Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19940 and PS417_19945 are separated by 61 nucleotides PS417_19945 and PS417_19950 are separated by 121 nucleotides PS417_19950 and PS417_19955 are separated by 266 nucleotides PS417_19955 and PS417_19960 are separated by 20 nucleotides
PS417_19940: PS417_19940 - membrane protein, at 4,328,211 to 4,330,400
_19940
PS417_19945: PS417_19945 - MarR family transcriptional regulator, at 4,330,462 to 4,330,896
_19945
PS417_19950: PS417_19950 - ATP-dependent DNA helicase RecQ, at 4,331,018 to 4,333,144
_19950
PS417_19955: PS417_19955 - hypothetical protein, at 4,333,411 to 4,333,998
_19955
PS417_19960: PS417_19960 - membrane protein, at 4,334,019 to 4,334,402
_19960
Position (kb) 4331
4332
4333
4334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4330.164 kb on + strand, within PS417_19940 at 4330.167 kb on + strand, within PS417_19940 at 4330.215 kb on + strand at 4330.215 kb on + strand at 4330.216 kb on - strand at 4330.219 kb on + strand at 4330.279 kb on - strand at 4330.280 kb on + strand at 4330.280 kb on + strand at 4330.280 kb on + strand at 4330.280 kb on + strand at 4330.280 kb on + strand at 4330.281 kb on - strand at 4330.314 kb on - strand at 4330.342 kb on - strand at 4330.398 kb on + strand at 4330.398 kb on + strand at 4330.617 kb on + strand, within PS417_19945 at 4330.618 kb on - strand, within PS417_19945 at 4330.618 kb on - strand, within PS417_19945 at 4330.618 kb on - strand, within PS417_19945 at 4330.618 kb on - strand, within PS417_19945 at 4330.712 kb on - strand, within PS417_19945 at 4330.938 kb on + strand at 4330.938 kb on + strand at 4330.949 kb on + strand at 4330.987 kb on - strand at 4330.987 kb on - strand at 4331.020 kb on - strand at 4331.690 kb on + strand, within PS417_19950 at 4331.895 kb on + strand, within PS417_19950 at 4332.151 kb on - strand, within PS417_19950 at 4332.208 kb on - strand, within PS417_19950 at 4332.210 kb on + strand, within PS417_19950 at 4332.339 kb on + strand, within PS417_19950 at 4332.445 kb on - strand, within PS417_19950 at 4332.532 kb on + strand, within PS417_19950 at 4332.684 kb on + strand, within PS417_19950 at 4332.685 kb on - strand, within PS417_19950 at 4332.716 kb on + strand, within PS417_19950 at 4332.717 kb on - strand, within PS417_19950 at 4332.810 kb on + strand, within PS417_19950 at 4333.280 kb on + strand at 4333.281 kb on - strand at 4333.334 kb on + strand at 4333.334 kb on + strand at 4333.373 kb on + strand at 4333.402 kb on - strand at 4333.486 kb on + strand, within PS417_19955 at 4333.504 kb on + strand, within PS417_19955 at 4333.940 kb on - strand at 4334.132 kb on - strand, within PS417_19960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,330,164 + PS417_19940 0.89 +0.1 4,330,167 + PS417_19940 0.89 -1.1 4,330,215 + +0.2 4,330,215 + -0.6 4,330,216 - -0.1 4,330,219 + +0.2 4,330,279 - -0.0 4,330,280 + +0.6 4,330,280 + -0.5 4,330,280 + -0.1 4,330,280 + -1.0 4,330,280 + -0.9 4,330,281 - +0.9 4,330,314 - +0.1 4,330,342 - +1.1 4,330,398 + -0.1 4,330,398 + +0.8 4,330,617 + PS417_19945 0.36 -0.2 4,330,618 - PS417_19945 0.36 -0.4 4,330,618 - PS417_19945 0.36 -0.2 4,330,618 - PS417_19945 0.36 -1.5 4,330,618 - PS417_19945 0.36 -0.8 4,330,712 - PS417_19945 0.57 -1.5 4,330,938 + -1.3 4,330,938 + -1.3 4,330,949 + -0.3 4,330,987 - -0.1 4,330,987 - -0.7 4,331,020 - +0.9 4,331,690 + PS417_19950 0.32 +1.4 4,331,895 + PS417_19950 0.41 +2.2 4,332,151 - PS417_19950 0.53 +0.8 4,332,208 - PS417_19950 0.56 +0.6 4,332,210 + PS417_19950 0.56 +0.6 4,332,339 + PS417_19950 0.62 +0.9 4,332,445 - PS417_19950 0.67 -0.3 4,332,532 + PS417_19950 0.71 +1.8 4,332,684 + PS417_19950 0.78 +0.5 4,332,685 - PS417_19950 0.78 -0.7 4,332,716 + PS417_19950 0.80 -0.6 4,332,717 - PS417_19950 0.80 +0.4 4,332,810 + PS417_19950 0.84 +0.4 4,333,280 + -0.4 4,333,281 - -1.4 4,333,334 + +1.1 4,333,334 + -1.5 4,333,373 + +0.3 4,333,402 - -2.1 4,333,486 + PS417_19955 0.13 -3.3 4,333,504 + PS417_19955 0.16 -1.6 4,333,940 - -3.0 4,334,132 - PS417_19960 0.29 +0.1
Or see this region's nucleotide sequence