Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11755 and PS417_11760 are separated by 49 nucleotides PS417_11760 and PS417_11765 are separated by 309 nucleotides
PS417_11755: PS417_11755 - diacetyl reductase, at 2,557,356 to 2,558,135
_11755
PS417_11760: PS417_11760 - Fis family transcriptional regulator, at 2,558,185 to 2,560,050
_11760
PS417_11765: PS417_11765 - ABC transporter substrate-binding protein, at 2,560,360 to 2,561,337
_11765
Position (kb)
2558
2559
2560
2561 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2557.206 kb on - strand at 2557.211 kb on + strand at 2557.211 kb on + strand at 2557.211 kb on + strand at 2557.211 kb on + strand at 2557.212 kb on - strand at 2557.212 kb on - strand at 2557.212 kb on - strand at 2557.321 kb on - strand at 2557.321 kb on - strand at 2557.696 kb on + strand, within PS417_11755 at 2557.916 kb on - strand, within PS417_11755 at 2557.929 kb on - strand, within PS417_11755 at 2557.929 kb on - strand at 2557.998 kb on + strand, within PS417_11755 at 2557.999 kb on - strand, within PS417_11755 at 2558.891 kb on + strand, within PS417_11760 at 2559.236 kb on - strand, within PS417_11760 at 2559.690 kb on - strand, within PS417_11760 at 2560.051 kb on - strand at 2560.079 kb on + strand at 2560.079 kb on + strand at 2560.092 kb on - strand at 2560.307 kb on + strand at 2560.308 kb on - strand at 2560.308 kb on - strand at 2560.399 kb on + strand at 2560.400 kb on - strand at 2560.621 kb on + strand, within PS417_11765 at 2560.622 kb on - strand, within PS417_11765 at 2560.641 kb on - strand, within PS417_11765 at 2560.892 kb on - strand, within PS417_11765
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,557,206 - +0.2 2,557,211 + +1.0 2,557,211 + -0.5 2,557,211 + +0.5 2,557,211 + -0.7 2,557,212 - +0.5 2,557,212 - -1.8 2,557,212 - +0.1 2,557,321 - +0.6 2,557,321 - +0.2 2,557,696 + PS417_11755 0.44 +2.4 2,557,916 - PS417_11755 0.72 -1.4 2,557,929 - PS417_11755 0.73 -1.6 2,557,929 - -0.9 2,557,998 + PS417_11755 0.82 -0.3 2,557,999 - PS417_11755 0.82 +0.5 2,558,891 + PS417_11760 0.38 -0.8 2,559,236 - PS417_11760 0.56 -0.7 2,559,690 - PS417_11760 0.81 -0.6 2,560,051 - -4.1 2,560,079 + +0.2 2,560,079 + +1.3 2,560,092 - -2.1 2,560,307 + -1.2 2,560,308 - -0.5 2,560,308 - -1.9 2,560,399 + +0.6 2,560,400 - -2.8 2,560,621 + PS417_11765 0.27 -0.1 2,560,622 - PS417_11765 0.27 +0.4 2,560,641 - PS417_11765 0.29 -2.1 2,560,892 - PS417_11765 0.54 +0.1
Or see this region's nucleotide sequence