Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12895 and PS417_12900 are separated by 77 nucleotides PS417_12900 and PS417_12905 are separated by 100 nucleotides
PS417_12895: PS417_12895 - pyruvate dehydrogenase, at 2,819,736 to 2,822,402
_12895
PS417_12900: PS417_12900 - Fe-S protein, at 2,822,480 to 2,822,968
_12900
PS417_12905: PS417_12905 - ribonucleotide-diphosphate reductase subunit beta, at 2,823,069 to 2,824,319
_12905
Position (kb)
2822
2823 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2821.536 kb on + strand, within PS417_12895 at 2821.537 kb on - strand, within PS417_12895 at 2821.537 kb on - strand, within PS417_12895 at 2821.537 kb on - strand, within PS417_12895 at 2821.537 kb on - strand, within PS417_12895 at 2821.537 kb on - strand, within PS417_12895 at 2821.542 kb on + strand, within PS417_12895 at 2821.542 kb on + strand at 2821.542 kb on + strand, within PS417_12895 at 2821.542 kb on + strand, within PS417_12895 at 2821.542 kb on + strand, within PS417_12895 at 2821.543 kb on - strand, within PS417_12895 at 2821.543 kb on - strand, within PS417_12895 at 2821.543 kb on - strand, within PS417_12895 at 2821.546 kb on - strand, within PS417_12895 at 2821.806 kb on + strand, within PS417_12895 at 2821.806 kb on + strand, within PS417_12895 at 2821.807 kb on - strand, within PS417_12895 at 2821.807 kb on - strand, within PS417_12895 at 2821.807 kb on - strand, within PS417_12895 at 2821.809 kb on + strand, within PS417_12895 at 2821.809 kb on + strand, within PS417_12895 at 2821.810 kb on - strand, within PS417_12895 at 2821.887 kb on + strand, within PS417_12895 at 2821.887 kb on + strand, within PS417_12895 at 2821.888 kb on - strand, within PS417_12895 at 2821.888 kb on - strand, within PS417_12895 at 2822.151 kb on + strand at 2822.151 kb on + strand at 2822.152 kb on - strand at 2822.184 kb on + strand at 2822.184 kb on + strand at 2822.185 kb on - strand at 2822.185 kb on - strand at 2822.185 kb on - strand at 2822.185 kb on - strand at 2822.538 kb on + strand, within PS417_12900 at 2822.538 kb on + strand, within PS417_12900 at 2822.538 kb on + strand, within PS417_12900 at 2822.538 kb on + strand, within PS417_12900 at 2822.539 kb on - strand, within PS417_12900 at 2822.692 kb on - strand, within PS417_12900 at 2822.980 kb on - strand at 2823.063 kb on + strand at 2823.101 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,821,536 + PS417_12895 0.67 +0.4 2,821,537 - PS417_12895 0.68 +1.5 2,821,537 - PS417_12895 0.68 -0.1 2,821,537 - PS417_12895 0.68 -0.2 2,821,537 - PS417_12895 0.68 +1.1 2,821,537 - PS417_12895 0.68 -0.0 2,821,542 + PS417_12895 0.68 +0.2 2,821,542 + -1.8 2,821,542 + PS417_12895 0.68 +0.0 2,821,542 + PS417_12895 0.68 +1.0 2,821,542 + PS417_12895 0.68 -0.2 2,821,543 - PS417_12895 0.68 +1.0 2,821,543 - PS417_12895 0.68 -0.6 2,821,543 - PS417_12895 0.68 +0.8 2,821,546 - PS417_12895 0.68 -0.3 2,821,806 + PS417_12895 0.78 -0.6 2,821,806 + PS417_12895 0.78 -0.4 2,821,807 - PS417_12895 0.78 -0.8 2,821,807 - PS417_12895 0.78 -0.4 2,821,807 - PS417_12895 0.78 +0.1 2,821,809 + PS417_12895 0.78 -1.6 2,821,809 + PS417_12895 0.78 +0.0 2,821,810 - PS417_12895 0.78 -0.1 2,821,887 + PS417_12895 0.81 -2.3 2,821,887 + PS417_12895 0.81 -1.2 2,821,888 - PS417_12895 0.81 -2.2 2,821,888 - PS417_12895 0.81 +0.2 2,822,151 + +1.8 2,822,151 + +0.5 2,822,152 - -0.6 2,822,184 + -3.2 2,822,184 + +0.8 2,822,185 - +0.1 2,822,185 - +2.3 2,822,185 - +0.5 2,822,185 - -0.4 2,822,538 + PS417_12900 0.12 -0.3 2,822,538 + PS417_12900 0.12 -1.3 2,822,538 + PS417_12900 0.12 -1.3 2,822,538 + PS417_12900 0.12 -0.4 2,822,539 - PS417_12900 0.12 -0.0 2,822,692 - PS417_12900 0.43 -1.9 2,822,980 - -0.6 2,823,063 + +0.1 2,823,101 - -0.8
Or see this region's nucleotide sequence