Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09215 and PS417_09220 are separated by 43 nucleotides PS417_09220 and PS417_09225 are separated by 108 nucleotides PS417_09225 and PS417_09230 are separated by 67 nucleotides
PS417_09215: PS417_09215 - MFS transporter, at 2,001,929 to 2,003,122
_09215
PS417_09220: PS417_09220 - cupin, at 2,003,166 to 2,003,477
_09220
PS417_09225: PS417_09225 - C4-dicarboxylate ABC transporter, at 2,003,586 to 2,004,734
_09225
PS417_09230: PS417_09230 - glycerol acyltransferase, at 2,004,802 to 2,006,676
_09230
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2002.605 kb on - strand, within PS417_09215 at 2002.940 kb on + strand, within PS417_09215 at 2002.940 kb on + strand, within PS417_09215 at 2002.941 kb on - strand, within PS417_09215 at 2002.941 kb on - strand at 2003.128 kb on - strand at 2003.128 kb on - strand at 2003.131 kb on - strand at 2003.394 kb on + strand, within PS417_09220 at 2003.394 kb on + strand, within PS417_09220 at 2003.546 kb on - strand at 2003.808 kb on + strand, within PS417_09225 at 2004.187 kb on - strand, within PS417_09225 at 2004.234 kb on - strand, within PS417_09225 at 2004.260 kb on + strand, within PS417_09225 at 2004.333 kb on - strand, within PS417_09225 at 2004.333 kb on - strand, within PS417_09225 at 2004.333 kb on - strand, within PS417_09225 at 2004.553 kb on - strand, within PS417_09225 at 2004.681 kb on + strand at 2004.682 kb on - strand at 2004.684 kb on - strand at 2004.732 kb on + strand at 2004.733 kb on - strand at 2004.742 kb on + strand at 2004.742 kb on + strand at 2004.743 kb on - strand at 2004.925 kb on + strand at 2004.925 kb on + strand at 2004.926 kb on - strand at 2004.955 kb on - strand at 2005.189 kb on + strand, within PS417_09230 at 2005.189 kb on + strand at 2005.576 kb on + strand at 2005.576 kb on + strand, within PS417_09230 at 2005.673 kb on + strand, within PS417_09230 at 2005.673 kb on + strand, within PS417_09230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,002,605 - PS417_09215 0.57 +0.2 2,002,940 + PS417_09215 0.85 +0.6 2,002,940 + PS417_09215 0.85 -0.4 2,002,941 - PS417_09215 0.85 -0.2 2,002,941 - +2.7 2,003,128 - +0.5 2,003,128 - -0.1 2,003,131 - -1.1 2,003,394 + PS417_09220 0.73 +0.5 2,003,394 + PS417_09220 0.73 -0.3 2,003,546 - +0.1 2,003,808 + PS417_09225 0.19 -1.7 2,004,187 - PS417_09225 0.52 -0.7 2,004,234 - PS417_09225 0.56 +0.1 2,004,260 + PS417_09225 0.59 -1.5 2,004,333 - PS417_09225 0.65 -2.1 2,004,333 - PS417_09225 0.65 -2.0 2,004,333 - PS417_09225 0.65 +0.2 2,004,553 - PS417_09225 0.84 -0.1 2,004,681 + +0.7 2,004,682 - -1.0 2,004,684 - -0.3 2,004,732 + +0.8 2,004,733 - -1.4 2,004,742 + -0.6 2,004,742 + +1.0 2,004,743 - -0.1 2,004,925 + -0.8 2,004,925 + +2.2 2,004,926 - -0.6 2,004,955 - -1.2 2,005,189 + PS417_09230 0.21 -1.1 2,005,189 + +0.7 2,005,576 + -0.0 2,005,576 + PS417_09230 0.41 -1.5 2,005,673 + PS417_09230 0.46 -0.8 2,005,673 + PS417_09230 0.46 +0.0
Or see this region's nucleotide sequence