Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11850 and PS417_11855 are separated by 150 nucleotides PS417_11855 and PS417_11860 are separated by 3 nucleotides PS417_11860 and PS417_11865 are separated by 28 nucleotides
PS417_11850: PS417_11850 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 2,578,967 to 2,580,904
_11850
PS417_11855: PS417_11855 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 2,581,055 to 2,581,942
_11855
PS417_11860: PS417_11860 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 2,581,946 to 2,582,734
_11860
PS417_11865: PS417_11865 - xylose isomerase, at 2,582,763 to 2,583,584
_11865
Position (kb)
2581
2582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2580.467 kb on + strand, within PS417_11850 at 2580.468 kb on - strand at 2580.575 kb on + strand at 2580.954 kb on + strand at 2581.005 kb on + strand at 2581.005 kb on + strand at 2581.523 kb on - strand, within PS417_11855 at 2581.532 kb on + strand, within PS417_11855 at 2581.532 kb on + strand, within PS417_11855 at 2581.616 kb on + strand, within PS417_11855 at 2581.617 kb on - strand, within PS417_11855 at 2581.617 kb on - strand, within PS417_11855 at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.884 kb on - strand at 2581.884 kb on - strand at 2581.884 kb on - strand at 2581.884 kb on - strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.889 kb on + strand at 2581.890 kb on - strand at 2581.890 kb on - strand at 2582.015 kb on + strand at 2582.016 kb on - strand at 2582.242 kb on - strand, within PS417_11860 at 2582.406 kb on + strand, within PS417_11860 at 2582.407 kb on - strand, within PS417_11860 at 2582.411 kb on - strand, within PS417_11860 at 2582.496 kb on - strand, within PS417_11860 at 2582.651 kb on + strand, within PS417_11860 at 2582.652 kb on - strand, within PS417_11860 at 2582.652 kb on - strand at 2582.652 kb on - strand, within PS417_11860 at 2582.776 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,580,467 + PS417_11850 0.77 -0.6 2,580,468 - -1.7 2,580,575 + -0.6 2,580,954 + +1.7 2,581,005 + +1.4 2,581,005 + -0.7 2,581,523 - PS417_11855 0.53 -0.0 2,581,532 + PS417_11855 0.54 +1.9 2,581,532 + PS417_11855 0.54 +0.7 2,581,616 + PS417_11855 0.63 +1.0 2,581,617 - PS417_11855 0.63 -0.4 2,581,617 - PS417_11855 0.63 +1.5 2,581,883 + -0.5 2,581,883 + -1.0 2,581,883 + +0.9 2,581,883 + -0.2 2,581,883 + -3.5 2,581,884 - -1.0 2,581,884 - -1.0 2,581,884 - +2.5 2,581,884 - +1.7 2,581,885 + -3.3 2,581,885 + -0.1 2,581,885 + +0.8 2,581,885 + +0.6 2,581,885 + -0.2 2,581,885 + -0.6 2,581,885 + -0.4 2,581,886 - +0.5 2,581,886 - -0.1 2,581,886 - +0.9 2,581,886 - +1.5 2,581,886 - +0.4 2,581,886 - +1.7 2,581,886 - -0.5 2,581,889 + +1.0 2,581,890 - -1.7 2,581,890 - -0.8 2,582,015 + +0.7 2,582,016 - -1.5 2,582,242 - PS417_11860 0.38 -0.0 2,582,406 + PS417_11860 0.58 +0.3 2,582,407 - PS417_11860 0.58 -1.3 2,582,411 - PS417_11860 0.59 -0.2 2,582,496 - PS417_11860 0.70 -2.2 2,582,651 + PS417_11860 0.89 -0.1 2,582,652 - PS417_11860 0.89 +1.1 2,582,652 - +1.4 2,582,652 - PS417_11860 0.89 +1.7 2,582,776 + +1.2
Or see this region's nucleotide sequence