Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04295 and PS417_04300 are separated by 150 nucleotides PS417_04300 and PS417_04305 overlap by 8 nucleotides PS417_04305 and PS417_04310 are separated by 27 nucleotides PS417_04310 and PS417_04315 are separated by 1 nucleotides
PS417_04295: PS417_04295 - peptidase PmbA, at 963,706 to 965,052
_04295
PS417_04300: PS417_04300 - FagA protein, at 965,203 to 965,598
_04300
PS417_04305: PS417_04305 - aspartate ammonia-lyase, at 965,591 to 966,967
_04305
PS417_04310: PS417_04310 - hypothetical protein, at 966,995 to 967,423
_04310
PS417_04315: PS417_04315 - superoxide dismutase, at 967,425 to 968,036
_04315
Position (kb) 965
966
967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 964.608 kb on + strand, within PS417_04295 at 964.678 kb on + strand, within PS417_04295 at 964.678 kb on + strand, within PS417_04295 at 964.679 kb on - strand, within PS417_04295 at 964.679 kb on - strand, within PS417_04295 at 964.679 kb on - strand, within PS417_04295 at 964.679 kb on - strand, within PS417_04295 at 964.693 kb on + strand, within PS417_04295 at 964.693 kb on + strand, within PS417_04295 at 964.694 kb on - strand, within PS417_04295 at 964.694 kb on - strand, within PS417_04295 at 964.694 kb on - strand, within PS417_04295 at 964.861 kb on + strand, within PS417_04295 at 964.862 kb on - strand, within PS417_04295 at 965.074 kb on + strand at 965.308 kb on + strand, within PS417_04300 at 965.309 kb on - strand, within PS417_04300 at 965.309 kb on - strand, within PS417_04300 at 965.309 kb on - strand, within PS417_04300 at 965.596 kb on + strand at 965.596 kb on + strand at 965.596 kb on + strand at 965.597 kb on - strand at 965.654 kb on + strand at 965.654 kb on + strand at 965.654 kb on + strand at 965.654 kb on + strand at 965.655 kb on - strand at 965.655 kb on - strand at 965.655 kb on - strand at 965.891 kb on - strand, within PS417_04305 at 966.225 kb on + strand, within PS417_04305 at 966.965 kb on + strand at 967.039 kb on + strand, within PS417_04310 at 967.039 kb on + strand, within PS417_04310 at 967.040 kb on - strand, within PS417_04310 at 967.073 kb on + strand, within PS417_04310 at 967.073 kb on + strand, within PS417_04310 at 967.074 kb on - strand, within PS417_04310 at 967.424 kb on + strand at 967.425 kb on - strand at 967.431 kb on + strand at 967.431 kb on + strand at 967.432 kb on - strand at 967.432 kb on - strand at 967.432 kb on - strand at 967.433 kb on + strand at 967.433 kb on + strand at 967.458 kb on + strand at 967.478 kb on + strand at 967.507 kb on - strand, within PS417_04315 at 967.507 kb on - strand, within PS417_04315 at 967.507 kb on - strand, within PS417_04315 at 967.527 kb on + strand, within PS417_04315 at 967.527 kb on + strand, within PS417_04315 at 967.935 kb on + strand, within PS417_04315 at 967.936 kb on - strand, within PS417_04315 at 967.936 kb on - strand, within PS417_04315 at 967.945 kb on - strand, within PS417_04315 at 967.966 kb on - strand, within PS417_04315
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 964,608 + PS417_04295 0.67 +0.0 964,678 + PS417_04295 0.72 -0.8 964,678 + PS417_04295 0.72 -1.9 964,679 - PS417_04295 0.72 -0.2 964,679 - PS417_04295 0.72 -0.9 964,679 - PS417_04295 0.72 +0.4 964,679 - PS417_04295 0.72 +2.1 964,693 + PS417_04295 0.73 -0.1 964,693 + PS417_04295 0.73 +0.1 964,694 - PS417_04295 0.73 -0.8 964,694 - PS417_04295 0.73 -1.2 964,694 - PS417_04295 0.73 +0.3 964,861 + PS417_04295 0.86 +1.9 964,862 - PS417_04295 0.86 -1.9 965,074 + +2.5 965,308 + PS417_04300 0.27 -3.3 965,309 - PS417_04300 0.27 +0.7 965,309 - PS417_04300 0.27 -0.4 965,309 - PS417_04300 0.27 +0.8 965,596 + -0.0 965,596 + +2.3 965,596 + +1.0 965,597 - +0.5 965,654 + -0.9 965,654 + +0.6 965,654 + -0.9 965,654 + +2.2 965,655 - -0.2 965,655 - -1.3 965,655 - +1.0 965,891 - PS417_04305 0.22 -0.9 966,225 + PS417_04305 0.46 -0.9 966,965 + +0.7 967,039 + PS417_04310 0.10 +0.4 967,039 + PS417_04310 0.10 +1.7 967,040 - PS417_04310 0.10 +0.2 967,073 + PS417_04310 0.18 -0.9 967,073 + PS417_04310 0.18 +3.9 967,074 - PS417_04310 0.18 -0.4 967,424 + +0.6 967,425 - -0.4 967,431 + +1.1 967,431 + +0.1 967,432 - -1.7 967,432 - +0.1 967,432 - -0.6 967,433 + -0.7 967,433 + -1.1 967,458 + -0.0 967,478 + -0.1 967,507 - PS417_04315 0.13 -0.4 967,507 - PS417_04315 0.13 -0.0 967,507 - PS417_04315 0.13 -0.3 967,527 + PS417_04315 0.17 +1.1 967,527 + PS417_04315 0.17 -0.8 967,935 + PS417_04315 0.83 -0.2 967,936 - PS417_04315 0.83 -1.6 967,936 - PS417_04315 0.83 -1.5 967,945 - PS417_04315 0.85 -2.1 967,966 - PS417_04315 0.88 -1.5
Or see this region's nucleotide sequence