Strain Fitness in Pseudomonas simiae WCS417 around PS417_01250

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01245 and PS417_01250 are separated by 284 nucleotidesPS417_01250 and PS417_01255 are separated by 22 nucleotidesPS417_01255 and PS417_01260 are separated by 25 nucleotides PS417_01245: PS417_01245 - hypothetical protein, at 270,041 to 271,216 _01245 PS417_01250: PS417_01250 - ATP-dependent DNA helicase RecQ, at 271,501 to 273,438 _01250 PS417_01255: PS417_01255 - thioesterase, at 273,461 to 273,892 _01255 PS417_01260: PS417_01260 - hypothetical protein, at 273,918 to 274,397 _01260 Position (kb) 271 272 273 274Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 270.536 kb on + strand, within PS417_01245at 270.548 kb on + strand, within PS417_01245at 270.548 kb on + strand, within PS417_01245at 270.549 kb on - strand, within PS417_01245at 270.549 kb on - strand, within PS417_01245at 270.549 kb on - strand, within PS417_01245at 270.549 kb on - strand, within PS417_01245at 270.549 kb on - strand, within PS417_01245at 270.555 kb on - strand, within PS417_01245at 270.555 kb on - strand, within PS417_01245at 270.633 kb on - strand, within PS417_01245at 270.633 kb on - strand, within PS417_01245at 270.633 kb on - strand, within PS417_01245at 270.633 kb on - strand, within PS417_01245at 270.655 kb on + strand, within PS417_01245at 270.659 kb on + strand, within PS417_01245at 270.659 kb on + strand, within PS417_01245at 270.662 kb on + strand, within PS417_01245at 270.662 kb on + strand, within PS417_01245at 270.662 kb on + strand, within PS417_01245at 270.843 kb on + strand, within PS417_01245at 270.844 kb on - strand, within PS417_01245at 270.986 kb on + strand, within PS417_01245at 271.085 kb on + strand, within PS417_01245at 271.085 kb on + strand, within PS417_01245at 271.085 kb on + strand, within PS417_01245at 271.088 kb on + strand, within PS417_01245at 271.088 kb on + strand, within PS417_01245at 271.089 kb on - strand, within PS417_01245at 271.106 kb on + strandat 271.107 kb on - strandat 271.225 kb on + strandat 271.225 kb on + strandat 271.225 kb on + strandat 271.226 kb on - strandat 271.283 kb on + strandat 271.283 kb on + strandat 271.284 kb on - strandat 271.284 kb on - strandat 271.284 kb on - strandat 271.284 kb on - strandat 271.284 kb on - strandat 271.347 kb on + strandat 271.348 kb on - strandat 271.348 kb on - strandat 271.348 kb on - strandat 271.348 kb on - strandat 271.402 kb on + strandat 271.402 kb on + strandat 271.402 kb on + strandat 271.402 kb on + strandat 271.402 kb on + strandat 271.403 kb on - strandat 271.403 kb on - strandat 271.403 kb on - strandat 271.403 kb on - strandat 271.460 kb on - strandat 271.474 kb on - strandat 271.633 kb on + strandat 271.731 kb on + strand, within PS417_01250at 271.731 kb on + strand, within PS417_01250at 271.747 kb on - strand, within PS417_01250at 271.915 kb on - strand, within PS417_01250at 271.967 kb on - strand, within PS417_01250at 272.089 kb on + strand, within PS417_01250at 272.428 kb on + strand, within PS417_01250at 272.428 kb on + strand, within PS417_01250at 272.428 kb on + strand, within PS417_01250at 272.428 kb on + strand, within PS417_01250at 272.717 kb on - strand, within PS417_01250at 272.737 kb on + strand, within PS417_01250at 272.738 kb on - strand, within PS417_01250at 272.909 kb on - strand, within PS417_01250at 272.963 kb on - strand, within PS417_01250at 272.997 kb on - strand, within PS417_01250at 273.011 kb on + strand, within PS417_01250at 273.013 kb on + strand, within PS417_01250at 273.014 kb on - strand, within PS417_01250at 273.014 kb on - strand, within PS417_01250at 273.014 kb on - strand, within PS417_01250at 273.116 kb on - strand, within PS417_01250at 273.219 kb on + strand, within PS417_01250at 273.250 kb on - strandat 273.391 kb on + strandat 273.391 kb on + strandat 273.496 kb on - strandat 273.539 kb on + strand, within PS417_01255at 273.539 kb on + strand, within PS417_01255at 273.539 kb on + strand, within PS417_01255at 273.540 kb on - strand, within PS417_01255at 273.540 kb on - strand, within PS417_01255at 273.563 kb on + strand, within PS417_01255at 273.564 kb on - strand, within PS417_01255at 273.564 kb on - strand, within PS417_01255at 273.564 kb on - strand, within PS417_01255at 273.566 kb on + strand, within PS417_01255at 273.567 kb on - strand, within PS417_01255at 273.593 kb on - strand, within PS417_01255at 273.593 kb on - strand, within PS417_01255at 273.593 kb on - strand, within PS417_01255at 273.593 kb on - strand, within PS417_01255at 273.593 kb on - strand, within PS417_01255at 273.596 kb on + strand, within PS417_01255at 273.597 kb on - strand, within PS417_01255at 273.597 kb on - strand, within PS417_01255at 273.671 kb on + strand, within PS417_01255at 273.672 kb on - strand, within PS417_01255at 273.737 kb on - strand, within PS417_01255at 273.737 kb on - strand, within PS417_01255at 273.737 kb on - strand, within PS417_01255at 273.737 kb on - strand, within PS417_01255at 273.737 kb on - strand, within PS417_01255at 273.750 kb on - strand, within PS417_01255at 273.905 kb on - strandat 273.905 kb on - strandat 273.958 kb on + strandat 273.995 kb on - strand, within PS417_01260at 274.054 kb on + strand, within PS417_01260at 274.054 kb on + strand, within PS417_01260at 274.054 kb on + strand, within PS417_01260at 274.054 kb on + strandat 274.055 kb on - strand, within PS417_01260at 274.055 kb on - strand, within PS417_01260at 274.055 kb on - strand, within PS417_01260at 274.055 kb on - strand, within PS417_01260at 274.055 kb on - strand, within PS417_01260at 274.059 kb on - strand, within PS417_01260at 274.135 kb on - strand, within PS417_01260at 274.290 kb on + strand, within PS417_01260at 274.291 kb on - strand, within PS417_01260at 274.291 kb on - strand, within PS417_01260at 274.399 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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270,536 + PS417_01245 0.42 -0.5
270,548 + PS417_01245 0.43 +0.1
270,548 + PS417_01245 0.43 -2.4
270,549 - PS417_01245 0.43 +0.5
270,549 - PS417_01245 0.43 +0.7
270,549 - PS417_01245 0.43 +0.1
270,549 - PS417_01245 0.43 -0.9
270,549 - PS417_01245 0.43 +1.8
270,555 - PS417_01245 0.44 +3.5
270,555 - PS417_01245 0.44 -0.6
270,633 - PS417_01245 0.50 +0.9
270,633 - PS417_01245 0.50 -0.3
270,633 - PS417_01245 0.50 -0.9
270,633 - PS417_01245 0.50 -0.1
270,655 + PS417_01245 0.52 -0.5
270,659 + PS417_01245 0.53 +0.8
270,659 + PS417_01245 0.53 -0.4
270,662 + PS417_01245 0.53 +1.6
270,662 + PS417_01245 0.53 +2.5
270,662 + PS417_01245 0.53 -0.4
270,843 + PS417_01245 0.68 -0.1
270,844 - PS417_01245 0.68 +0.6
270,986 + PS417_01245 0.80 +0.5
271,085 + PS417_01245 0.89 -0.7
271,085 + PS417_01245 0.89 -0.0
271,085 + PS417_01245 0.89 -2.4
271,088 + PS417_01245 0.89 -1.1
271,088 + PS417_01245 0.89 +0.5
271,089 - PS417_01245 0.89 -1.2
271,106 + -1.6
271,107 - +0.6
271,225 + +0.8
271,225 + +2.2
271,225 + -3.5
271,226 - +0.2
271,283 + -0.6
271,283 + -0.6
271,284 - +0.7
271,284 - +0.4
271,284 - -0.3
271,284 - -1.9
271,284 - +0.1
271,347 + +0.9
271,348 - -2.9
271,348 - +2.5
271,348 - -0.0
271,348 - -0.1
271,402 + -1.1
271,402 + -0.2
271,402 + +0.2
271,402 + -1.4
271,402 + +0.0
271,403 - -1.5
271,403 - -0.9
271,403 - +1.3
271,403 - +2.1
271,460 - +0.1
271,474 - -0.5
271,633 + -2.0
271,731 + PS417_01250 0.12 +1.0
271,731 + PS417_01250 0.12 +0.1
271,747 - PS417_01250 0.13 -2.1
271,915 - PS417_01250 0.21 +1.5
271,967 - PS417_01250 0.24 +0.2
272,089 + PS417_01250 0.30 +2.2
272,428 + PS417_01250 0.48 -0.1
272,428 + PS417_01250 0.48 -0.3
272,428 + PS417_01250 0.48 +0.7
272,428 + PS417_01250 0.48 +0.6
272,717 - PS417_01250 0.63 +0.3
272,737 + PS417_01250 0.64 +1.3
272,738 - PS417_01250 0.64 +0.1
272,909 - PS417_01250 0.73 +1.5
272,963 - PS417_01250 0.75 +1.1
272,997 - PS417_01250 0.77 -3.0
273,011 + PS417_01250 0.78 -0.6
273,013 + PS417_01250 0.78 +1.5
273,014 - PS417_01250 0.78 -0.8
273,014 - PS417_01250 0.78 +3.3
273,014 - PS417_01250 0.78 -0.5
273,116 - PS417_01250 0.83 +0.9
273,219 + PS417_01250 0.89 -0.1
273,250 - -0.2
273,391 + +0.4
273,391 + +1.2
273,496 - -1.5
273,539 + PS417_01255 0.18 -0.9
273,539 + PS417_01255 0.18 -1.8
273,539 + PS417_01255 0.18 +0.4
273,540 - PS417_01255 0.18 -1.2
273,540 - PS417_01255 0.18 -0.1
273,563 + PS417_01255 0.24 +0.0
273,564 - PS417_01255 0.24 +0.1
273,564 - PS417_01255 0.24 +1.3
273,564 - PS417_01255 0.24 -0.5
273,566 + PS417_01255 0.24 +0.4
273,567 - PS417_01255 0.25 +1.9
273,593 - PS417_01255 0.31 +1.2
273,593 - PS417_01255 0.31 +0.5
273,593 - PS417_01255 0.31 +0.2
273,593 - PS417_01255 0.31 -0.5
273,593 - PS417_01255 0.31 +1.3
273,596 + PS417_01255 0.31 +2.9
273,597 - PS417_01255 0.31 +0.8
273,597 - PS417_01255 0.31 +0.3
273,671 + PS417_01255 0.49 -0.2
273,672 - PS417_01255 0.49 -1.4
273,737 - PS417_01255 0.64 -2.3
273,737 - PS417_01255 0.64 +0.2
273,737 - PS417_01255 0.64 -0.4
273,737 - PS417_01255 0.64 -0.9
273,737 - PS417_01255 0.64 +0.1
273,750 - PS417_01255 0.67 -0.5
273,905 - +0.5
273,905 - -1.4
273,958 + -1.0
273,995 - PS417_01260 0.16 -2.5
274,054 + PS417_01260 0.28 +0.1
274,054 + PS417_01260 0.28 -0.3
274,054 + PS417_01260 0.28 -1.3
274,054 + -1.4
274,055 - PS417_01260 0.29 +0.1
274,055 - PS417_01260 0.29 -0.9
274,055 - PS417_01260 0.29 -0.6
274,055 - PS417_01260 0.29 -0.3
274,055 - PS417_01260 0.29 -0.3
274,059 - PS417_01260 0.29 -1.3
274,135 - PS417_01260 0.45 -2.1
274,290 + PS417_01260 0.78 -0.9
274,291 - PS417_01260 0.78 -0.2
274,291 - PS417_01260 0.78 -0.1
274,399 - +0.3

Or see this region's nucleotide sequence