Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14285 and PS417_14290 are separated by 148 nucleotides PS417_14290 and PS417_14295 are separated by 214 nucleotides
PS417_14285: PS417_14285 - hypothetical protein, at 3,125,435 to 3,126,211
_14285
PS417_14290: PS417_14290 - trehalose synthase, at 3,126,360 to 3,128,423
_14290
PS417_14295: PS417_14295 - AraC family transcriptional regulator, at 3,128,638 to 3,129,396
_14295
Position (kb)
3126
3127
3128
3129 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3125.422 kb on + strand at 3125.422 kb on + strand at 3125.504 kb on - strand at 3125.516 kb on + strand, within PS417_14285 at 3125.517 kb on - strand, within PS417_14285 at 3125.782 kb on - strand, within PS417_14285 at 3125.917 kb on - strand, within PS417_14285 at 3125.919 kb on + strand, within PS417_14285 at 3125.919 kb on + strand, within PS417_14285 at 3125.919 kb on + strand, within PS417_14285 at 3125.920 kb on - strand, within PS417_14285 at 3125.984 kb on - strand, within PS417_14285 at 3126.086 kb on + strand, within PS417_14285 at 3126.149 kb on + strand at 3126.177 kb on - strand at 3126.236 kb on + strand at 3126.251 kb on - strand at 3126.291 kb on + strand at 3126.404 kb on - strand at 3126.422 kb on + strand at 3126.650 kb on - strand, within PS417_14290 at 3126.752 kb on + strand, within PS417_14290 at 3126.753 kb on - strand, within PS417_14290 at 3126.929 kb on - strand, within PS417_14290 at 3126.929 kb on - strand, within PS417_14290 at 3126.929 kb on - strand, within PS417_14290 at 3126.929 kb on - strand, within PS417_14290 at 3127.099 kb on + strand, within PS417_14290 at 3127.100 kb on - strand, within PS417_14290 at 3127.100 kb on - strand, within PS417_14290 at 3127.422 kb on - strand, within PS417_14290 at 3127.469 kb on + strand, within PS417_14290 at 3127.663 kb on + strand, within PS417_14290 at 3127.672 kb on + strand, within PS417_14290 at 3127.786 kb on + strand, within PS417_14290 at 3127.786 kb on + strand, within PS417_14290 at 3127.787 kb on - strand, within PS417_14290 at 3127.921 kb on + strand, within PS417_14290 at 3127.921 kb on + strand, within PS417_14290 at 3127.922 kb on - strand, within PS417_14290 at 3128.259 kb on - strand at 3128.432 kb on + strand at 3128.445 kb on - strand at 3128.464 kb on + strand at 3128.464 kb on + strand at 3128.464 kb on + strand at 3128.464 kb on + strand at 3128.465 kb on - strand at 3128.465 kb on - strand at 3128.465 kb on - strand at 3128.570 kb on + strand at 3128.570 kb on + strand at 3128.570 kb on + strand at 3128.571 kb on - strand at 3129.026 kb on + strand, within PS417_14295 at 3129.027 kb on - strand, within PS417_14295 at 3129.313 kb on + strand, within PS417_14295 at 3129.314 kb on - strand, within PS417_14295 at 3129.334 kb on + strand at 3129.334 kb on + strand at 3129.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 3,125,422 + +0.5 3,125,422 + +0.6 3,125,504 - +2.2 3,125,516 + PS417_14285 0.10 -0.7 3,125,517 - PS417_14285 0.11 +0.6 3,125,782 - PS417_14285 0.45 +0.4 3,125,917 - PS417_14285 0.62 -1.1 3,125,919 + PS417_14285 0.62 +1.4 3,125,919 + PS417_14285 0.62 -0.7 3,125,919 + PS417_14285 0.62 +0.6 3,125,920 - PS417_14285 0.62 +0.6 3,125,984 - PS417_14285 0.71 +1.0 3,126,086 + PS417_14285 0.84 -0.2 3,126,149 + -0.2 3,126,177 - +1.3 3,126,236 + -0.2 3,126,251 - -0.4 3,126,291 + -3.2 3,126,404 - +0.7 3,126,422 + +0.4 3,126,650 - PS417_14290 0.14 -0.9 3,126,752 + PS417_14290 0.19 +0.4 3,126,753 - PS417_14290 0.19 -2.1 3,126,929 - PS417_14290 0.28 +1.7 3,126,929 - PS417_14290 0.28 -0.0 3,126,929 - PS417_14290 0.28 +0.4 3,126,929 - PS417_14290 0.28 +0.4 3,127,099 + PS417_14290 0.36 +3.2 3,127,100 - PS417_14290 0.36 -0.6 3,127,100 - PS417_14290 0.36 -0.3 3,127,422 - PS417_14290 0.51 +1.4 3,127,469 + PS417_14290 0.54 -0.3 3,127,663 + PS417_14290 0.63 +0.8 3,127,672 + PS417_14290 0.64 +1.4 3,127,786 + PS417_14290 0.69 -0.1 3,127,786 + PS417_14290 0.69 -1.2 3,127,787 - PS417_14290 0.69 +0.2 3,127,921 + PS417_14290 0.76 -2.2 3,127,921 + PS417_14290 0.76 +1.5 3,127,922 - PS417_14290 0.76 +0.8 3,128,259 - -1.1 3,128,432 + -0.2 3,128,445 - +0.1 3,128,464 + +0.4 3,128,464 + +0.0 3,128,464 + +0.0 3,128,464 + -2.0 3,128,465 - -0.1 3,128,465 - +0.1 3,128,465 - -0.4 3,128,570 + +1.0 3,128,570 + +0.1 3,128,570 + -0.5 3,128,571 - -2.7 3,129,026 + PS417_14295 0.51 +1.5 3,129,027 - PS417_14295 0.51 -1.2 3,129,313 + PS417_14295 0.89 +1.1 3,129,314 - PS417_14295 0.89 -0.3 3,129,334 + -0.0 3,129,334 + -0.2 3,129,335 - -0.6
Or see this region's nucleotide sequence