Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12580 and PS417_12585 are separated by 151 nucleotides PS417_12585 and PS417_12590 are separated by 6 nucleotides
PS417_12580: PS417_12580 - membrane protein, at 2,737,141 to 2,737,782
_12580
PS417_12585: PS417_12585 - acriflavine resistance protein B, at 2,737,934 to 2,741,026
_12585
PS417_12590: PS417_12590 - RND transporter, at 2,741,033 to 2,742,175
_12590
Position (kb)
2737
2738
2739
2740
2741
2742 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2737.085 kb on + strand at 2737.085 kb on + strand at 2737.086 kb on - strand at 2737.086 kb on - strand at 2737.124 kb on - strand at 2737.165 kb on - strand at 2737.327 kb on - strand, within PS417_12580 at 2737.377 kb on - strand, within PS417_12580 at 2737.377 kb on - strand, within PS417_12580 at 2737.377 kb on - strand, within PS417_12580 at 2737.380 kb on - strand, within PS417_12580 at 2737.392 kb on - strand, within PS417_12580 at 2737.600 kb on + strand, within PS417_12580 at 2737.649 kb on + strand, within PS417_12580 at 2737.650 kb on - strand, within PS417_12580 at 2737.650 kb on - strand, within PS417_12580 at 2737.802 kb on + strand at 2737.803 kb on - strand at 2737.866 kb on - strand at 2738.036 kb on - strand at 2738.325 kb on - strand, within PS417_12585 at 2739.322 kb on - strand, within PS417_12585 at 2739.399 kb on + strand, within PS417_12585 at 2739.400 kb on - strand, within PS417_12585 at 2739.454 kb on - strand, within PS417_12585 at 2739.454 kb on - strand, within PS417_12585 at 2739.631 kb on - strand, within PS417_12585 at 2739.937 kb on + strand, within PS417_12585 at 2739.937 kb on + strand at 2739.937 kb on + strand, within PS417_12585 at 2740.004 kb on - strand, within PS417_12585 at 2740.005 kb on + strand, within PS417_12585 at 2740.005 kb on + strand, within PS417_12585 at 2740.005 kb on + strand, within PS417_12585 at 2740.005 kb on + strand, within PS417_12585 at 2740.006 kb on - strand, within PS417_12585 at 2740.006 kb on - strand, within PS417_12585 at 2740.006 kb on - strand, within PS417_12585 at 2740.063 kb on - strand, within PS417_12585 at 2740.158 kb on - strand, within PS417_12585 at 2740.206 kb on + strand, within PS417_12585 at 2740.497 kb on + strand, within PS417_12585 at 2740.798 kb on - strand at 2740.882 kb on - strand at 2740.884 kb on - strand at 2740.890 kb on + strand at 2741.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,737,085 + -2.5 2,737,085 + +0.2 2,737,086 - +1.3 2,737,086 - +1.3 2,737,124 - -1.5 2,737,165 - +0.5 2,737,327 - PS417_12580 0.29 -1.4 2,737,377 - PS417_12580 0.37 -1.2 2,737,377 - PS417_12580 0.37 +0.7 2,737,377 - PS417_12580 0.37 +0.5 2,737,380 - PS417_12580 0.37 -1.5 2,737,392 - PS417_12580 0.39 -0.0 2,737,600 + PS417_12580 0.71 -0.5 2,737,649 + PS417_12580 0.79 -0.4 2,737,650 - PS417_12580 0.79 -2.3 2,737,650 - PS417_12580 0.79 +0.4 2,737,802 + +2.4 2,737,803 - +0.1 2,737,866 - +1.2 2,738,036 - +1.4 2,738,325 - PS417_12585 0.13 -3.3 2,739,322 - PS417_12585 0.45 -2.9 2,739,399 + PS417_12585 0.47 -2.9 2,739,400 - PS417_12585 0.47 -2.1 2,739,454 - PS417_12585 0.49 -2.9 2,739,454 - PS417_12585 0.49 -3.9 2,739,631 - PS417_12585 0.55 -0.9 2,739,937 + PS417_12585 0.65 -2.5 2,739,937 + -1.2 2,739,937 + PS417_12585 0.65 -1.2 2,740,004 - PS417_12585 0.67 -0.8 2,740,005 + PS417_12585 0.67 -2.1 2,740,005 + PS417_12585 0.67 -3.0 2,740,005 + PS417_12585 0.67 -4.4 2,740,005 + PS417_12585 0.67 -2.9 2,740,006 - PS417_12585 0.67 -0.7 2,740,006 - PS417_12585 0.67 -2.0 2,740,006 - PS417_12585 0.67 -2.2 2,740,063 - PS417_12585 0.69 -0.9 2,740,158 - PS417_12585 0.72 -2.4 2,740,206 + PS417_12585 0.73 -0.2 2,740,497 + PS417_12585 0.83 -2.3 2,740,798 - -0.5 2,740,882 - -1.5 2,740,884 - -0.9 2,740,890 + -3.2 2,741,012 - -1.9
Or see this region's nucleotide sequence