Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18250 and PS417_18255 are separated by 69 nucleotides PS417_18255 and PS417_18260 are separated by 46 nucleotides PS417_18260 and PS417_18265 are separated by 55 nucleotides
PS417_18250: PS417_18250 - chemotaxis protein, at 3,976,332 to 3,977,624
_18250
PS417_18255: PS417_18255 - AMP-binding protein, at 3,977,694 to 3,979,334
_18255
PS417_18260: PS417_18260 - TetR family transcriptional regulator, at 3,979,381 to 3,979,956
_18260
PS417_18265: PS417_18265 - AsnC family transcriptional regulator, at 3,980,012 to 3,980,494
_18265
Position (kb)
3977
3978
3979
3980 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3976.739 kb on - strand, within PS417_18250 at 3976.903 kb on + strand, within PS417_18250 at 3976.904 kb on - strand, within PS417_18250 at 3977.259 kb on - strand, within PS417_18250 at 3977.272 kb on - strand, within PS417_18250 at 3977.668 kb on + strand at 3977.677 kb on + strand at 3977.678 kb on - strand at 3977.689 kb on - strand at 3978.019 kb on - strand, within PS417_18255 at 3978.272 kb on + strand, within PS417_18255 at 3978.272 kb on + strand, within PS417_18255 at 3978.342 kb on + strand, within PS417_18255 at 3978.344 kb on + strand, within PS417_18255 at 3978.344 kb on + strand, within PS417_18255 at 3978.344 kb on + strand, within PS417_18255 at 3978.345 kb on - strand, within PS417_18255 at 3978.411 kb on + strand, within PS417_18255 at 3978.420 kb on + strand, within PS417_18255 at 3978.421 kb on - strand, within PS417_18255 at 3978.424 kb on - strand, within PS417_18255 at 3978.516 kb on + strand, within PS417_18255 at 3978.517 kb on - strand, within PS417_18255 at 3978.517 kb on - strand, within PS417_18255 at 3979.010 kb on + strand, within PS417_18255 at 3979.011 kb on - strand, within PS417_18255 at 3979.507 kb on + strand, within PS417_18260 at 3979.518 kb on - strand, within PS417_18260 at 3979.518 kb on - strand, within PS417_18260 at 3979.562 kb on - strand, within PS417_18260 at 3979.734 kb on - strand, within PS417_18260 at 3979.766 kb on + strand at 3979.767 kb on - strand, within PS417_18260 at 3979.787 kb on + strand, within PS417_18260 at 3979.963 kb on + strand at 3980.093 kb on + strand, within PS417_18265 at 3980.137 kb on - strand, within PS417_18265 at 3980.137 kb on - strand, within PS417_18265 at 3980.137 kb on - strand, within PS417_18265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,976,739 - PS417_18250 0.31 +1.1 3,976,903 + PS417_18250 0.44 +1.3 3,976,904 - PS417_18250 0.44 -2.0 3,977,259 - PS417_18250 0.72 -1.6 3,977,272 - PS417_18250 0.73 -0.1 3,977,668 + -2.0 3,977,677 + +0.7 3,977,678 - +1.6 3,977,689 - +0.3 3,978,019 - PS417_18255 0.20 -0.9 3,978,272 + PS417_18255 0.35 +0.8 3,978,272 + PS417_18255 0.35 -0.3 3,978,342 + PS417_18255 0.39 -3.0 3,978,344 + PS417_18255 0.40 -1.9 3,978,344 + PS417_18255 0.40 +1.2 3,978,344 + PS417_18255 0.40 -0.7 3,978,345 - PS417_18255 0.40 -0.7 3,978,411 + PS417_18255 0.44 -1.0 3,978,420 + PS417_18255 0.44 -0.8 3,978,421 - PS417_18255 0.44 +0.2 3,978,424 - PS417_18255 0.44 +0.2 3,978,516 + PS417_18255 0.50 -1.1 3,978,517 - PS417_18255 0.50 -0.4 3,978,517 - PS417_18255 0.50 -2.7 3,979,010 + PS417_18255 0.80 +0.1 3,979,011 - PS417_18255 0.80 +0.4 3,979,507 + PS417_18260 0.22 +0.5 3,979,518 - PS417_18260 0.24 -0.3 3,979,518 - PS417_18260 0.24 +0.1 3,979,562 - PS417_18260 0.31 +0.1 3,979,734 - PS417_18260 0.61 +0.9 3,979,766 + +1.4 3,979,767 - PS417_18260 0.67 +1.1 3,979,787 + PS417_18260 0.70 -0.6 3,979,963 + +1.9 3,980,093 + PS417_18265 0.17 +1.0 3,980,137 - PS417_18265 0.26 -0.5 3,980,137 - PS417_18265 0.26 -0.6 3,980,137 - PS417_18265 0.26 -0.5
Or see this region's nucleotide sequence