Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01380 and PS417_01385 are separated by 519 nucleotides PS417_01385 and PS417_01390 are separated by 62 nucleotides
PS417_01380: PS417_01380 - nucleotide cyclase, at 300,536 to 301,876
_01380
PS417_01385: PS417_01385 - NAD-dependent dehydratase, at 302,396 to 303,328
_01385
PS417_01390: PS417_01390 - PbsX family transcriptional regulator, at 303,391 to 306,204
_01390
Position (kb) 302
303
304 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 301.526 kb on + strand, within PS417_01380 at 301.625 kb on + strand, within PS417_01380 at 301.856 kb on - strand at 301.966 kb on + strand at 301.967 kb on - strand at 301.967 kb on - strand at 301.967 kb on - strand at 301.967 kb on - strand at 302.029 kb on - strand at 302.081 kb on + strand at 302.081 kb on + strand at 302.082 kb on - strand at 302.341 kb on + strand at 302.382 kb on - strand at 302.404 kb on - strand at 302.438 kb on - strand at 302.477 kb on + strand at 302.512 kb on + strand, within PS417_01385 at 302.832 kb on - strand, within PS417_01385 at 302.859 kb on - strand, within PS417_01385 at 302.859 kb on - strand at 302.861 kb on + strand, within PS417_01385 at 302.874 kb on - strand, within PS417_01385 at 303.007 kb on + strand, within PS417_01385 at 303.008 kb on - strand, within PS417_01385 at 303.048 kb on - strand, within PS417_01385 at 303.120 kb on + strand, within PS417_01385 at 303.201 kb on - strand, within PS417_01385 at 303.245 kb on - strand at 303.326 kb on + strand at 303.326 kb on + strand at 303.326 kb on + strand at 303.354 kb on + strand at 303.354 kb on + strand at 303.355 kb on - strand at 303.362 kb on - strand at 303.386 kb on - strand at 303.495 kb on - strand at 303.643 kb on + strand at 303.644 kb on - strand at 303.817 kb on + strand, within PS417_01390 at 304.139 kb on - strand, within PS417_01390
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 301,526 + PS417_01380 0.74 -0.7 301,625 + PS417_01380 0.81 +0.6 301,856 - -0.9 301,966 + +0.8 301,967 - -0.3 301,967 - -0.2 301,967 - +0.6 301,967 - -2.9 302,029 - +1.2 302,081 + +0.3 302,081 + -1.3 302,082 - -2.0 302,341 + -1.5 302,382 - +0.6 302,404 - -0.7 302,438 - -0.6 302,477 + -0.4 302,512 + PS417_01385 0.12 -0.6 302,832 - PS417_01385 0.47 -2.1 302,859 - PS417_01385 0.50 -0.3 302,859 - -0.6 302,861 + PS417_01385 0.50 +1.1 302,874 - PS417_01385 0.51 +2.1 303,007 + PS417_01385 0.65 +2.0 303,008 - PS417_01385 0.66 -0.6 303,048 - PS417_01385 0.70 -0.0 303,120 + PS417_01385 0.78 +3.4 303,201 - PS417_01385 0.86 -0.5 303,245 - -1.4 303,326 + -0.6 303,326 + -1.3 303,326 + +0.3 303,354 + -0.1 303,354 + +0.7 303,355 - +0.1 303,362 - -1.1 303,386 - -1.9 303,495 - -0.3 303,643 + -0.7 303,644 - -0.5 303,817 + PS417_01390 0.15 -0.8 304,139 - PS417_01390 0.27 -2.9
Or see this region's nucleotide sequence