Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04515 and PS417_04520 are separated by 134 nucleotides PS417_04520 and PS417_04525 are separated by 260 nucleotides PS417_04525 and PS417_04530 are separated by 30 nucleotides
PS417_04515: PS417_04515 - peptidase M48, at 997,720 to 998,538
_04515
PS417_04520: PS417_04520 - membrane protein, at 998,673 to 999,257
_04520
PS417_04525: PS417_04525 - 16S rRNA methyltransferase, at 999,518 to 1,000,516
_04525
PS417_04530: PS417_04530 - glycerate dehydrogenase, at 1,000,547 to 1,001,512
_04530
Position (kb) 999
1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 998.543 kb on - strand at 998.562 kb on - strand at 998.608 kb on - strand at 998.608 kb on - strand at 998.674 kb on + strand at 998.674 kb on + strand at 998.675 kb on - strand at 998.675 kb on - strand at 998.704 kb on + strand at 998.705 kb on - strand at 998.705 kb on - strand at 998.705 kb on - strand at 999.262 kb on + strand at 999.263 kb on - strand at 999.335 kb on + strand at 999.335 kb on + strand at 999.336 kb on - strand at 999.405 kb on + strand at 999.405 kb on + strand at 999.405 kb on + strand at 999.405 kb on + strand at 999.406 kb on - strand at 999.406 kb on - strand at 999.406 kb on - strand at 999.406 kb on - strand at 999.447 kb on + strand at 999.448 kb on - strand at 999.735 kb on - strand, within PS417_04525 at 999.854 kb on + strand, within PS417_04525 at 999.854 kb on + strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 999.855 kb on - strand, within PS417_04525 at 1000.371 kb on - strand, within PS417_04525 at 1000.496 kb on + strand at 1000.497 kb on - strand at 1000.497 kb on - strand at 1000.715 kb on + strand, within PS417_04530 at 1000.715 kb on + strand, within PS417_04530 at 1000.715 kb on + strand, within PS417_04530 at 1000.724 kb on + strand, within PS417_04530 at 1001.140 kb on - strand, within PS417_04530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 998,543 - +0.7 998,562 - +0.3 998,608 - +0.8 998,608 - -3.0 998,674 + +0.8 998,674 + +2.4 998,675 - -1.0 998,675 - +0.0 998,704 + +0.9 998,705 - -1.1 998,705 - -0.2 998,705 - -0.2 999,262 + +0.1 999,263 - +3.1 999,335 + -0.9 999,335 + -2.5 999,336 - -0.6 999,405 + -1.3 999,405 + -0.9 999,405 + +0.0 999,405 + +2.6 999,406 - +0.6 999,406 - +0.1 999,406 - +1.1 999,406 - -0.2 999,447 + +0.2 999,448 - -0.2 999,735 - PS417_04525 0.22 -1.4 999,854 + PS417_04525 0.34 +0.9 999,854 + PS417_04525 0.34 -1.9 999,855 - PS417_04525 0.34 -3.1 999,855 - PS417_04525 0.34 -0.6 999,855 - PS417_04525 0.34 +0.2 999,855 - PS417_04525 0.34 -1.5 999,855 - PS417_04525 0.34 -1.1 1,000,371 - PS417_04525 0.85 -1.2 1,000,496 + -0.0 1,000,497 - -0.4 1,000,497 - -1.3 1,000,715 + PS417_04530 0.17 +1.6 1,000,715 + PS417_04530 0.17 -0.5 1,000,715 + PS417_04530 0.17 +4.7 1,000,724 + PS417_04530 0.18 -2.5 1,001,140 - PS417_04530 0.61 -0.6
Or see this region's nucleotide sequence