Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01395 and PS417_01400 are separated by 26 nucleotides PS417_01400 and PS417_01405 are separated by 5 nucleotides PS417_01405 and PS417_01410 overlap by 23 nucleotides PS417_01410 and PS417_01415 overlap by 11 nucleotides
PS417_01395: PS417_01395 - biofilm formation protein PelB, at 306,182 to 309,781
_01395
PS417_01400: PS417_01400 - hypothetical protein, at 309,808 to 310,326
_01400
PS417_01405: PS417_01405 - sugar transporter, at 310,332 to 311,699
_01405
PS417_01410: PS417_01410 - transporter, at 311,677 to 312,672
_01410
PS417_01415: PS417_01415 - glycosyl transferase family 1, at 312,662 to 314,176
_01415
Position (kb)
310
311
312 Strain fitness (log2 ratio)
-2
-1
0
1 at 309.356 kb on + strand, within PS417_01395 at 309.357 kb on - strand, within PS417_01395 at 309.392 kb on + strand, within PS417_01395 at 309.393 kb on - strand at 309.393 kb on - strand, within PS417_01395 at 309.393 kb on - strand, within PS417_01395 at 309.519 kb on - strand at 309.519 kb on - strand at 309.578 kb on + strand at 309.579 kb on - strand at 309.635 kb on + strand at 309.635 kb on + strand at 309.636 kb on - strand at 309.636 kb on - strand at 309.758 kb on + strand at 309.793 kb on - strand at 309.845 kb on + strand at 309.846 kb on - strand at 309.938 kb on - strand, within PS417_01400 at 310.148 kb on - strand, within PS417_01400 at 310.403 kb on - strand at 310.599 kb on + strand, within PS417_01405 at 310.602 kb on - strand at 310.714 kb on - strand, within PS417_01405 at 311.596 kb on + strand at 311.649 kb on - strand at 311.649 kb on - strand at 311.659 kb on - strand at 311.659 kb on - strand at 311.950 kb on + strand, within PS417_01410 at 311.951 kb on - strand, within PS417_01410 at 311.951 kb on - strand, within PS417_01410 at 311.951 kb on - strand, within PS417_01410 at 312.323 kb on - strand, within PS417_01410 at 312.577 kb on + strand at 312.671 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 309,356 + PS417_01395 0.88 -1.0 309,357 - PS417_01395 0.88 -1.7 309,392 + PS417_01395 0.89 -0.5 309,393 - -0.1 309,393 - PS417_01395 0.89 -0.0 309,393 - PS417_01395 0.89 +0.0 309,519 - +0.4 309,519 - -0.5 309,578 + +0.5 309,579 - -0.1 309,635 + -1.4 309,635 + -1.6 309,636 - -1.7 309,636 - -0.7 309,758 + +0.4 309,793 - +0.0 309,845 + -0.2 309,846 - -0.5 309,938 - PS417_01400 0.25 +0.2 310,148 - PS417_01400 0.66 -0.2 310,403 - +1.3 310,599 + PS417_01405 0.20 -0.9 310,602 - -0.9 310,714 - PS417_01405 0.28 -1.6 311,596 + -0.7 311,649 - -0.4 311,649 - +0.2 311,659 - -1.1 311,659 - -0.9 311,950 + PS417_01410 0.27 +0.1 311,951 - PS417_01410 0.28 -0.1 311,951 - PS417_01410 0.28 -2.4 311,951 - PS417_01410 0.28 -0.4 312,323 - PS417_01410 0.65 -0.5 312,577 + -1.2 312,671 - -1.8
Or see this region's nucleotide sequence