Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01215 and PS417_01220 are separated by 28 nucleotides PS417_01220 and PS417_01230 are separated by 610 nucleotides PS417_01230 and PS417_01235 overlap by 28 nucleotides PS417_01235 and PS417_01245 are separated by 345 nucleotides
PS417_01215: PS417_01215 - membrane protein, at 267,031 to 267,882
_01215
PS417_01220: PS417_01220 - hypothetical protein, at 267,911 to 268,267
_01220
PS417_01230: PS417_01230 - hypothetical protein, at 268,878 to 269,123
_01230
PS417_01235: PS417_01235 - cell division protein Fic, at 269,096 to 269,695
_01235
PS417_01245: PS417_01245 - hypothetical protein, at 270,041 to 271,216
_01245
Position (kb) 268
269
270 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 267.897 kb on - strand at 267.897 kb on - strand at 267.897 kb on - strand at 267.897 kb on - strand at 267.966 kb on + strand, within PS417_01220 at 267.967 kb on - strand, within PS417_01220 at 267.967 kb on - strand, within PS417_01220 at 268.054 kb on - strand, within PS417_01220 at 268.059 kb on + strand, within PS417_01220 at 268.059 kb on + strand, within PS417_01220 at 268.059 kb on + strand, within PS417_01220 at 268.060 kb on - strand, within PS417_01220 at 268.060 kb on - strand, within PS417_01220 at 268.060 kb on - strand, within PS417_01220 at 268.358 kb on + strand at 268.358 kb on + strand at 268.359 kb on - strand at 268.359 kb on - strand at 268.397 kb on + strand at 268.397 kb on + strand at 268.397 kb on + strand at 268.397 kb on + strand at 268.397 kb on + strand at 268.401 kb on + strand at 268.401 kb on + strand at 268.401 kb on + strand at 268.401 kb on + strand at 268.402 kb on - strand at 268.402 kb on - strand at 268.402 kb on - strand at 268.406 kb on + strand at 268.406 kb on + strand at 268.409 kb on + strand at 268.409 kb on + strand at 268.409 kb on + strand at 268.423 kb on + strand at 268.500 kb on + strand at 268.501 kb on - strand at 268.501 kb on - strand at 268.501 kb on - strand at 268.504 kb on + strand at 268.504 kb on + strand at 268.598 kb on + strand at 268.598 kb on + strand at 268.599 kb on - strand at 268.599 kb on - strand at 268.599 kb on - strand at 268.648 kb on + strand at 268.652 kb on + strand at 268.652 kb on + strand at 268.652 kb on + strand at 268.652 kb on + strand at 268.652 kb on + strand at 268.653 kb on - strand at 268.820 kb on + strand at 268.820 kb on + strand at 269.011 kb on - strand, within PS417_01230 at 269.011 kb on - strand, within PS417_01230 at 269.014 kb on + strand, within PS417_01230 at 269.015 kb on - strand, within PS417_01230 at 269.015 kb on - strand, within PS417_01230 at 269.078 kb on + strand, within PS417_01230 at 269.119 kb on + strand at 269.120 kb on - strand at 269.173 kb on + strand, within PS417_01235 at 269.173 kb on + strand, within PS417_01235 at 269.361 kb on + strand, within PS417_01235 at 269.361 kb on + strand, within PS417_01235 at 269.362 kb on - strand, within PS417_01235 at 269.485 kb on - strand, within PS417_01235 at 269.512 kb on + strand, within PS417_01235 at 269.513 kb on - strand, within PS417_01235 at 269.513 kb on - strand, within PS417_01235 at 269.513 kb on - strand, within PS417_01235 at 269.535 kb on + strand, within PS417_01235 at 269.536 kb on - strand, within PS417_01235 at 269.828 kb on + strand at 269.873 kb on + strand at 269.899 kb on + strand at 269.949 kb on + strand at 269.982 kb on + strand at 269.983 kb on - strand at 269.983 kb on - strand at 269.983 kb on - strand at 270.004 kb on + strand at 270.005 kb on - strand at 270.022 kb on + strand at 270.023 kb on - strand at 270.023 kb on - strand at 270.026 kb on + strand at 270.026 kb on + strand at 270.027 kb on - strand at 270.027 kb on - strand at 270.036 kb on + strand at 270.037 kb on - strand at 270.037 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 267,897 - -0.7 267,897 - +0.6 267,897 - -0.4 267,897 - +0.2 267,966 + PS417_01220 0.15 +0.7 267,967 - PS417_01220 0.16 -0.2 267,967 - PS417_01220 0.16 -0.0 268,054 - PS417_01220 0.40 +2.1 268,059 + PS417_01220 0.41 -1.0 268,059 + PS417_01220 0.41 -0.2 268,059 + PS417_01220 0.41 +0.1 268,060 - PS417_01220 0.42 +0.5 268,060 - PS417_01220 0.42 -0.6 268,060 - PS417_01220 0.42 +0.5 268,358 + +0.4 268,358 + -1.2 268,359 - +3.3 268,359 - +0.1 268,397 + +1.7 268,397 + +0.1 268,397 + +1.4 268,397 + +0.6 268,397 + -0.2 268,401 + -0.3 268,401 + -0.3 268,401 + +2.3 268,401 + -2.3 268,402 - -1.0 268,402 - +0.1 268,402 - +0.6 268,406 + -0.1 268,406 + +0.9 268,409 + -0.5 268,409 + +0.4 268,409 + -2.0 268,423 + +0.5 268,500 + -0.9 268,501 - +1.2 268,501 - +0.9 268,501 - +0.3 268,504 + -0.0 268,504 + -1.0 268,598 + +0.6 268,598 + +0.8 268,599 - +2.9 268,599 - -0.1 268,599 - +0.7 268,648 + -0.6 268,652 + +0.3 268,652 + +0.6 268,652 + -1.3 268,652 + -0.5 268,652 + -0.0 268,653 - +2.5 268,820 + -0.1 268,820 + +0.9 269,011 - PS417_01230 0.54 +0.5 269,011 - PS417_01230 0.54 +0.6 269,014 + PS417_01230 0.55 +1.4 269,015 - PS417_01230 0.56 +0.6 269,015 - PS417_01230 0.56 -0.3 269,078 + PS417_01230 0.81 +0.3 269,119 + -1.5 269,120 - +0.2 269,173 + PS417_01235 0.13 +1.5 269,173 + PS417_01235 0.13 +0.4 269,361 + PS417_01235 0.44 +0.3 269,361 + PS417_01235 0.44 +0.6 269,362 - PS417_01235 0.44 +1.4 269,485 - PS417_01235 0.65 +0.8 269,512 + PS417_01235 0.69 -0.3 269,513 - PS417_01235 0.69 -0.1 269,513 - PS417_01235 0.69 +1.7 269,513 - PS417_01235 0.69 -1.6 269,535 + PS417_01235 0.73 -0.2 269,536 - PS417_01235 0.73 -0.2 269,828 + -1.6 269,873 + -0.3 269,899 + -0.3 269,949 + +0.4 269,982 + -1.4 269,983 - -0.8 269,983 - +0.0 269,983 - +0.8 270,004 + +0.2 270,005 - -2.7 270,022 + +0.5 270,023 - +0.7 270,023 - -0.3 270,026 + -0.8 270,026 + +0.4 270,027 - -0.4 270,027 - -0.0 270,036 + +0.3 270,037 - +2.0 270,037 - +0.4
Or see this region's nucleotide sequence