Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00120 and PS417_00125 overlap by 1 nucleotides PS417_00125 and PS417_00130 are separated by 36 nucleotides PS417_00130 and PS417_00135 are separated by 133 nucleotides
PS417_00120: PS417_00120 - coproporphyrinogen III oxidase, at 25,508 to 26,440
_00120
PS417_00125: PS417_00125 - shikimate dehydrogenase, at 26,440 to 27,258
_00125
PS417_00130: PS417_00130 - SulP family inorganic anion transporter, at 27,295 to 28,887
_00130
PS417_00135: PS417_00135 - glycine/betaine ABC transporter substrate-binding protein, at 29,021 to 29,935
_00135
Position (kb) 27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 26.536 kb on + strand, within PS417_00125 at 27.190 kb on + strand at 27.263 kb on - strand at 27.263 kb on - strand at 27.468 kb on - strand, within PS417_00130 at 27.468 kb on - strand, within PS417_00130 at 27.542 kb on + strand, within PS417_00130 at 27.542 kb on + strand, within PS417_00130 at 28.283 kb on + strand, within PS417_00130 at 28.283 kb on + strand, within PS417_00130 at 28.283 kb on + strand, within PS417_00130 at 28.284 kb on - strand, within PS417_00130 at 28.580 kb on + strand, within PS417_00130 at 28.580 kb on + strand, within PS417_00130 at 28.581 kb on - strand, within PS417_00130 at 28.581 kb on - strand, within PS417_00130 at 28.761 kb on + strand at 28.898 kb on + strand at 28.899 kb on - strand at 28.902 kb on + strand at 28.902 kb on + strand at 28.902 kb on + strand at 28.902 kb on + strand at 28.903 kb on - strand at 28.903 kb on - strand at 28.907 kb on + strand at 28.907 kb on + strand at 29.001 kb on + strand at 29.001 kb on + strand at 29.002 kb on - strand at 29.002 kb on - strand at 29.002 kb on - strand at 29.015 kb on + strand at 29.015 kb on + strand at 29.036 kb on + strand at 29.090 kb on - strand at 29.263 kb on + strand at 29.264 kb on - strand, within PS417_00135 at 29.265 kb on + strand, within PS417_00135 at 29.266 kb on - strand, within PS417_00135 at 29.486 kb on - strand, within PS417_00135 at 29.514 kb on + strand, within PS417_00135 at 29.542 kb on - strand, within PS417_00135 at 29.583 kb on - strand, within PS417_00135 at 29.583 kb on - strand, within PS417_00135 at 29.583 kb on - strand, within PS417_00135 at 29.586 kb on + strand, within PS417_00135 at 29.671 kb on - strand, within PS417_00135 at 29.671 kb on - strand, within PS417_00135 at 29.779 kb on - strand, within PS417_00135 at 29.855 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 26,536 + PS417_00125 0.12 -0.1 27,190 + +2.8 27,263 - -0.8 27,263 - +0.1 27,468 - PS417_00130 0.11 -1.9 27,468 - PS417_00130 0.11 +1.1 27,542 + PS417_00130 0.16 +0.7 27,542 + PS417_00130 0.16 +0.5 28,283 + PS417_00130 0.62 +0.7 28,283 + PS417_00130 0.62 -0.7 28,283 + PS417_00130 0.62 +0.8 28,284 - PS417_00130 0.62 -1.4 28,580 + PS417_00130 0.81 +0.3 28,580 + PS417_00130 0.81 -1.1 28,581 - PS417_00130 0.81 +2.2 28,581 - PS417_00130 0.81 +1.0 28,761 + -0.1 28,898 + -0.6 28,899 - +1.0 28,902 + +0.9 28,902 + +0.1 28,902 + -0.8 28,902 + -0.1 28,903 - -0.5 28,903 - -0.8 28,907 + +0.3 28,907 + -1.0 29,001 + +1.4 29,001 + -0.1 29,002 - -2.1 29,002 - -0.9 29,002 - -1.4 29,015 + +1.1 29,015 + +0.9 29,036 + +0.5 29,090 - -3.1 29,263 + -1.1 29,264 - PS417_00135 0.27 -1.6 29,265 + PS417_00135 0.27 -0.9 29,266 - PS417_00135 0.27 -0.5 29,486 - PS417_00135 0.51 +0.3 29,514 + PS417_00135 0.54 -0.6 29,542 - PS417_00135 0.57 +1.2 29,583 - PS417_00135 0.61 +1.3 29,583 - PS417_00135 0.61 -0.1 29,583 - PS417_00135 0.61 +0.6 29,586 + PS417_00135 0.62 +0.3 29,671 - PS417_00135 0.71 -0.3 29,671 - PS417_00135 0.71 -0.1 29,779 - PS417_00135 0.83 +0.5 29,855 + +0.2
Or see this region's nucleotide sequence