Strain Fitness in Pseudomonas simiae WCS417 around PS417_12275

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_12270 and PS417_12275 are separated by 92 nucleotidesPS417_12275 and PS417_12280 are separated by 71 nucleotidesPS417_12280 and PS417_12285 are separated by 97 nucleotides PS417_12270: PS417_12270 - pyruvate dehydrogenase, at 2,665,309 to 2,667,018 _12270 PS417_12275: PS417_12275 - 2,4-dienoyl-CoA reductase, at 2,667,111 to 2,668,343 _12275 PS417_12280: PS417_12280 - MerR family transcriptional regulator, at 2,668,415 to 2,668,819 _12280 PS417_12285: PS417_12285 - oxidoreductase, at 2,668,917 to 2,669,414 _12285 Position (kb) 2667 2668 2669Strain fitness (log2 ratio) -2 -1 0 1at 2666.341 kb on + strand, within PS417_12270at 2667.181 kb on - strandat 2667.191 kb on + strandat 2667.411 kb on + strand, within PS417_12275at 2667.412 kb on - strand, within PS417_12275at 2667.505 kb on + strand, within PS417_12275at 2667.506 kb on - strand, within PS417_12275at 2667.824 kb on - strand, within PS417_12275at 2668.216 kb on + strand, within PS417_12275at 2668.216 kb on + strand, within PS417_12275at 2668.217 kb on - strand, within PS417_12275at 2668.217 kb on - strand, within PS417_12275at 2668.217 kb on - strand, within PS417_12275at 2668.289 kb on + strandat 2668.377 kb on - strandat 2668.382 kb on + strandat 2668.395 kb on - strandat 2668.472 kb on + strand, within PS417_12280at 2668.529 kb on + strand, within PS417_12280at 2668.791 kb on + strandat 2668.791 kb on + strandat 2668.966 kb on + strandat 2668.966 kb on + strandat 2668.966 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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2,666,341 + PS417_12270 0.60 -0.8
2,667,181 - +0.1
2,667,191 + +0.9
2,667,411 + PS417_12275 0.24 +0.4
2,667,412 - PS417_12275 0.24 +0.1
2,667,505 + PS417_12275 0.32 -0.8
2,667,506 - PS417_12275 0.32 +0.7
2,667,824 - PS417_12275 0.58 -0.1
2,668,216 + PS417_12275 0.90 -0.1
2,668,216 + PS417_12275 0.90 +0.8
2,668,217 - PS417_12275 0.90 +0.4
2,668,217 - PS417_12275 0.90 +1.1
2,668,217 - PS417_12275 0.90 +0.0
2,668,289 + -0.6
2,668,377 - -0.5
2,668,382 + -1.4
2,668,395 - +0.2
2,668,472 + PS417_12280 0.14 +0.2
2,668,529 + PS417_12280 0.28 -0.0
2,668,791 + -0.4
2,668,791 + +0.2
2,668,966 + -1.4
2,668,966 + -1.2
2,668,966 + -2.1

Or see this region's nucleotide sequence