Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04190 and PS417_04195 are separated by 234 nucleotides PS417_04195 and PS417_04200 are separated by 54 nucleotides
PS417_04190: PS417_04190 - phosphatidylcholine-hydrolyzing phospholipase, at 938,447 to 939,604
_04190
PS417_04195: PS417_04195 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) (from data) , at 939,839 to 940,750
_04195
PS417_04200: PS417_04200 - 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (from data) , at 940,805 to 942,250
_04200
Position (kb)
939
940
941 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 938.912 kb on + strand, within PS417_04190 at 938.977 kb on - strand, within PS417_04190 at 939.057 kb on + strand, within PS417_04190 at 939.187 kb on + strand, within PS417_04190 at 939.187 kb on + strand, within PS417_04190 at 939.187 kb on + strand, within PS417_04190 at 939.187 kb on + strand, within PS417_04190 at 939.188 kb on - strand, within PS417_04190 at 939.322 kb on + strand, within PS417_04190 at 939.323 kb on - strand, within PS417_04190 at 939.323 kb on - strand, within PS417_04190 at 939.348 kb on - strand, within PS417_04190 at 939.348 kb on - strand, within PS417_04190 at 939.400 kb on - strand, within PS417_04190 at 939.400 kb on - strand, within PS417_04190 at 939.400 kb on - strand, within PS417_04190 at 939.433 kb on - strand, within PS417_04190 at 939.433 kb on - strand, within PS417_04190 at 939.607 kb on + strand at 939.607 kb on + strand at 939.608 kb on - strand at 939.608 kb on - strand at 939.608 kb on - strand at 939.618 kb on + strand at 939.618 kb on + strand at 939.618 kb on + strand at 939.618 kb on + strand at 939.618 kb on + strand at 939.618 kb on + strand at 939.618 kb on + strand at 939.619 kb on - strand at 939.619 kb on - strand at 939.619 kb on - strand at 939.619 kb on - strand at 939.619 kb on - strand at 939.672 kb on + strand at 939.673 kb on - strand at 939.673 kb on - strand at 939.837 kb on + strand at 939.838 kb on - strand at 939.936 kb on - strand, within PS417_04195 at 940.179 kb on - strand, within PS417_04195 at 940.179 kb on - strand, within PS417_04195 at 940.374 kb on + strand, within PS417_04195 at 940.404 kb on - strand, within PS417_04195 at 940.404 kb on - strand, within PS417_04195 at 940.470 kb on - strand, within PS417_04195 at 940.517 kb on + strand, within PS417_04195 at 940.707 kb on - strand at 940.758 kb on - strand at 940.758 kb on - strand at 940.758 kb on - strand at 940.758 kb on - strand at 940.758 kb on - strand at 940.758 kb on - strand at 940.832 kb on + strand at 940.832 kb on + strand at 940.832 kb on + strand at 940.832 kb on + strand at 940.833 kb on - strand at 940.833 kb on - strand at 940.833 kb on - strand at 940.833 kb on - strand at 941.131 kb on + strand, within PS417_04200 at 941.178 kb on - strand, within PS417_04200 at 941.178 kb on - strand, within PS417_04200 at 941.208 kb on + strand, within PS417_04200 at 941.208 kb on + strand, within PS417_04200 at 941.209 kb on - strand, within PS417_04200 at 941.209 kb on - strand, within PS417_04200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 938,912 + PS417_04190 0.40 -0.6 938,977 - PS417_04190 0.46 +1.1 939,057 + PS417_04190 0.53 -0.5 939,187 + PS417_04190 0.64 -0.5 939,187 + PS417_04190 0.64 +0.2 939,187 + PS417_04190 0.64 +0.8 939,187 + PS417_04190 0.64 +0.8 939,188 - PS417_04190 0.64 -1.7 939,322 + PS417_04190 0.76 -0.2 939,323 - PS417_04190 0.76 +0.1 939,323 - PS417_04190 0.76 -0.9 939,348 - PS417_04190 0.78 +0.4 939,348 - PS417_04190 0.78 +0.4 939,400 - PS417_04190 0.82 -1.8 939,400 - PS417_04190 0.82 -2.0 939,400 - PS417_04190 0.82 -0.9 939,433 - PS417_04190 0.85 -0.8 939,433 - PS417_04190 0.85 +0.9 939,607 + -0.2 939,607 + -1.2 939,608 - +0.1 939,608 - -1.7 939,608 - +0.0 939,618 + +2.5 939,618 + -0.7 939,618 + +0.9 939,618 + -0.4 939,618 + -2.0 939,618 + +0.4 939,618 + -1.2 939,619 - -0.4 939,619 - -0.9 939,619 - +0.4 939,619 - -2.3 939,619 - +1.3 939,672 + +1.4 939,673 - +0.6 939,673 - -0.7 939,837 + -0.5 939,838 - +0.0 939,936 - PS417_04195 0.11 +0.0 940,179 - PS417_04195 0.37 -0.5 940,179 - PS417_04195 0.37 -0.8 940,374 + PS417_04195 0.59 +0.1 940,404 - PS417_04195 0.62 -0.9 940,404 - PS417_04195 0.62 +0.1 940,470 - PS417_04195 0.69 +1.9 940,517 + PS417_04195 0.74 -1.1 940,707 - +0.9 940,758 - -0.6 940,758 - -0.2 940,758 - +0.4 940,758 - -0.8 940,758 - -1.1 940,758 - +0.5 940,832 + -0.4 940,832 + +0.4 940,832 + -0.9 940,832 + -0.8 940,833 - -1.2 940,833 - +0.6 940,833 - -1.3 940,833 - +0.1 941,131 + PS417_04200 0.23 -0.8 941,178 - PS417_04200 0.26 -0.4 941,178 - PS417_04200 0.26 -2.7 941,208 + PS417_04200 0.28 -0.1 941,208 + PS417_04200 0.28 +1.3 941,209 - PS417_04200 0.28 +0.8 941,209 - PS417_04200 0.28 +0.3
Or see this region's nucleotide sequence