Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15525 and PS417_15530 overlap by 10 nucleotides PS417_15530 and PS417_15535 are separated by 36 nucleotides PS417_15535 and PS417_15540 are separated by 127 nucleotides PS417_15540 and PS417_15545 are separated by 44 nucleotides
PS417_15525: PS417_15525 - AraC family transcriptional regulator, at 3,398,766 to 3,399,632
_15525
PS417_15530: PS417_15530 - ribonuclease, at 3,399,623 to 3,400,252
_15530
PS417_15535: PS417_15535 - oxidoreductase, at 3,400,289 to 3,401,269
_15535
PS417_15540: PS417_15540 - NUDIX hydrolase, at 3,401,397 to 3,401,759
_15540
PS417_15545: PS417_15545 - glucosidase, at 3,401,804 to 3,404,440
_15545
Position (kb)
3400
3401
3402 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3399.646 kb on + strand at 3399.825 kb on + strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.826 kb on - strand, within PS417_15530 at 3399.928 kb on + strand, within PS417_15530 at 3399.929 kb on - strand, within PS417_15530 at 3400.053 kb on + strand, within PS417_15530 at 3400.065 kb on - strand, within PS417_15530 at 3400.076 kb on + strand, within PS417_15530 at 3400.077 kb on - strand, within PS417_15530 at 3400.077 kb on - strand, within PS417_15530 at 3400.286 kb on + strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.287 kb on - strand at 3400.290 kb on + strand at 3400.316 kb on + strand at 3400.317 kb on - strand at 3400.692 kb on + strand, within PS417_15535 at 3400.692 kb on + strand, within PS417_15535 at 3400.734 kb on + strand, within PS417_15535 at 3401.026 kb on - strand, within PS417_15535 at 3401.059 kb on - strand at 3401.107 kb on - strand, within PS417_15535 at 3401.199 kb on + strand at 3401.339 kb on + strand at 3401.404 kb on + strand at 3401.662 kb on + strand, within PS417_15540 at 3401.686 kb on - strand, within PS417_15540 at 3401.757 kb on + strand at 3401.758 kb on - strand at 3401.758 kb on - strand at 3401.758 kb on - strand at 3401.823 kb on - strand at 3402.130 kb on - strand, within PS417_15545 at 3402.130 kb on - strand, within PS417_15545 at 3402.207 kb on + strand, within PS417_15545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,399,646 + +0.6 3,399,825 + PS417_15530 0.32 -1.4 3,399,826 - PS417_15530 0.32 +0.2 3,399,826 - PS417_15530 0.32 -0.7 3,399,826 - PS417_15530 0.32 -0.7 3,399,928 + PS417_15530 0.48 +0.1 3,399,929 - PS417_15530 0.49 -0.1 3,400,053 + PS417_15530 0.68 -0.2 3,400,065 - PS417_15530 0.70 +1.2 3,400,076 + PS417_15530 0.72 -0.2 3,400,077 - PS417_15530 0.72 -0.6 3,400,077 - PS417_15530 0.72 -0.0 3,400,286 + +0.1 3,400,287 - -0.7 3,400,287 - +0.1 3,400,287 - -2.3 3,400,287 - -0.6 3,400,290 + +1.1 3,400,316 + -0.5 3,400,317 - +0.1 3,400,692 + PS417_15535 0.41 +1.2 3,400,692 + PS417_15535 0.41 +0.9 3,400,734 + PS417_15535 0.45 +0.3 3,401,026 - PS417_15535 0.75 +0.3 3,401,059 - +0.8 3,401,107 - PS417_15535 0.83 +2.0 3,401,199 + -0.0 3,401,339 + -0.8 3,401,404 + +1.3 3,401,662 + PS417_15540 0.73 -1.0 3,401,686 - PS417_15540 0.80 +0.6 3,401,757 + +0.2 3,401,758 - -0.1 3,401,758 - +0.6 3,401,758 - +0.4 3,401,823 - +0.3 3,402,130 - PS417_15545 0.12 -0.5 3,402,130 - PS417_15545 0.12 -0.3 3,402,207 + PS417_15545 0.15 +0.2
Or see this region's nucleotide sequence