Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07120 and PS417_07125 are separated by 32 nucleotides PS417_07125 and PS417_07130 are separated by 15 nucleotides PS417_07130 and PS417_07135 are separated by 234 nucleotides
PS417_07120: PS417_07120 - RNA polymerase sigma-H factor, at 1,575,007 to 1,575,588
_07120
PS417_07125: PS417_07125 - RNA polymerase sigma-H factor AlgU, at 1,575,621 to 1,576,208
_07125
PS417_07130: PS417_07130 - RNA polymerase sigma-H factor AlgU, at 1,576,224 to 1,577,186
_07130
PS417_07135: PS417_07135 - serine peptidase, at 1,577,421 to 1,578,860
_07135
Position (kb)
1576
1577
1578 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1575.230 kb on + strand, within PS417_07120 at 1575.263 kb on - strand, within PS417_07120 at 1575.263 kb on - strand, within PS417_07120 at 1575.302 kb on + strand, within PS417_07120 at 1575.303 kb on - strand, within PS417_07120 at 1575.303 kb on - strand, within PS417_07120 at 1575.475 kb on + strand, within PS417_07120 at 1575.475 kb on + strand, within PS417_07120 at 1576.224 kb on - strand at 1576.349 kb on + strand, within PS417_07130 at 1576.350 kb on - strand, within PS417_07130 at 1576.360 kb on - strand, within PS417_07130 at 1576.575 kb on + strand, within PS417_07130 at 1576.859 kb on + strand, within PS417_07130 at 1576.962 kb on + strand, within PS417_07130 at 1576.962 kb on + strand, within PS417_07130 at 1576.962 kb on + strand, within PS417_07130 at 1576.963 kb on - strand, within PS417_07130 at 1576.963 kb on - strand, within PS417_07130 at 1576.963 kb on - strand, within PS417_07130 at 1577.060 kb on - strand, within PS417_07130 at 1577.075 kb on + strand, within PS417_07130 at 1577.198 kb on + strand at 1577.198 kb on + strand at 1577.199 kb on - strand at 1577.199 kb on - strand at 1577.199 kb on - strand at 1577.222 kb on + strand at 1577.223 kb on - strand at 1577.223 kb on - strand at 1577.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,575,230 + PS417_07120 0.38 -0.9 1,575,263 - PS417_07120 0.44 -1.3 1,575,263 - PS417_07120 0.44 +0.4 1,575,302 + PS417_07120 0.51 -2.3 1,575,303 - PS417_07120 0.51 +0.2 1,575,303 - PS417_07120 0.51 -2.1 1,575,475 + PS417_07120 0.80 +1.1 1,575,475 + PS417_07120 0.80 +0.3 1,576,224 - -0.6 1,576,349 + PS417_07130 0.13 +0.9 1,576,350 - PS417_07130 0.13 -2.6 1,576,360 - PS417_07130 0.14 +0.4 1,576,575 + PS417_07130 0.36 -3.0 1,576,859 + PS417_07130 0.66 -0.8 1,576,962 + PS417_07130 0.77 -1.0 1,576,962 + PS417_07130 0.77 -0.2 1,576,962 + PS417_07130 0.77 -0.3 1,576,963 - PS417_07130 0.77 -0.5 1,576,963 - PS417_07130 0.77 +0.2 1,576,963 - PS417_07130 0.77 +0.1 1,577,060 - PS417_07130 0.87 -2.8 1,577,075 + PS417_07130 0.88 -1.6 1,577,198 + -0.1 1,577,198 + -0.0 1,577,199 - +0.1 1,577,199 - -1.1 1,577,199 - -0.9 1,577,222 + +0.5 1,577,223 - -1.5 1,577,223 - -1.3 1,577,223 - -0.1
Or see this region's nucleotide sequence