Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09490 and PS417_09495 overlap by 4 nucleotides PS417_09495 and PS417_09500 are separated by 35 nucleotides PS417_09500 and PS417_09505 are separated by 111 nucleotides
PS417_09490: PS417_09490 - quinone oxidoreductase, at 2,055,269 to 2,056,255
_09490
PS417_09495: PS417_09495 - DSBA oxidoreductase, at 2,056,252 to 2,056,842
_09495
PS417_09500: PS417_09500 - glucose 1-dehydrogenase, at 2,056,878 to 2,057,609
_09500
PS417_09505: PS417_09505 - hypothetical protein, at 2,057,721 to 2,058,002
_09505
Position (kb)
2056
2057 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2055.530 kb on + strand at 2055.530 kb on + strand at 2055.531 kb on - strand, within PS417_09490 at 2055.531 kb on - strand, within PS417_09490 at 2055.531 kb on - strand, within PS417_09490 at 2055.531 kb on - strand, within PS417_09490 at 2055.776 kb on + strand, within PS417_09490 at 2055.791 kb on + strand, within PS417_09490 at 2055.791 kb on + strand, within PS417_09490 at 2055.792 kb on - strand, within PS417_09490 at 2055.792 kb on - strand, within PS417_09490 at 2055.921 kb on - strand, within PS417_09490 at 2055.921 kb on - strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.975 kb on - strand, within PS417_09490 at 2055.987 kb on + strand, within PS417_09490 at 2056.049 kb on + strand, within PS417_09490 at 2056.063 kb on + strand, within PS417_09490 at 2056.063 kb on + strand, within PS417_09490 at 2056.205 kb on + strand at 2056.313 kb on - strand, within PS417_09495 at 2056.384 kb on + strand, within PS417_09495 at 2056.384 kb on + strand, within PS417_09495 at 2056.573 kb on + strand at 2056.574 kb on - strand at 2056.574 kb on - strand, within PS417_09495 at 2056.622 kb on + strand, within PS417_09495 at 2056.622 kb on + strand, within PS417_09495 at 2056.659 kb on - strand, within PS417_09495 at 2056.736 kb on + strand, within PS417_09495 at 2056.736 kb on + strand, within PS417_09495 at 2056.836 kb on + strand at 2056.837 kb on - strand at 2056.860 kb on + strand at 2056.860 kb on + strand at 2056.949 kb on - strand at 2056.954 kb on + strand, within PS417_09500 at 2056.954 kb on + strand, within PS417_09500 at 2056.955 kb on - strand, within PS417_09500 at 2056.955 kb on - strand, within PS417_09500 at 2057.294 kb on - strand, within PS417_09500 at 2057.417 kb on + strand, within PS417_09500 at 2057.418 kb on - strand, within PS417_09500 at 2057.418 kb on - strand, within PS417_09500 at 2057.465 kb on + strand, within PS417_09500 at 2057.466 kb on - strand, within PS417_09500 at 2057.472 kb on + strand, within PS417_09500 at 2057.521 kb on + strand, within PS417_09500 at 2057.522 kb on - strand, within PS417_09500 at 2057.645 kb on + strand at 2057.722 kb on + strand at 2057.723 kb on - strand at 2057.726 kb on + strand at 2057.726 kb on + strand at 2057.727 kb on - strand at 2057.755 kb on + strand, within PS417_09505
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,055,530 + -1.3 2,055,530 + +1.7 2,055,531 - PS417_09490 0.27 -0.5 2,055,531 - PS417_09490 0.27 -0.4 2,055,531 - PS417_09490 0.27 -0.8 2,055,531 - PS417_09490 0.27 +0.6 2,055,776 + PS417_09490 0.51 +0.5 2,055,791 + PS417_09490 0.53 -0.8 2,055,791 + PS417_09490 0.53 +0.5 2,055,792 - PS417_09490 0.53 -0.7 2,055,792 - PS417_09490 0.53 +0.2 2,055,921 - PS417_09490 0.66 -3.3 2,055,921 - PS417_09490 0.66 -0.8 2,055,974 + PS417_09490 0.71 -0.1 2,055,974 + PS417_09490 0.71 -0.1 2,055,974 + PS417_09490 0.71 +0.4 2,055,974 + PS417_09490 0.71 -0.3 2,055,975 - PS417_09490 0.72 -0.7 2,055,987 + PS417_09490 0.73 +1.3 2,056,049 + PS417_09490 0.79 -1.1 2,056,063 + PS417_09490 0.80 +0.2 2,056,063 + PS417_09490 0.80 -1.2 2,056,205 + -0.5 2,056,313 - PS417_09495 0.10 +0.0 2,056,384 + PS417_09495 0.22 +0.9 2,056,384 + PS417_09495 0.22 -1.4 2,056,573 + +0.7 2,056,574 - +1.2 2,056,574 - PS417_09495 0.54 -1.4 2,056,622 + PS417_09495 0.63 -0.1 2,056,622 + PS417_09495 0.63 -1.0 2,056,659 - PS417_09495 0.69 +0.2 2,056,736 + PS417_09495 0.82 -1.4 2,056,736 + PS417_09495 0.82 -0.2 2,056,836 + +0.9 2,056,837 - +0.0 2,056,860 + +3.1 2,056,860 + -0.7 2,056,949 - +1.9 2,056,954 + PS417_09500 0.10 -0.1 2,056,954 + PS417_09500 0.10 +0.7 2,056,955 - PS417_09500 0.11 +0.2 2,056,955 - PS417_09500 0.11 -0.2 2,057,294 - PS417_09500 0.57 +0.2 2,057,417 + PS417_09500 0.74 +0.7 2,057,418 - PS417_09500 0.74 -0.6 2,057,418 - PS417_09500 0.74 +0.5 2,057,465 + PS417_09500 0.80 -2.7 2,057,466 - PS417_09500 0.80 +0.3 2,057,472 + PS417_09500 0.81 -0.6 2,057,521 + PS417_09500 0.88 +1.3 2,057,522 - PS417_09500 0.88 +2.1 2,057,645 + -1.8 2,057,722 + -0.3 2,057,723 - -2.3 2,057,726 + +0.4 2,057,726 + +0.0 2,057,727 - +0.6 2,057,755 + PS417_09505 0.12 -0.1
Or see this region's nucleotide sequence