Strain Fitness in Pseudomonas simiae WCS417 around PS417_12600

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_12590 and PS417_12595 are separated by 287 nucleotidesPS417_12595 and PS417_12600 are separated by 3 nucleotidesPS417_12600 and PS417_12605 are separated by 0 nucleotidesPS417_12605 and PS417_12610 overlap by 4 nucleotides PS417_12590: PS417_12590 - RND transporter, at 2,741,033 to 2,742,175 _12590 PS417_12595: PS417_12595 - transcriptional regulator, at 2,742,463 to 2,743,164 _12595 PS417_12600: PS417_12600 - histidine kinase, at 2,743,168 to 2,744,469 _12600 PS417_12605: PS417_12605 - RNA polymerase subunit sigma-70, at 2,744,470 to 2,745,138 _12605 PS417_12610: PS417_12610 - RNA polymerase sigma factor, at 2,745,135 to 2,745,650 _12610 Position (kb) 2743 2744 2745Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2742.276 kb on + strandat 2742.276 kb on + strandat 2742.277 kb on - strandat 2742.292 kb on + strandat 2742.292 kb on + strandat 2742.293 kb on - strandat 2742.293 kb on - strandat 2742.293 kb on - strandat 2742.293 kb on - strandat 2742.295 kb on - strandat 2742.361 kb on + strandat 2742.757 kb on + strand, within PS417_12595at 2742.940 kb on + strand, within PS417_12595at 2743.132 kb on + strandat 2743.228 kb on + strandat 2743.327 kb on + strand, within PS417_12600at 2744.931 kb on + strandat 2744.931 kb on + strand, within PS417_12605at 2745.104 kb on + strandat 2745.104 kb on + strandat 2745.105 kb on - strandat 2745.105 kb on - strandat 2745.247 kb on + strand, within PS417_12610at 2745.247 kb on + strand, within PS417_12610at 2745.397 kb on - strand, within PS417_12610

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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2,742,276 + +0.7
2,742,276 + +1.1
2,742,277 - +1.6
2,742,292 + -1.0
2,742,292 + -0.7
2,742,293 - +0.0
2,742,293 - -0.3
2,742,293 - +1.4
2,742,293 - -0.9
2,742,295 - +0.7
2,742,361 + -1.8
2,742,757 + PS417_12595 0.42 +1.9
2,742,940 + PS417_12595 0.68 +2.9
2,743,132 + +2.6
2,743,228 + -1.2
2,743,327 + PS417_12600 0.12 -0.9
2,744,931 + -0.9
2,744,931 + PS417_12605 0.69 -1.6
2,745,104 + -0.3
2,745,104 + +0.2
2,745,105 - -2.8
2,745,105 - -0.1
2,745,247 + PS417_12610 0.22 +2.4
2,745,247 + PS417_12610 0.22 -0.2
2,745,397 - PS417_12610 0.51 -0.3

Or see this region's nucleotide sequence