Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11355 and PS417_11360 are separated by 3 nucleotides PS417_11360 and PS417_11365 are separated by 42 nucleotides PS417_11365 and PS417_11370 are separated by 18 nucleotides PS417_11370 and PS417_11375 are separated by 14 nucleotides
PS417_11355: PS417_11355 - biopolymer transporter ExbD, at 2,446,725 to 2,447,150
_11355
PS417_11360: PS417_11360 - energy transducer TonB, at 2,447,154 to 2,447,858
_11360
PS417_11365: PS417_11365 - membrane protein, at 2,447,901 to 2,449,601
_11365
PS417_11370: PS417_11370 - DNA repair protein, at 2,449,620 to 2,450,246
_11370
PS417_11375: PS417_11375 - hypothetical protein, at 2,450,261 to 2,450,719
_11375
Position (kb)
2447
2448
2449
2450 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2447.038 kb on + strand at 2447.039 kb on - strand, within PS417_11355 at 2447.596 kb on - strand, within PS417_11360 at 2447.598 kb on - strand, within PS417_11360 at 2447.619 kb on + strand, within PS417_11360 at 2447.896 kb on + strand at 2447.897 kb on - strand at 2447.897 kb on - strand at 2448.172 kb on - strand, within PS417_11365 at 2448.367 kb on - strand, within PS417_11365 at 2448.804 kb on + strand at 2448.805 kb on - strand, within PS417_11365 at 2448.828 kb on - strand, within PS417_11365 at 2448.933 kb on + strand, within PS417_11365 at 2448.937 kb on - strand, within PS417_11365 at 2449.390 kb on - strand, within PS417_11365 at 2449.395 kb on + strand, within PS417_11365 at 2449.396 kb on - strand, within PS417_11365 at 2449.396 kb on - strand, within PS417_11365 at 2449.396 kb on - strand, within PS417_11365 at 2449.396 kb on - strand, within PS417_11365 at 2449.470 kb on + strand at 2449.546 kb on - strand at 2449.546 kb on - strand at 2450.130 kb on - strand, within PS417_11370 at 2450.248 kb on - strand at 2450.438 kb on + strand, within PS417_11375 at 2450.438 kb on + strand, within PS417_11375 at 2450.439 kb on - strand, within PS417_11375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,447,038 + +3.4 2,447,039 - PS417_11355 0.74 -0.8 2,447,596 - PS417_11360 0.63 -0.3 2,447,598 - PS417_11360 0.63 -0.4 2,447,619 + PS417_11360 0.66 -0.7 2,447,896 + +0.4 2,447,897 - +1.0 2,447,897 - +0.3 2,448,172 - PS417_11365 0.16 -1.1 2,448,367 - PS417_11365 0.27 -0.6 2,448,804 + +2.0 2,448,805 - PS417_11365 0.53 -0.2 2,448,828 - PS417_11365 0.54 +0.2 2,448,933 + PS417_11365 0.61 +0.6 2,448,937 - PS417_11365 0.61 -0.6 2,449,390 - PS417_11365 0.88 -2.5 2,449,395 + PS417_11365 0.88 -0.8 2,449,396 - PS417_11365 0.88 -1.0 2,449,396 - PS417_11365 0.88 -2.4 2,449,396 - PS417_11365 0.88 -1.1 2,449,396 - PS417_11365 0.88 +0.2 2,449,470 + -1.3 2,449,546 - -1.3 2,449,546 - -0.8 2,450,130 - PS417_11370 0.81 -1.0 2,450,248 - -0.4 2,450,438 + PS417_11375 0.39 +0.8 2,450,438 + PS417_11375 0.39 +0.3 2,450,439 - PS417_11375 0.39 -0.5
Or see this region's nucleotide sequence