Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20175 and PS417_20180 are separated by 149 nucleotides PS417_20180 and PS417_20185 overlap by 1 nucleotides PS417_20185 and PS417_20190 are separated by 21 nucleotides
PS417_20175: PS417_20175 - LysR family transcriptional regulator, at 4,383,752 to 4,384,645
_20175
PS417_20180: PS417_20180 - agmatinase, at 4,384,795 to 4,385,745
_20180
PS417_20185: PS417_20185 - ketosteroid isomerase, at 4,385,745 to 4,386,167
_20185
PS417_20190: PS417_20190 - nitrate reductase, at 4,386,189 to 4,387,700
_20190
Position (kb)
4384
4385
4386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4383.875 kb on + strand, within PS417_20175 at 4383.912 kb on + strand, within PS417_20175 at 4383.913 kb on - strand, within PS417_20175 at 4383.913 kb on - strand, within PS417_20175 at 4383.936 kb on + strand, within PS417_20175 at 4383.936 kb on + strand, within PS417_20175 at 4383.936 kb on + strand, within PS417_20175 at 4383.936 kb on + strand, within PS417_20175 at 4383.937 kb on - strand, within PS417_20175 at 4383.937 kb on - strand, within PS417_20175 at 4383.937 kb on - strand, within PS417_20175 at 4383.973 kb on + strand, within PS417_20175 at 4383.974 kb on - strand, within PS417_20175 at 4383.974 kb on - strand, within PS417_20175 at 4384.119 kb on + strand, within PS417_20175 at 4384.120 kb on - strand, within PS417_20175 at 4384.152 kb on + strand, within PS417_20175 at 4384.152 kb on + strand, within PS417_20175 at 4384.152 kb on + strand, within PS417_20175 at 4384.152 kb on + strand, within PS417_20175 at 4384.153 kb on - strand, within PS417_20175 at 4384.153 kb on - strand, within PS417_20175 at 4384.262 kb on + strand, within PS417_20175 at 4384.285 kb on - strand, within PS417_20175 at 4384.390 kb on - strand, within PS417_20175 at 4384.527 kb on + strand, within PS417_20175 at 4384.527 kb on + strand, within PS417_20175 at 4384.527 kb on + strand, within PS417_20175 at 4384.527 kb on + strand, within PS417_20175 at 4384.528 kb on - strand, within PS417_20175 at 4384.528 kb on - strand, within PS417_20175 at 4384.677 kb on - strand at 4384.677 kb on - strand at 4384.689 kb on + strand at 4384.689 kb on + strand at 4384.709 kb on + strand at 4384.710 kb on - strand at 4384.710 kb on - strand at 4385.005 kb on + strand, within PS417_20180 at 4385.005 kb on + strand, within PS417_20180 at 4385.077 kb on - strand, within PS417_20180 at 4385.265 kb on + strand, within PS417_20180 at 4385.265 kb on + strand, within PS417_20180 at 4385.266 kb on - strand, within PS417_20180 at 4385.266 kb on - strand, within PS417_20180 at 4385.312 kb on + strand, within PS417_20180 at 4385.372 kb on - strand, within PS417_20180 at 4385.501 kb on - strand, within PS417_20180 at 4385.501 kb on - strand, within PS417_20180 at 4385.501 kb on - strand, within PS417_20180 at 4385.856 kb on + strand, within PS417_20185 at 4385.856 kb on + strand, within PS417_20185 at 4385.857 kb on - strand, within PS417_20185 at 4385.893 kb on - strand, within PS417_20185 at 4386.194 kb on + strand at 4386.194 kb on + strand at 4386.197 kb on + strand at 4386.198 kb on - strand at 4386.209 kb on + strand at 4386.209 kb on + strand at 4386.209 kb on + strand at 4386.210 kb on - strand at 4386.216 kb on - strand at 4386.227 kb on - strand at 4386.267 kb on - strand at 4386.314 kb on + strand at 4386.401 kb on - strand, within PS417_20190 at 4386.401 kb on - strand, within PS417_20190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,383,875 + PS417_20175 0.14 +1.8 4,383,912 + PS417_20175 0.18 -0.5 4,383,913 - PS417_20175 0.18 -2.1 4,383,913 - PS417_20175 0.18 -0.6 4,383,936 + PS417_20175 0.21 +0.4 4,383,936 + PS417_20175 0.21 +0.2 4,383,936 + PS417_20175 0.21 -0.2 4,383,936 + PS417_20175 0.21 +0.3 4,383,937 - PS417_20175 0.21 +0.2 4,383,937 - PS417_20175 0.21 -0.7 4,383,937 - PS417_20175 0.21 +0.7 4,383,973 + PS417_20175 0.25 -1.8 4,383,974 - PS417_20175 0.25 +0.2 4,383,974 - PS417_20175 0.25 +0.3 4,384,119 + PS417_20175 0.41 +0.5 4,384,120 - PS417_20175 0.41 +0.4 4,384,152 + PS417_20175 0.45 -0.0 4,384,152 + PS417_20175 0.45 +1.3 4,384,152 + PS417_20175 0.45 -0.2 4,384,152 + PS417_20175 0.45 -0.0 4,384,153 - PS417_20175 0.45 +2.6 4,384,153 - PS417_20175 0.45 +1.0 4,384,262 + PS417_20175 0.57 +1.0 4,384,285 - PS417_20175 0.60 +0.6 4,384,390 - PS417_20175 0.71 +0.1 4,384,527 + PS417_20175 0.87 -0.6 4,384,527 + PS417_20175 0.87 +1.6 4,384,527 + PS417_20175 0.87 +0.0 4,384,527 + PS417_20175 0.87 +0.0 4,384,528 - PS417_20175 0.87 +2.5 4,384,528 - PS417_20175 0.87 +1.6 4,384,677 - -0.0 4,384,677 - -0.3 4,384,689 + -1.2 4,384,689 + -2.3 4,384,709 + -1.2 4,384,710 - +0.7 4,384,710 - +0.3 4,385,005 + PS417_20180 0.22 -0.9 4,385,005 + PS417_20180 0.22 -0.0 4,385,077 - PS417_20180 0.30 -0.4 4,385,265 + PS417_20180 0.49 -0.8 4,385,265 + PS417_20180 0.49 -1.8 4,385,266 - PS417_20180 0.50 -2.6 4,385,266 - PS417_20180 0.50 +0.2 4,385,312 + PS417_20180 0.54 +0.8 4,385,372 - PS417_20180 0.61 +0.6 4,385,501 - PS417_20180 0.74 -2.0 4,385,501 - PS417_20180 0.74 -0.5 4,385,501 - PS417_20180 0.74 -0.5 4,385,856 + PS417_20185 0.26 -0.2 4,385,856 + PS417_20185 0.26 +0.1 4,385,857 - PS417_20185 0.26 -1.2 4,385,893 - PS417_20185 0.35 +0.7 4,386,194 + +0.9 4,386,194 + +0.0 4,386,197 + -0.7 4,386,198 - +0.1 4,386,209 + -1.2 4,386,209 + +0.1 4,386,209 + -0.1 4,386,210 - +1.1 4,386,216 - +0.3 4,386,227 - -3.3 4,386,267 - +0.1 4,386,314 + -1.7 4,386,401 - PS417_20190 0.14 -0.1 4,386,401 - PS417_20190 0.14 -1.8
Or see this region's nucleotide sequence