Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12590 and PS417_12595 are separated by 287 nucleotides PS417_12595 and PS417_12600 are separated by 3 nucleotides PS417_12600 and PS417_12605 are separated by 0 nucleotides PS417_12605 and PS417_12610 overlap by 4 nucleotides
PS417_12590: PS417_12590 - RND transporter, at 2,741,033 to 2,742,175
_12590
PS417_12595: PS417_12595 - transcriptional regulator, at 2,742,463 to 2,743,164
_12595
PS417_12600: PS417_12600 - histidine kinase, at 2,743,168 to 2,744,469
_12600
PS417_12605: PS417_12605 - RNA polymerase subunit sigma-70, at 2,744,470 to 2,745,138
_12605
PS417_12610: PS417_12610 - RNA polymerase sigma factor, at 2,745,135 to 2,745,650
_12610
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2742.276 kb on + strand at 2742.276 kb on + strand at 2742.277 kb on - strand at 2742.292 kb on + strand at 2742.292 kb on + strand at 2742.293 kb on - strand at 2742.293 kb on - strand at 2742.293 kb on - strand at 2742.293 kb on - strand at 2742.295 kb on - strand at 2742.361 kb on + strand at 2742.757 kb on + strand, within PS417_12595 at 2742.940 kb on + strand, within PS417_12595 at 2743.132 kb on + strand at 2743.228 kb on + strand at 2743.327 kb on + strand, within PS417_12600 at 2744.931 kb on + strand at 2744.931 kb on + strand, within PS417_12605 at 2745.104 kb on + strand at 2745.104 kb on + strand at 2745.105 kb on - strand at 2745.105 kb on - strand at 2745.247 kb on + strand, within PS417_12610 at 2745.247 kb on + strand, within PS417_12610 at 2745.397 kb on - strand, within PS417_12610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,742,276 + +0.7 2,742,276 + +1.1 2,742,277 - +1.6 2,742,292 + -1.0 2,742,292 + -0.7 2,742,293 - +0.0 2,742,293 - -0.3 2,742,293 - +1.4 2,742,293 - -0.9 2,742,295 - +0.7 2,742,361 + -1.8 2,742,757 + PS417_12595 0.42 +1.9 2,742,940 + PS417_12595 0.68 +2.9 2,743,132 + +2.6 2,743,228 + -1.2 2,743,327 + PS417_12600 0.12 -0.9 2,744,931 + -0.9 2,744,931 + PS417_12605 0.69 -1.6 2,745,104 + -0.3 2,745,104 + +0.2 2,745,105 - -2.8 2,745,105 - -0.1 2,745,247 + PS417_12610 0.22 +2.4 2,745,247 + PS417_12610 0.22 -0.2 2,745,397 - PS417_12610 0.51 -0.3
Or see this region's nucleotide sequence