Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21995 and PS417_22000 are separated by 169 nucleotides PS417_22000 and PS417_22005 are separated by 14 nucleotides PS417_22005 and PS417_22010 are separated by 141 nucleotides
PS417_21995: PS417_21995 - hypothetical protein, at 4,769,813 to 4,770,046
_21995
PS417_22000: PS417_22000 - glutathione S-transferase, at 4,770,216 to 4,770,848
_22000
PS417_22005: PS417_22005 - epimerase, at 4,770,863 to 4,771,600
_22005
PS417_22010: PS417_22010 - MFS transporter, at 4,771,742 to 4,773,154
_22010
Position (kb) 4770
4771
4772 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4769.994 kb on + strand, within PS417_21995 at 4769.995 kb on - strand, within PS417_21995 at 4769.995 kb on - strand, within PS417_21995 at 4769.995 kb on - strand, within PS417_21995 at 4770.000 kb on + strand, within PS417_21995 at 4770.001 kb on - strand, within PS417_21995 at 4770.010 kb on - strand, within PS417_21995 at 4770.053 kb on - strand at 4770.107 kb on + strand at 4770.113 kb on + strand at 4770.114 kb on - strand at 4770.182 kb on + strand at 4770.182 kb on + strand at 4770.245 kb on + strand at 4770.245 kb on + strand at 4770.528 kb on - strand, within PS417_22000 at 4770.588 kb on + strand, within PS417_22000 at 4770.679 kb on - strand, within PS417_22000 at 4770.730 kb on + strand, within PS417_22000 at 4770.731 kb on - strand, within PS417_22000 at 4770.786 kb on + strand at 4770.787 kb on - strand at 4770.787 kb on - strand at 4770.790 kb on - strand at 4770.790 kb on - strand at 4770.847 kb on - strand at 4770.858 kb on + strand at 4770.879 kb on + strand at 4771.139 kb on + strand, within PS417_22005 at 4771.140 kb on - strand, within PS417_22005 at 4771.310 kb on + strand, within PS417_22005 at 4771.311 kb on - strand, within PS417_22005 at 4771.311 kb on - strand, within PS417_22005 at 4771.596 kb on - strand at 4771.596 kb on - strand at 4771.884 kb on - strand, within PS417_22010 at 4771.904 kb on + strand, within PS417_22010 at 4771.904 kb on + strand, within PS417_22010 at 4771.904 kb on + strand, within PS417_22010 at 4771.905 kb on - strand, within PS417_22010 at 4771.905 kb on - strand, within PS417_22010 at 4771.906 kb on + strand, within PS417_22010 at 4771.906 kb on + strand, within PS417_22010 at 4771.906 kb on + strand, within PS417_22010 at 4771.907 kb on - strand, within PS417_22010 at 4772.090 kb on + strand, within PS417_22010 at 4772.201 kb on + strand, within PS417_22010 at 4772.202 kb on - strand, within PS417_22010 at 4772.380 kb on + strand, within PS417_22010 at 4772.380 kb on + strand, within PS417_22010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,769,994 + PS417_21995 0.77 -0.0 4,769,995 - PS417_21995 0.78 +0.8 4,769,995 - PS417_21995 0.78 -2.9 4,769,995 - PS417_21995 0.78 +0.5 4,770,000 + PS417_21995 0.80 -1.0 4,770,001 - PS417_21995 0.80 +0.7 4,770,010 - PS417_21995 0.84 +0.4 4,770,053 - +0.4 4,770,107 + -0.0 4,770,113 + +0.9 4,770,114 - -0.4 4,770,182 + -0.6 4,770,182 + +0.8 4,770,245 + -2.1 4,770,245 + +0.4 4,770,528 - PS417_22000 0.49 +0.5 4,770,588 + PS417_22000 0.59 +0.4 4,770,679 - PS417_22000 0.73 -0.6 4,770,730 + PS417_22000 0.81 -0.6 4,770,731 - PS417_22000 0.81 -0.0 4,770,786 + -1.1 4,770,787 - +0.5 4,770,787 - +0.1 4,770,790 - +0.0 4,770,790 - -0.6 4,770,847 - -3.6 4,770,858 + +0.1 4,770,879 + +2.5 4,771,139 + PS417_22005 0.37 +0.1 4,771,140 - PS417_22005 0.38 -1.4 4,771,310 + PS417_22005 0.61 -1.1 4,771,311 - PS417_22005 0.61 -2.5 4,771,311 - PS417_22005 0.61 +1.1 4,771,596 - +0.1 4,771,596 - -0.1 4,771,884 - PS417_22010 0.10 +0.2 4,771,904 + PS417_22010 0.11 +0.7 4,771,904 + PS417_22010 0.11 -0.2 4,771,904 + PS417_22010 0.11 +1.8 4,771,905 - PS417_22010 0.12 -0.2 4,771,905 - PS417_22010 0.12 +1.0 4,771,906 + PS417_22010 0.12 -0.3 4,771,906 + PS417_22010 0.12 +0.2 4,771,906 + PS417_22010 0.12 +0.4 4,771,907 - PS417_22010 0.12 +1.0 4,772,090 + PS417_22010 0.25 -0.5 4,772,201 + PS417_22010 0.32 +0.9 4,772,202 - PS417_22010 0.33 +0.4 4,772,380 + PS417_22010 0.45 -0.2 4,772,380 + PS417_22010 0.45 -0.1
Or see this region's nucleotide sequence