Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12175 and PS417_12180 overlap by 1 nucleotides PS417_12180 and PS417_12185 are separated by 130 nucleotides PS417_12185 and PS417_12190 are separated by 121 nucleotides
PS417_12175: PS417_12175 - peptide ABC transporter permease, at 2,644,381 to 2,645,442
_12175
PS417_12180: PS417_12180 - ABC transporter substrate-binding protein, at 2,645,442 to 2,647,301
_12180
PS417_12185: PS417_12185 - peptidylprolyl isomerase, at 2,647,432 to 2,647,713
_12185
PS417_12190: PS417_12190 - microcin ABC transporter ATP-binding protein, at 2,647,835 to 2,649,430
_12190
Position (kb)
2645
2646
2647
2648 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2644.591 kb on + strand, within PS417_12175 at 2644.648 kb on + strand, within PS417_12175 at 2644.658 kb on + strand, within PS417_12175 at 2644.659 kb on - strand, within PS417_12175 at 2645.040 kb on - strand, within PS417_12175 at 2645.154 kb on - strand, within PS417_12175 at 2645.605 kb on + strand at 2645.605 kb on + strand at 2645.605 kb on + strand at 2645.606 kb on - strand at 2645.623 kb on + strand at 2645.624 kb on - strand at 2645.624 kb on - strand at 2645.624 kb on - strand at 2645.741 kb on - strand, within PS417_12180 at 2645.741 kb on - strand, within PS417_12180 at 2645.782 kb on + strand, within PS417_12180 at 2645.782 kb on + strand, within PS417_12180 at 2645.782 kb on + strand, within PS417_12180 at 2645.789 kb on - strand, within PS417_12180 at 2645.869 kb on + strand, within PS417_12180 at 2645.869 kb on + strand, within PS417_12180 at 2645.870 kb on - strand, within PS417_12180 at 2645.870 kb on - strand, within PS417_12180 at 2645.932 kb on + strand, within PS417_12180 at 2646.239 kb on - strand, within PS417_12180 at 2646.239 kb on - strand, within PS417_12180 at 2646.239 kb on - strand, within PS417_12180 at 2646.242 kb on + strand, within PS417_12180 at 2646.243 kb on - strand, within PS417_12180 at 2646.243 kb on - strand, within PS417_12180 at 2646.243 kb on - strand, within PS417_12180 at 2646.346 kb on + strand, within PS417_12180 at 2646.469 kb on + strand, within PS417_12180 at 2646.486 kb on + strand, within PS417_12180 at 2646.486 kb on + strand, within PS417_12180 at 2646.637 kb on + strand, within PS417_12180 at 2646.871 kb on + strand, within PS417_12180 at 2646.876 kb on + strand, within PS417_12180 at 2646.876 kb on + strand, within PS417_12180 at 2646.877 kb on - strand, within PS417_12180 at 2646.997 kb on + strand, within PS417_12180 at 2647.160 kb on - strand at 2647.216 kb on + strand at 2647.217 kb on - strand at 2647.327 kb on + strand at 2647.798 kb on + strand at 2647.799 kb on - strand at 2647.799 kb on - strand at 2647.802 kb on + strand at 2647.803 kb on - strand at 2647.803 kb on - strand at 2647.999 kb on - strand, within PS417_12190 at 2648.218 kb on + strand, within PS417_12190 at 2648.219 kb on - strand, within PS417_12190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,644,591 + PS417_12175 0.20 +0.4 2,644,648 + PS417_12175 0.25 -1.0 2,644,658 + PS417_12175 0.26 -1.7 2,644,659 - PS417_12175 0.26 +1.5 2,645,040 - PS417_12175 0.62 -0.3 2,645,154 - PS417_12175 0.73 +0.7 2,645,605 + -0.4 2,645,605 + +0.9 2,645,605 + +0.7 2,645,606 - -0.0 2,645,623 + +0.1 2,645,624 - -0.4 2,645,624 - +2.1 2,645,624 - +0.9 2,645,741 - PS417_12180 0.16 +0.7 2,645,741 - PS417_12180 0.16 +0.2 2,645,782 + PS417_12180 0.18 -2.3 2,645,782 + PS417_12180 0.18 -0.6 2,645,782 + PS417_12180 0.18 +1.5 2,645,789 - PS417_12180 0.19 +1.5 2,645,869 + PS417_12180 0.23 -1.9 2,645,869 + PS417_12180 0.23 +0.6 2,645,870 - PS417_12180 0.23 +1.8 2,645,870 - PS417_12180 0.23 +0.9 2,645,932 + PS417_12180 0.26 -0.2 2,646,239 - PS417_12180 0.43 +0.4 2,646,239 - PS417_12180 0.43 +1.7 2,646,239 - PS417_12180 0.43 +0.9 2,646,242 + PS417_12180 0.43 +1.3 2,646,243 - PS417_12180 0.43 +0.3 2,646,243 - PS417_12180 0.43 +0.3 2,646,243 - PS417_12180 0.43 +3.0 2,646,346 + PS417_12180 0.49 +0.7 2,646,469 + PS417_12180 0.55 -0.6 2,646,486 + PS417_12180 0.56 +1.7 2,646,486 + PS417_12180 0.56 -0.3 2,646,637 + PS417_12180 0.64 -0.2 2,646,871 + PS417_12180 0.77 +0.5 2,646,876 + PS417_12180 0.77 -0.4 2,646,876 + PS417_12180 0.77 +0.4 2,646,877 - PS417_12180 0.77 +0.1 2,646,997 + PS417_12180 0.84 +1.7 2,647,160 - +0.4 2,647,216 + +0.7 2,647,217 - +0.4 2,647,327 + +0.9 2,647,798 + -0.6 2,647,799 - +0.2 2,647,799 - -0.7 2,647,802 + -1.0 2,647,803 - -0.6 2,647,803 - -0.2 2,647,999 - PS417_12190 0.10 +1.2 2,648,218 + PS417_12190 0.24 -0.2 2,648,219 - PS417_12190 0.24 -1.7
Or see this region's nucleotide sequence