Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09480 and PS417_09485 overlap by 8 nucleotides PS417_09485 and PS417_09490 are separated by 115 nucleotides
PS417_09480: PS417_09480 - peptidase M24, at 2,052,444 to 2,054,252
_09480
PS417_09485: PS417_09485 - LysR family transcriptional regulator, at 2,054,245 to 2,055,153
_09485
PS417_09490: PS417_09490 - quinone oxidoreductase, at 2,055,269 to 2,056,255
_09490
Position (kb)
2054
2055
2056 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2053.476 kb on - strand, within PS417_09480 at 2053.537 kb on + strand, within PS417_09480 at 2053.537 kb on + strand, within PS417_09480 at 2053.538 kb on - strand, within PS417_09480 at 2053.538 kb on - strand, within PS417_09480 at 2053.538 kb on - strand, within PS417_09480 at 2053.566 kb on + strand, within PS417_09480 at 2053.593 kb on + strand, within PS417_09480 at 2053.854 kb on + strand, within PS417_09480 at 2053.855 kb on - strand, within PS417_09480 at 2053.879 kb on + strand, within PS417_09480 at 2054.292 kb on + strand at 2054.292 kb on + strand at 2054.293 kb on - strand at 2054.294 kb on + strand at 2054.294 kb on + strand at 2054.295 kb on - strand at 2054.295 kb on - strand at 2054.375 kb on + strand, within PS417_09485 at 2054.420 kb on + strand, within PS417_09485 at 2054.431 kb on + strand, within PS417_09485 at 2054.432 kb on - strand, within PS417_09485 at 2054.546 kb on + strand, within PS417_09485 at 2054.802 kb on - strand, within PS417_09485 at 2054.957 kb on + strand, within PS417_09485 at 2054.957 kb on + strand, within PS417_09485 at 2054.957 kb on + strand, within PS417_09485 at 2054.960 kb on + strand, within PS417_09485 at 2054.961 kb on - strand, within PS417_09485 at 2054.961 kb on - strand, within PS417_09485 at 2055.046 kb on + strand, within PS417_09485 at 2055.189 kb on + strand at 2055.191 kb on + strand at 2055.249 kb on + strand at 2055.249 kb on + strand at 2055.249 kb on + strand at 2055.249 kb on + strand at 2055.250 kb on - strand at 2055.250 kb on - strand at 2055.530 kb on + strand at 2055.530 kb on + strand at 2055.531 kb on - strand, within PS417_09490 at 2055.531 kb on - strand, within PS417_09490 at 2055.531 kb on - strand, within PS417_09490 at 2055.531 kb on - strand, within PS417_09490 at 2055.776 kb on + strand, within PS417_09490 at 2055.791 kb on + strand, within PS417_09490 at 2055.791 kb on + strand, within PS417_09490 at 2055.792 kb on - strand, within PS417_09490 at 2055.792 kb on - strand, within PS417_09490 at 2055.921 kb on - strand, within PS417_09490 at 2055.921 kb on - strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.974 kb on + strand, within PS417_09490 at 2055.975 kb on - strand, within PS417_09490 at 2055.987 kb on + strand, within PS417_09490 at 2056.049 kb on + strand, within PS417_09490 at 2056.063 kb on + strand, within PS417_09490 at 2056.063 kb on + strand, within PS417_09490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,053,476 - PS417_09480 0.57 -0.2 2,053,537 + PS417_09480 0.60 -2.4 2,053,537 + PS417_09480 0.60 -2.3 2,053,538 - PS417_09480 0.60 -1.1 2,053,538 - PS417_09480 0.60 -0.4 2,053,538 - PS417_09480 0.60 -0.6 2,053,566 + PS417_09480 0.62 +0.6 2,053,593 + PS417_09480 0.64 -0.9 2,053,854 + PS417_09480 0.78 -0.2 2,053,855 - PS417_09480 0.78 +0.5 2,053,879 + PS417_09480 0.79 +1.1 2,054,292 + +0.7 2,054,292 + -0.5 2,054,293 - -0.3 2,054,294 + -0.2 2,054,294 + -0.4 2,054,295 - -0.0 2,054,295 - -0.1 2,054,375 + PS417_09485 0.14 -0.4 2,054,420 + PS417_09485 0.19 +0.0 2,054,431 + PS417_09485 0.20 -0.7 2,054,432 - PS417_09485 0.21 +0.2 2,054,546 + PS417_09485 0.33 +0.2 2,054,802 - PS417_09485 0.61 -1.5 2,054,957 + PS417_09485 0.78 -0.4 2,054,957 + PS417_09485 0.78 -2.6 2,054,957 + PS417_09485 0.78 +0.3 2,054,960 + PS417_09485 0.79 -0.4 2,054,961 - PS417_09485 0.79 +0.1 2,054,961 - PS417_09485 0.79 -0.3 2,055,046 + PS417_09485 0.88 -1.3 2,055,189 + +0.7 2,055,191 + +0.6 2,055,249 + -0.8 2,055,249 + -0.1 2,055,249 + +1.1 2,055,249 + -0.6 2,055,250 - -0.7 2,055,250 - +1.9 2,055,530 + -1.3 2,055,530 + +1.7 2,055,531 - PS417_09490 0.27 -0.5 2,055,531 - PS417_09490 0.27 -0.4 2,055,531 - PS417_09490 0.27 -0.8 2,055,531 - PS417_09490 0.27 +0.6 2,055,776 + PS417_09490 0.51 +0.5 2,055,791 + PS417_09490 0.53 -0.8 2,055,791 + PS417_09490 0.53 +0.5 2,055,792 - PS417_09490 0.53 -0.7 2,055,792 - PS417_09490 0.53 +0.2 2,055,921 - PS417_09490 0.66 -3.3 2,055,921 - PS417_09490 0.66 -0.8 2,055,974 + PS417_09490 0.71 -0.1 2,055,974 + PS417_09490 0.71 -0.1 2,055,974 + PS417_09490 0.71 +0.4 2,055,974 + PS417_09490 0.71 -0.3 2,055,975 - PS417_09490 0.72 -0.7 2,055,987 + PS417_09490 0.73 +1.3 2,056,049 + PS417_09490 0.79 -1.1 2,056,063 + PS417_09490 0.80 +0.2 2,056,063 + PS417_09490 0.80 -1.2
Or see this region's nucleotide sequence