Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24765 and PS417_24770 overlap by 4 nucleotides PS417_24770 and PS417_24775 are separated by 177 nucleotides PS417_24775 and PS417_24780 are separated by 105 nucleotides
PS417_24765: PS417_24765 - membrane protein, at 5,372,001 to 5,372,717
_24765
PS417_24770: PS417_24770 - murein hydrolase transporter LrgA, at 5,372,714 to 5,373,067
_24770
PS417_24775: PS417_24775 - dehydratase, at 5,373,245 to 5,373,700
_24775
PS417_24780: PS417_24780 - peptidase C13, at 5,373,806 to 5,375,524
_24780
Position (kb) 5373
5374 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5372.394 kb on + strand, within PS417_24765 at 5372.406 kb on - strand, within PS417_24765 at 5372.531 kb on - strand, within PS417_24765 at 5372.639 kb on - strand, within PS417_24765 at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.116 kb on - strand at 5373.175 kb on + strand at 5373.175 kb on + strand at 5373.176 kb on - strand at 5373.176 kb on - strand at 5373.190 kb on + strand at 5373.214 kb on + strand at 5373.214 kb on + strand at 5373.251 kb on + strand at 5373.252 kb on - strand at 5373.252 kb on - strand at 5373.255 kb on + strand at 5373.255 kb on + strand at 5373.255 kb on + strand at 5373.256 kb on - strand at 5373.256 kb on - strand at 5373.331 kb on + strand, within PS417_24775 at 5373.331 kb on + strand, within PS417_24775 at 5373.510 kb on - strand, within PS417_24775 at 5373.510 kb on - strand, within PS417_24775 at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.689 kb on + strand at 5373.723 kb on + strand at 5373.740 kb on + strand at 5373.740 kb on + strand at 5373.741 kb on - strand at 5373.741 kb on - strand at 5373.741 kb on - strand at 5373.744 kb on + strand at 5373.745 kb on - strand at 5373.745 kb on - strand at 5373.751 kb on - strand at 5373.754 kb on + strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,372,394 + PS417_24765 0.55 -0.8 5,372,406 - PS417_24765 0.56 +0.4 5,372,531 - PS417_24765 0.74 -0.6 5,372,639 - PS417_24765 0.89 +2.1 5,373,060 - +1.1 5,373,060 - -2.3 5,373,060 - -0.9 5,373,060 - +0.8 5,373,100 + +0.1 5,373,100 + +1.4 5,373,100 + -0.4 5,373,100 + +0.8 5,373,101 - -0.2 5,373,101 - -0.1 5,373,101 - -0.2 5,373,101 - -1.1 5,373,116 - -0.2 5,373,175 + +1.2 5,373,175 + +1.6 5,373,176 - +0.1 5,373,176 - -0.2 5,373,190 + -0.3 5,373,214 + +0.1 5,373,214 + -0.2 5,373,251 + -0.6 5,373,252 - -0.2 5,373,252 - +0.9 5,373,255 + +1.7 5,373,255 + -1.7 5,373,255 + -0.3 5,373,256 - -0.6 5,373,256 - -2.2 5,373,331 + PS417_24775 0.19 +3.4 5,373,331 + PS417_24775 0.19 -1.4 5,373,510 - PS417_24775 0.58 +0.5 5,373,510 - PS417_24775 0.58 +1.3 5,373,665 + +0.0 5,373,665 + -1.1 5,373,665 + +0.9 5,373,665 + +1.7 5,373,665 + +0.8 5,373,666 - -2.4 5,373,666 - -1.1 5,373,666 - -2.6 5,373,666 - -0.6 5,373,689 + -0.9 5,373,723 + +0.7 5,373,740 + +0.3 5,373,740 + +0.9 5,373,741 - +0.9 5,373,741 - +0.5 5,373,741 - +2.7 5,373,744 + +0.8 5,373,745 - -0.4 5,373,745 - +0.4 5,373,751 - -0.0 5,373,754 + +0.4 5,373,755 - -0.1 5,373,755 - -0.6 5,373,755 - +0.0 5,373,755 - +0.1 5,374,385 + PS417_24780 0.34 -0.1 5,374,385 + PS417_24780 0.34 -0.4 5,374,385 + PS417_24780 0.34 +0.1 5,374,385 + PS417_24780 0.34 +0.7 5,374,385 + PS417_24780 0.34 -0.6 5,374,386 - PS417_24780 0.34 +1.4 5,374,386 - PS417_24780 0.34 -0.0 5,374,386 - PS417_24780 0.34 -0.9 5,374,386 - PS417_24780 0.34 -0.4 5,374,523 + PS417_24780 0.42 -0.1 5,374,523 + PS417_24780 0.42 -0.6 5,374,523 + PS417_24780 0.42 +0.2 5,374,524 - PS417_24780 0.42 +0.3 5,374,524 - PS417_24780 0.42 -2.4 5,374,524 - PS417_24780 0.42 +0.1 5,374,593 - PS417_24780 0.46 +0.5 5,374,593 - PS417_24780 0.46 +0.4 5,374,593 - PS417_24780 0.46 -0.5
Or see this region's nucleotide sequence