_12625 at 2748.044 kb on + strand, within PS417_12625 at 2748.045 kb on - strand, within PS417_12625 at 2748.111 kb on + strand at 2748.224 kb on + strand at 2748.225 kb on - strand at 2748.233 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,745,583 + +2.7 2,745,584 - PS417_12610 0.87 -0.2 2,745,652 + -1.7 2,745,652 + +0.3 2,745,652 + +0.2 2,745,653 - -0.4 2,745,653 - -1.1 2,745,653 - -0.0 2,745,684 - -0.8 2,745,684 - -0.7 2,745,684 - +0.2 2,745,777 + +0.2 2,745,777 + -1.1 2,745,784 + -0.3 2,745,784 + -1.2 2,745,784 + +0.2 2,745,784 + +0.2 2,745,784 + +0.1 2,745,784 + -0.1 2,745,785 - -0.8 2,745,785 - -0.5 2,745,785 - -1.0 2,745,785 - -0.7 2,745,785 - +0.2 2,745,785 - -0.4 2,745,785 - +0.2 2,745,808 + +0.2 2,745,808 + +0.4 2,745,943 - PS417_12615 0.23 -0.2 2,746,095 + PS417_12615 0.52 -0.9 2,746,095 + PS417_12615 0.52 +0.1 2,746,198 - PS417_12615 0.72 -0.2 2,746,198 - PS417_12615 0.72 -0.6 2,746,312 + -1.4 2,746,334 - -0.8 2,746,347 - +0.6 2,746,362 + -1.4 2,746,491 + -0.4 2,746,491 + +0.9 2,746,492 - -0.6 2,746,492 - +1.0 2,746,492 - -1.4 2,746,946 - PS417_12620 0.60 +2.5 2,747,035 - PS417_12620 0.72 -0.3 2,747,035 - PS417_12620 0.72 +1.7 2,747,228 + -0.6 2,747,228 + -0.1 2,747,228 + +0.5 2,747,229 - -0.8 2,747,229 - +0.0 2,747,229 - -0.2 2,747,229 - -0.3 2,747,278 - +1.3 2,747,280 - -0.4 2,747,323 + -0.5 2,747,335 + -0.6 2,747,336 - -0.2 2,747,336 - -2.0 2,747,394 - -0.7 2,747,493 + PS417_12625 0.19 +0.0 2,747,546 + PS417_12625 0.26 +2.0 2,747,677 + PS417_12625 0.42 -0.1 2,747,757 + PS417_12625 0.53 -0.3 2,747,757 + PS417_12625 0.53 +0.2 2,747,758 - PS417_12625 0.53 +0.5 2,747,874 - -1.6 2,748,030 + PS417_12625 0.87 +1.0 2,748,030 + PS417_12625 0.87 +0.0 2,748,030 + PS417_12625 0.87 +0.0 2,748,030 + PS417_12625 0.87 -0.5 2,748,030 + PS417_12625 0.87 -1.3 2,748,031 - PS417_12625 0.88 +1.5 2,748,031 - PS417_12625 0.88 -0.7 2,748,031 - PS417_12625 0.88 +0.2 2,748,031 - PS417_12625 0.88 +0.0 2,748,044 + PS417_12625 0.89 -0.6 2,748,045 - PS417_12625 0.89 -0.4 2,748,111 + +0.2 2,748,224 + -0.6 2,748,225 - -0.0 2,748,233 + -0.6
Or see this region's nucleotide sequence