Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12240 and PS417_12245 are separated by 20 nucleotides PS417_12245 and PS417_12250 overlap by 11 nucleotides PS417_12250 and PS417_12255 are separated by 109 nucleotides PS417_12255 and PS417_12260 are separated by 60 nucleotides PS417_12260 and PS417_12265 are separated by 227 nucleotides
PS417_12240: PS417_12240 - lysine decarboxylase, at 2,659,927 to 2,662,182
_12240
PS417_12245: PS417_12245 - GNAT family acetyltransferase, at 2,662,203 to 2,662,757
_12245
PS417_12250: PS417_12250 - LysR family transcriptional regulator, at 2,662,747 to 2,663,667
_12250
PS417_12255: PS417_12255 - hypothetical protein, at 2,663,777 to 2,664,121
_12255
PS417_12260: PS417_12260 - hypothetical protein, at 2,664,182 to 2,664,418
_12260
PS417_12265: PS417_12265 - ACP phosphodiesterase, at 2,664,646 to 2,665,203
_12265
Position (kb)
2662
2663
2664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2661.826 kb on + strand, within PS417_12240 at 2661.866 kb on - strand, within PS417_12240 at 2661.908 kb on - strand, within PS417_12240 at 2662.157 kb on - strand at 2662.157 kb on - strand at 2662.190 kb on + strand at 2662.190 kb on + strand at 2662.190 kb on + strand at 2662.216 kb on - strand at 2662.261 kb on - strand, within PS417_12245 at 2662.351 kb on + strand, within PS417_12245 at 2662.464 kb on - strand, within PS417_12245 at 2662.464 kb on - strand, within PS417_12245 at 2662.647 kb on + strand, within PS417_12245 at 2662.662 kb on + strand at 2662.755 kb on + strand at 2662.868 kb on + strand, within PS417_12250 at 2662.997 kb on + strand, within PS417_12250 at 2662.998 kb on - strand, within PS417_12250 at 2663.129 kb on + strand, within PS417_12250 at 2663.130 kb on - strand, within PS417_12250 at 2663.130 kb on - strand, within PS417_12250 at 2663.333 kb on - strand, within PS417_12250 at 2663.573 kb on - strand, within PS417_12250 at 2663.573 kb on - strand, within PS417_12250 at 2663.679 kb on - strand at 2663.679 kb on - strand at 2663.703 kb on + strand at 2663.703 kb on + strand at 2663.703 kb on + strand at 2663.777 kb on - strand at 2663.856 kb on - strand, within PS417_12255 at 2664.026 kb on + strand, within PS417_12255 at 2664.026 kb on + strand, within PS417_12255 at 2664.026 kb on + strand, within PS417_12255 at 2664.183 kb on + strand at 2664.184 kb on - strand at 2664.291 kb on + strand, within PS417_12260 at 2664.292 kb on - strand, within PS417_12260 at 2664.301 kb on + strand, within PS417_12260 at 2664.364 kb on - strand, within PS417_12260 at 2664.364 kb on - strand, within PS417_12260 at 2664.407 kb on + strand at 2664.408 kb on - strand at 2664.408 kb on - strand at 2664.408 kb on - strand at 2664.440 kb on - strand at 2664.448 kb on + strand at 2664.448 kb on + strand at 2664.448 kb on + strand at 2664.448 kb on + strand at 2664.483 kb on - strand at 2664.483 kb on - strand at 2664.487 kb on + strand at 2664.488 kb on - strand at 2664.488 kb on - strand at 2664.491 kb on + strand at 2664.507 kb on + strand at 2664.508 kb on - strand at 2664.543 kb on + strand at 2664.544 kb on - strand at 2664.570 kb on - strand at 2664.605 kb on + strand at 2664.605 kb on + strand at 2664.605 kb on + strand at 2664.607 kb on + strand at 2664.608 kb on - strand at 2664.611 kb on + strand at 2664.612 kb on - strand at 2664.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,661,826 + PS417_12240 0.84 -0.2 2,661,866 - PS417_12240 0.86 +0.5 2,661,908 - PS417_12240 0.88 -0.3 2,662,157 - +0.4 2,662,157 - +0.3 2,662,190 + -0.6 2,662,190 + -0.6 2,662,190 + -1.6 2,662,216 - -0.3 2,662,261 - PS417_12245 0.10 -0.2 2,662,351 + PS417_12245 0.27 +0.5 2,662,464 - PS417_12245 0.47 -1.2 2,662,464 - PS417_12245 0.47 -0.3 2,662,647 + PS417_12245 0.80 -2.2 2,662,662 + +1.1 2,662,755 + +1.0 2,662,868 + PS417_12250 0.13 +0.2 2,662,997 + PS417_12250 0.27 +1.1 2,662,998 - PS417_12250 0.27 -0.1 2,663,129 + PS417_12250 0.41 -1.6 2,663,130 - PS417_12250 0.42 +1.8 2,663,130 - PS417_12250 0.42 +0.4 2,663,333 - PS417_12250 0.64 +2.5 2,663,573 - PS417_12250 0.90 +0.1 2,663,573 - PS417_12250 0.90 +0.2 2,663,679 - +0.3 2,663,679 - +1.4 2,663,703 + +1.5 2,663,703 + +0.6 2,663,703 + +1.4 2,663,777 - +0.8 2,663,856 - PS417_12255 0.23 -0.1 2,664,026 + PS417_12255 0.72 +0.3 2,664,026 + PS417_12255 0.72 +0.3 2,664,026 + PS417_12255 0.72 -2.9 2,664,183 + +0.3 2,664,184 - +0.3 2,664,291 + PS417_12260 0.46 +0.1 2,664,292 - PS417_12260 0.46 -0.1 2,664,301 + PS417_12260 0.50 -2.1 2,664,364 - PS417_12260 0.77 -2.0 2,664,364 - PS417_12260 0.77 -1.0 2,664,407 + -2.7 2,664,408 - +0.2 2,664,408 - +0.8 2,664,408 - +0.9 2,664,440 - -0.5 2,664,448 + -1.2 2,664,448 + -0.6 2,664,448 + -1.3 2,664,448 + +0.1 2,664,483 - -1.1 2,664,483 - -0.4 2,664,487 + +0.6 2,664,488 - -1.0 2,664,488 - +1.1 2,664,491 + +1.7 2,664,507 + +1.2 2,664,508 - +0.3 2,664,543 + -0.8 2,664,544 - -0.3 2,664,570 - -0.2 2,664,605 + +1.4 2,664,605 + +1.7 2,664,605 + -0.5 2,664,607 + +0.0 2,664,608 - +0.0 2,664,611 + +0.7 2,664,612 - -0.8 2,664,612 - +1.2
Or see this region's nucleotide sequence