Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04215 and PS417_04220 overlap by 4 nucleotides PS417_04220 and PS417_04225 overlap by 4 nucleotides PS417_04225 and PS417_04230 are separated by 58 nucleotides PS417_04230 and PS417_04235 are separated by 157 nucleotides
PS417_04215: PS417_04215 - malate transporter, at 945,469 to 946,410
_04215
PS417_04220: PS417_04220 - 4-carboxymuconolactone decarboxylase, at 946,407 to 946,787
_04220
PS417_04225: PS417_04225 - conjugal transfer protein TraR, at 946,784 to 947,830
_04225
PS417_04230: PS417_04230 - lipoprotein, at 947,889 to 948,260
_04230
PS417_04235: PS417_04235 - glutamyl-tRNA amidotransferase, at 948,418 to 949,863
_04235
Position (kb) 946
947
948 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 945.829 kb on + strand, within PS417_04215 at 945.886 kb on + strand, within PS417_04215 at 945.887 kb on - strand, within PS417_04215 at 945.920 kb on - strand, within PS417_04215 at 945.920 kb on - strand, within PS417_04215 at 946.252 kb on + strand, within PS417_04215 at 946.252 kb on + strand, within PS417_04215 at 946.253 kb on - strand, within PS417_04215 at 946.408 kb on + strand at 946.409 kb on - strand at 946.484 kb on - strand, within PS417_04220 at 946.866 kb on + strand at 946.866 kb on + strand at 946.866 kb on + strand at 946.866 kb on + strand at 946.866 kb on + strand at 946.867 kb on - strand at 946.869 kb on + strand at 946.869 kb on + strand at 946.869 kb on + strand at 946.869 kb on + strand at 946.870 kb on - strand at 946.870 kb on - strand at 946.870 kb on - strand at 946.870 kb on - strand at 946.915 kb on + strand, within PS417_04225 at 946.915 kb on + strand, within PS417_04225 at 947.620 kb on + strand, within PS417_04225 at 948.131 kb on + strand, within PS417_04230 at 948.132 kb on - strand, within PS417_04230 at 948.132 kb on - strand, within PS417_04230 at 948.157 kb on + strand, within PS417_04230 at 948.157 kb on + strand, within PS417_04230 at 948.158 kb on - strand, within PS417_04230 at 948.158 kb on - strand, within PS417_04230 at 948.312 kb on + strand at 948.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 945,829 + PS417_04215 0.38 +1.7 945,886 + PS417_04215 0.44 +0.3 945,887 - PS417_04215 0.44 -0.3 945,920 - PS417_04215 0.48 +0.4 945,920 - PS417_04215 0.48 +0.4 946,252 + PS417_04215 0.83 +0.2 946,252 + PS417_04215 0.83 -0.1 946,253 - PS417_04215 0.83 +0.9 946,408 + -2.6 946,409 - -0.3 946,484 - PS417_04220 0.20 +0.1 946,866 + -0.3 946,866 + -0.1 946,866 + -0.3 946,866 + +0.5 946,866 + -0.7 946,867 - +3.1 946,869 + -0.2 946,869 + -2.2 946,869 + -0.4 946,869 + -1.1 946,870 - +0.7 946,870 - -1.1 946,870 - +0.4 946,870 - -0.1 946,915 + PS417_04225 0.13 -1.0 946,915 + PS417_04225 0.13 -0.4 947,620 + PS417_04225 0.80 -1.7 948,131 + PS417_04230 0.65 -0.1 948,132 - PS417_04230 0.65 -0.6 948,132 - PS417_04230 0.65 +0.6 948,157 + PS417_04230 0.72 -1.4 948,157 + PS417_04230 0.72 -1.1 948,158 - PS417_04230 0.72 -0.6 948,158 - PS417_04230 0.72 +0.1 948,312 + -0.2 948,313 - +2.5
Or see this region's nucleotide sequence