Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20720 and PS417_20725 overlap by 1 nucleotides PS417_20725 and PS417_20730 are separated by 6 nucleotides PS417_20730 and PS417_20735 overlap by 4 nucleotides
PS417_20720: PS417_20720 - cbb3-type cytochrome c oxidase subunit I, at 4,503,759 to 4,505,201
_20720
PS417_20725: PS417_20725 - cbb3-type cytochrome c oxidase subunit II, at 4,505,201 to 4,505,809
_20725
PS417_20730: PS417_20730 - cytochrome oxidase, at 4,505,816 to 4,506,001
_20730
PS417_20735: PS417_20735 - cytochrome Cbb3, at 4,505,998 to 4,506,975
_20735
Position (kb)
4505
4506
4507 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4504.848 kb on + strand at 4504.944 kb on + strand, within PS417_20720 at 4504.945 kb on - strand, within PS417_20720 at 4505.061 kb on + strand at 4505.118 kb on + strand at 4505.119 kb on - strand at 4505.376 kb on - strand, within PS417_20725 at 4505.573 kb on + strand, within PS417_20725 at 4505.983 kb on + strand at 4506.020 kb on - strand at 4506.146 kb on - strand, within PS417_20735 at 4506.818 kb on - strand, within PS417_20735 at 4506.929 kb on - strand at 4506.929 kb on - strand at 4506.929 kb on - strand at 4506.935 kb on - strand at 4506.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,504,848 + -2.2 4,504,944 + PS417_20720 0.82 -0.5 4,504,945 - PS417_20720 0.82 +0.3 4,505,061 + +0.2 4,505,118 + -1.4 4,505,119 - -0.4 4,505,376 - PS417_20725 0.29 -0.4 4,505,573 + PS417_20725 0.61 -0.7 4,505,983 + -0.2 4,506,020 - -0.8 4,506,146 - PS417_20735 0.15 +2.4 4,506,818 - PS417_20735 0.84 +0.8 4,506,929 - +0.5 4,506,929 - -0.9 4,506,929 - -0.6 4,506,935 - -0.8 4,506,973 + -1.3
Or see this region's nucleotide sequence