Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11295 and PS417_11300 are separated by 352 nucleotides PS417_11300 and PS417_11305 overlap by 1 nucleotides
PS417_11295: PS417_11295 - general secretion pathway protein GspD, at 2,416,814 to 2,419,108
_11295
PS417_11300: PS417_11300 - general secretion pathway protein GspE, at 2,419,461 to 2,420,879
_11300
PS417_11305: PS417_11305 - general secretion pathway protein GspF, at 2,420,879 to 2,422,090
_11305
Position (kb)
2419
2420
2421 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2418.739 kb on + strand, within PS417_11295 at 2418.739 kb on + strand, within PS417_11295 at 2418.911 kb on + strand at 2418.912 kb on - strand at 2418.943 kb on + strand at 2418.944 kb on - strand at 2418.967 kb on + strand at 2419.003 kb on + strand at 2419.121 kb on + strand at 2419.121 kb on + strand at 2419.122 kb on - strand at 2419.122 kb on - strand at 2419.122 kb on - strand at 2419.361 kb on + strand at 2419.436 kb on + strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.437 kb on - strand at 2419.468 kb on + strand at 2419.469 kb on - strand at 2419.531 kb on + strand at 2419.703 kb on - strand, within PS417_11300 at 2419.703 kb on - strand, within PS417_11300 at 2419.728 kb on - strand, within PS417_11300 at 2419.728 kb on - strand, within PS417_11300 at 2420.230 kb on - strand, within PS417_11300 at 2420.894 kb on + strand at 2421.269 kb on + strand, within PS417_11305 at 2421.339 kb on - strand, within PS417_11305 at 2421.443 kb on + strand, within PS417_11305 at 2421.444 kb on - strand, within PS417_11305 at 2421.444 kb on - strand, within PS417_11305 at 2421.444 kb on - strand, within PS417_11305 at 2421.597 kb on - strand, within PS417_11305
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,418,739 + PS417_11295 0.84 +0.1 2,418,739 + PS417_11295 0.84 -1.1 2,418,911 + +1.2 2,418,912 - +1.1 2,418,943 + +0.1 2,418,944 - +0.4 2,418,967 + -0.2 2,419,003 + +0.3 2,419,121 + -1.1 2,419,121 + -0.3 2,419,122 - -0.1 2,419,122 - +0.2 2,419,122 - -1.3 2,419,361 + +0.2 2,419,436 + -0.6 2,419,437 - -0.8 2,419,437 - +0.3 2,419,437 - +0.2 2,419,437 - +1.0 2,419,437 - +0.2 2,419,437 - -1.2 2,419,468 + -1.2 2,419,469 - -0.4 2,419,531 + -0.5 2,419,703 - PS417_11300 0.17 +0.1 2,419,703 - PS417_11300 0.17 -0.2 2,419,728 - PS417_11300 0.19 +3.3 2,419,728 - PS417_11300 0.19 -0.3 2,420,230 - PS417_11300 0.54 +0.2 2,420,894 + -1.7 2,421,269 + PS417_11305 0.32 -2.4 2,421,339 - PS417_11305 0.38 +0.5 2,421,443 + PS417_11305 0.47 -2.3 2,421,444 - PS417_11305 0.47 -2.0 2,421,444 - PS417_11305 0.47 -0.1 2,421,444 - PS417_11305 0.47 -0.0 2,421,597 - PS417_11305 0.59 +1.3
Or see this region's nucleotide sequence