Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03390 and PS417_03395 are separated by 141 nucleotides PS417_03395 and PS417_03400 are separated by 44 nucleotides PS417_03400 and PS417_03405 overlap by 11 nucleotides PS417_03405 and PS417_03410 are separated by 25 nucleotides
PS417_03390: PS417_03390 - AraC family transcriptional regulator, at 778,338 to 779,159
_03390
PS417_03395: PS417_03395 - lysine transporter LysE, at 779,301 to 779,939
_03395
PS417_03400: PS417_03400 - D-alanine--D-alanine ligase, at 779,984 to 781,078
_03400
PS417_03405: PS417_03405 - D-alanyl-alanine synthetase, at 781,068 to 781,793
_03405
PS417_03410: PS417_03410 - gamma-glutamyltransferase, at 781,819 to 783,630
_03410
Position (kb) 779
780
781
782 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 779.041 kb on + strand, within PS417_03390 at 779.041 kb on + strand, within PS417_03390 at 779.041 kb on + strand, within PS417_03390 at 779.042 kb on - strand, within PS417_03390 at 779.042 kb on - strand, within PS417_03390 at 779.042 kb on - strand, within PS417_03390 at 779.189 kb on + strand at 779.189 kb on + strand at 779.190 kb on - strand at 779.190 kb on - strand at 779.231 kb on + strand at 779.259 kb on + strand at 779.259 kb on + strand at 779.514 kb on - strand, within PS417_03395 at 779.532 kb on + strand, within PS417_03395 at 779.532 kb on + strand, within PS417_03395 at 779.533 kb on - strand, within PS417_03395 at 779.533 kb on - strand, within PS417_03395 at 779.562 kb on + strand, within PS417_03395 at 779.562 kb on + strand, within PS417_03395 at 779.563 kb on - strand, within PS417_03395 at 779.571 kb on + strand, within PS417_03395 at 779.587 kb on + strand, within PS417_03395 at 779.587 kb on + strand, within PS417_03395 at 779.902 kb on - strand at 779.960 kb on + strand at 779.961 kb on - strand at 779.961 kb on - strand at 780.000 kb on + strand at 780.007 kb on + strand at 780.472 kb on + strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.488 kb on - strand, within PS417_03400 at 780.594 kb on - strand, within PS417_03400 at 780.743 kb on + strand, within PS417_03400 at 780.762 kb on - strand, within PS417_03400 at 780.966 kb on - strand, within PS417_03400 at 780.999 kb on - strand at 781.096 kb on + strand at 781.168 kb on + strand, within PS417_03405 at 781.168 kb on + strand, within PS417_03405 at 781.168 kb on + strand, within PS417_03405 at 781.405 kb on + strand, within PS417_03405 at 781.405 kb on + strand, within PS417_03405 at 781.406 kb on - strand, within PS417_03405 at 781.406 kb on - strand, within PS417_03405 at 781.494 kb on + strand, within PS417_03405 at 781.494 kb on + strand, within PS417_03405 at 781.495 kb on - strand at 781.620 kb on - strand, within PS417_03405 at 781.621 kb on + strand, within PS417_03405 at 781.621 kb on + strand at 781.621 kb on + strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.622 kb on - strand, within PS417_03405 at 781.623 kb on + strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.624 kb on - strand, within PS417_03405 at 781.778 kb on + strand at 781.817 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 779,041 + PS417_03390 0.86 -1.2 779,041 + PS417_03390 0.86 +0.3 779,041 + PS417_03390 0.86 -1.1 779,042 - PS417_03390 0.86 +0.1 779,042 - PS417_03390 0.86 +0.3 779,042 - PS417_03390 0.86 -0.5 779,189 + -0.5 779,189 + +0.7 779,190 - -1.9 779,190 - +1.7 779,231 + +0.3 779,259 + -2.4 779,259 + -0.1 779,514 - PS417_03395 0.33 -2.1 779,532 + PS417_03395 0.36 -0.5 779,532 + PS417_03395 0.36 +1.7 779,533 - PS417_03395 0.36 +0.5 779,533 - PS417_03395 0.36 +0.1 779,562 + PS417_03395 0.41 -0.5 779,562 + PS417_03395 0.41 -0.1 779,563 - PS417_03395 0.41 +0.6 779,571 + PS417_03395 0.42 +0.6 779,587 + PS417_03395 0.45 -2.3 779,587 + PS417_03395 0.45 +0.8 779,902 - +0.7 779,960 + +0.4 779,961 - -0.2 779,961 - -0.9 780,000 + -0.0 780,007 + -0.1 780,472 + PS417_03400 0.45 -0.8 780,488 - PS417_03400 0.46 -1.9 780,488 - PS417_03400 0.46 -0.3 780,488 - PS417_03400 0.46 -1.4 780,488 - PS417_03400 0.46 +0.1 780,594 - PS417_03400 0.56 -0.4 780,743 + PS417_03400 0.69 -2.3 780,762 - PS417_03400 0.71 -0.5 780,966 - PS417_03400 0.90 -0.1 780,999 - -0.6 781,096 + +0.6 781,168 + PS417_03405 0.14 -1.4 781,168 + PS417_03405 0.14 +3.3 781,168 + PS417_03405 0.14 -0.4 781,405 + PS417_03405 0.46 +0.1 781,405 + PS417_03405 0.46 -1.0 781,406 - PS417_03405 0.47 -0.7 781,406 - PS417_03405 0.47 -0.7 781,494 + PS417_03405 0.59 +0.1 781,494 + PS417_03405 0.59 +0.6 781,495 - +0.8 781,620 - PS417_03405 0.76 -1.8 781,621 + PS417_03405 0.76 +0.3 781,621 + +0.7 781,621 + PS417_03405 0.76 -0.2 781,622 - PS417_03405 0.76 -0.6 781,622 - PS417_03405 0.76 -0.5 781,622 - PS417_03405 0.76 -1.7 781,622 - PS417_03405 0.76 +0.7 781,622 - PS417_03405 0.76 +0.3 781,622 - PS417_03405 0.76 -2.3 781,623 + PS417_03405 0.76 -0.9 781,624 - PS417_03405 0.77 +0.4 781,624 - PS417_03405 0.77 -0.5 781,624 - PS417_03405 0.77 -1.3 781,778 + +1.1 781,817 - -1.5
Or see this region's nucleotide sequence