Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13270 and PS417_13275 overlap by 4 nucleotides PS417_13275 and PS417_13280 are separated by 33 nucleotides
PS417_13270: PS417_13270 - lipoprotein, at 2,913,521 to 2,914,405
_13270
PS417_13275: PS417_13275 - membrane protein, at 2,914,402 to 2,917,455
_13275
PS417_13280: PS417_13280 - TonB-dependent receptor, at 2,917,489 to 2,919,636
_13280
Position (kb)
2914
2915
2916
2917
2918 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2913.623 kb on + strand, within PS417_13270 at 2913.624 kb on - strand, within PS417_13270 at 2913.624 kb on - strand, within PS417_13270 at 2913.730 kb on - strand, within PS417_13270 at 2913.750 kb on + strand, within PS417_13270 at 2913.751 kb on - strand at 2913.936 kb on - strand, within PS417_13270 at 2914.042 kb on + strand, within PS417_13270 at 2914.043 kb on - strand, within PS417_13270 at 2914.358 kb on - strand at 2914.358 kb on - strand at 2914.361 kb on + strand at 2914.361 kb on + strand at 2914.361 kb on + strand at 2914.362 kb on - strand at 2914.362 kb on - strand at 2914.364 kb on + strand at 2914.408 kb on - strand at 2914.501 kb on + strand at 2914.532 kb on - strand at 2914.586 kb on - strand at 2914.685 kb on + strand at 2914.685 kb on + strand at 2914.918 kb on + strand at 2915.067 kb on + strand, within PS417_13275 at 2915.198 kb on + strand, within PS417_13275 at 2915.354 kb on + strand, within PS417_13275 at 2916.532 kb on + strand, within PS417_13275 at 2916.533 kb on - strand, within PS417_13275 at 2916.660 kb on + strand, within PS417_13275 at 2917.093 kb on + strand, within PS417_13275 at 2917.693 kb on - strand at 2918.050 kb on + strand, within PS417_13280 at 2918.051 kb on - strand, within PS417_13280 at 2918.351 kb on - strand, within PS417_13280 at 2918.453 kb on - strand, within PS417_13280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,913,623 + PS417_13270 0.12 +1.0 2,913,624 - PS417_13270 0.12 +0.2 2,913,624 - PS417_13270 0.12 -0.3 2,913,730 - PS417_13270 0.24 -0.7 2,913,750 + PS417_13270 0.26 -0.5 2,913,751 - +1.4 2,913,936 - PS417_13270 0.47 -1.5 2,914,042 + PS417_13270 0.59 +0.1 2,914,043 - PS417_13270 0.59 +0.9 2,914,358 - -0.1 2,914,358 - +1.9 2,914,361 + +1.4 2,914,361 + -0.8 2,914,361 + +0.2 2,914,362 - +0.3 2,914,362 - -0.0 2,914,364 + +0.1 2,914,408 - +0.7 2,914,501 + +3.0 2,914,532 - -0.1 2,914,586 - +0.6 2,914,685 + -0.6 2,914,685 + +0.4 2,914,918 + +2.0 2,915,067 + PS417_13275 0.22 -0.3 2,915,198 + PS417_13275 0.26 +0.6 2,915,354 + PS417_13275 0.31 +2.4 2,916,532 + PS417_13275 0.70 +1.1 2,916,533 - PS417_13275 0.70 +0.6 2,916,660 + PS417_13275 0.74 +1.5 2,917,093 + PS417_13275 0.88 -0.9 2,917,693 - +0.1 2,918,050 + PS417_13280 0.26 +0.3 2,918,051 - PS417_13280 0.26 -0.1 2,918,351 - PS417_13280 0.40 +0.8 2,918,453 - PS417_13280 0.45 -0.3
Or see this region's nucleotide sequence