Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01295 and PS417_01300 are separated by 126 nucleotides PS417_01300 and PS417_01305 are separated by 26 nucleotides PS417_01305 and PS417_01310 overlap by 4 nucleotides
PS417_01295: PS417_01295 - adenosine-3'(2'),5'-bisphosphate nucleotidase, at 280,798 to 281,634
_01295
PS417_01300: PS417_01300 - thioesterase, at 281,761 to 282,216
_01300
PS417_01305: PS417_01305 - Fis family transcriptional regulator, at 282,243 to 283,619
_01305
PS417_01310: PS417_01310 - ATPase, at 283,616 to 285,424
_01310
Position (kb) 282
283
284 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 281.577 kb on - strand at 281.645 kb on - strand at 281.778 kb on - strand at 281.778 kb on - strand at 281.867 kb on + strand, within PS417_01300 at 281.868 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 281.977 kb on - strand, within PS417_01300 at 282.291 kb on + strand at 282.291 kb on + strand at 282.291 kb on + strand at 282.428 kb on + strand at 282.542 kb on + strand at 282.542 kb on + strand, within PS417_01305 at 282.701 kb on - strand, within PS417_01305 at 282.703 kb on + strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 282.704 kb on - strand, within PS417_01305 at 283.315 kb on + strand, within PS417_01305 at 283.619 kb on + strand at 283.619 kb on + strand at 283.619 kb on + strand at 283.620 kb on - strand at 283.620 kb on - strand at 283.620 kb on - strand at 283.800 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310 at 283.863 kb on + strand, within PS417_01310
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 281,577 - -1.9 281,645 - +0.6 281,778 - +1.4 281,778 - +0.4 281,867 + PS417_01300 0.23 +0.7 281,868 - PS417_01300 0.23 -1.9 281,977 - PS417_01300 0.47 -1.4 281,977 - PS417_01300 0.47 +0.0 281,977 - PS417_01300 0.47 +0.4 281,977 - PS417_01300 0.47 -1.0 281,977 - PS417_01300 0.47 -0.6 282,291 + +0.3 282,291 + +0.9 282,291 + -1.0 282,428 + +0.4 282,542 + -0.9 282,542 + PS417_01305 0.22 +6.3 282,701 - PS417_01305 0.33 +1.1 282,703 + PS417_01305 0.33 +2.5 282,704 - PS417_01305 0.33 +0.1 282,704 - PS417_01305 0.33 -0.3 282,704 - PS417_01305 0.33 +2.0 283,315 + PS417_01305 0.78 -1.2 283,619 + +0.2 283,619 + +0.2 283,619 + -0.4 283,620 - -0.5 283,620 - +0.6 283,620 - -1.0 283,800 + PS417_01310 0.10 +1.1 283,863 + PS417_01310 0.14 +3.7 283,863 + PS417_01310 0.14 +0.5 283,863 + PS417_01310 0.14 -1.1
Or see this region's nucleotide sequence